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P84086

- CPLX2_MOUSE

UniProt

P84086 - CPLX2_MOUSE

Protein

Complexin-2

Gene

Cplx2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in synaptic vesicle exocytosis. Also involved in mast cell exocytosis. Although not essential for development, seems critical for the acquisition of higher cognitive functions in the adult brain.3 Publications

    GO - Molecular functioni

    1. syntaxin-1 binding Source: MGI

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. mast cell degranulation Source: UniProtKB-KW
    3. nervous system development Source: UniProtKB-KW
    4. positive regulation of synaptic plasticity Source: UniProtKB
    5. synaptic vesicle exocytosis Source: Ensembl

    Keywords - Biological processi

    Differentiation, Exocytosis, Mast cell degranulation, Neurogenesis, Neurotransmitter transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Complexin-2
    Alternative name(s):
    921-L
    Complexin II
    Short name:
    CPX II
    Synaphin-1
    Gene namesi
    Name:Cplx2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:104726. Cplx2.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication
    Note: Enriched at synaptic-releasing sites in mature neurons.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. dendrite Source: MGI
    3. mast cell granule Source: GOC
    4. neuronal cell body Source: MGI
    5. synapse Source: MGI
    6. synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Mice show no obvious phenotypic changes and no significant motor deficiencies. However, they show abnormalities in a number of complex behaviors including exploration, socialization, motor coordination, learning and reversal learning.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 134134Complexin-2PRO_0000144876Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei93 – 931PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP84086.
    PaxDbiP84086.
    PRIDEiP84086.

    PTM databases

    PhosphoSiteiP84086.

    Expressioni

    Tissue specificityi

    Nervous system. Expressed predominantly in brain, where it is present in many regions, including hippocampus and cerebellum. In the retina, present at conventional amacrine cell synapses (at protein level).2 Publications

    Developmental stagei

    In the brain, expression starts at P6 and increases to reach a plateau at P20.1 Publication

    Gene expression databases

    BgeeiP84086.
    CleanExiMM_CPLX2.
    GenevestigatoriP84086.

    Interactioni

    Subunit structurei

    Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.By similarity

    Protein-protein interaction databases

    IntActiP84086. 1 interaction.
    MINTiMINT-4153241.

    Structurei

    3D structure databases

    ProteinModelPortaliP84086.
    SMRiP84086. Positions 32-72.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 9757Interaction with the SNARE complexBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili28 – 8457Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complexin/synaphin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG85303.
    GeneTreeiENSGT00490000043416.
    HOGENOMiHOG000017716.
    HOVERGENiHBG062792.
    InParanoidiP84086.
    KOiK15294.
    OMAiARMENTT.
    OrthoDBiEOG71G9XG.
    PhylomeDBiP84086.
    TreeFamiTF315172.

    Family and domain databases

    InterProiIPR008849. Synaphin.
    [Graphical view]
    PANTHERiPTHR16705. PTHR16705. 1 hit.
    PfamiPF05835. Synaphin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P84086-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFVMKQALG GATKDMGKML GGEEEKDPDA QKKEEERQEA LRQQEEERKA    50
    KHARMEAERE KVRQQIRDKY GLKKKEEKEA EEKAALEQPC EGSLTRPKKA 100
    IPAGCGDEEE EEEESILDTV LKYLPGPLQD MFKK 134
    Length:134
    Mass (Da):15,394
    Last modified:August 16, 2004 - v1
    Checksum:i7791812FD4194AC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35101 Genomic DNA. Translation: AAC52272.1.
    D38613 mRNA. Translation: BAA07604.1.
    BN000501 mRNA. Translation: CAG26661.1.
    BN000502 mRNA. Translation: CAG26662.1.
    CCDSiCCDS36667.1.
    PIRiS66293. D57233.
    RefSeqiNP_034076.1. NM_009946.3.
    UniGeneiMm.268902.

    Genome annotation databases

    EnsembliENSMUST00000026985; ENSMUSP00000026985; ENSMUSG00000025867.
    GeneIDi12890.
    KEGGimmu:12890.
    UCSCiuc029sbi.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35101 Genomic DNA. Translation: AAC52272.1 .
    D38613 mRNA. Translation: BAA07604.1 .
    BN000501 mRNA. Translation: CAG26661.1 .
    BN000502 mRNA. Translation: CAG26662.1 .
    CCDSi CCDS36667.1.
    PIRi S66293. D57233.
    RefSeqi NP_034076.1. NM_009946.3.
    UniGenei Mm.268902.

    3D structure databases

    ProteinModelPortali P84086.
    SMRi P84086. Positions 32-72.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P84086. 1 interaction.
    MINTi MINT-4153241.

    PTM databases

    PhosphoSitei P84086.

    Proteomic databases

    MaxQBi P84086.
    PaxDbi P84086.
    PRIDEi P84086.

    Protocols and materials databases

    DNASUi 12890.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026985 ; ENSMUSP00000026985 ; ENSMUSG00000025867 .
    GeneIDi 12890.
    KEGGi mmu:12890.
    UCSCi uc029sbi.1. mouse.

    Organism-specific databases

    CTDi 10814.
    MGIi MGI:104726. Cplx2.

    Phylogenomic databases

    eggNOGi NOG85303.
    GeneTreei ENSGT00490000043416.
    HOGENOMi HOG000017716.
    HOVERGENi HBG062792.
    InParanoidi P84086.
    KOi K15294.
    OMAi ARMENTT.
    OrthoDBi EOG71G9XG.
    PhylomeDBi P84086.
    TreeFami TF315172.

    Miscellaneous databases

    ChiTaRSi CPLX2. mouse.
    NextBioi 282490.
    PROi P84086.
    SOURCEi Search...

    Gene expression databases

    Bgeei P84086.
    CleanExi MM_CPLX2.
    Genevestigatori P84086.

    Family and domain databases

    InterProi IPR008849. Synaphin.
    [Graphical view ]
    PANTHERi PTHR16705. PTHR16705. 1 hit.
    Pfami PF05835. Synaphin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complexins: cytosolic proteins that regulate SNAP receptor function."
      McMahon H.T., Missler M., Li C., Suedhof T.C.
      Cell 83:111-119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129/Sv.
      Tissue: Brain.
    2. "Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses."
      Takahashi S., Yamamoto H., Matsuda Z., Ogawa M., Yagyu K., Taniguchi T., Miyata T., Kaba H., Higuchi T., Okutani F., Fujimoto S.
      FEBS Lett. 368:455-460(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: BALB/c.
      Tissue: Brain.
    3. "Structural organization of the human complexin 2 gene (CPLX2) and aspects of its functional activity."
      Raevskaya N.M., Dergunova L.V., Vladychenskaya I.P., Stavchansky V.V., Oborina M.V., Poltaraus A.B., Limborska S.A.
      Gene 359:127-137(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
      Tissue: Brain.
    4. "Complexins regulate a late step in Ca2+-dependent neurotransmitter release."
      Reim K., Mansour M., Varoqueaux F., McMahon H.T., Suedhof T.C., Brose N., Rosenmund C.
      Cell 104:71-81(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Complexin II is essential for normal neurological function in mice."
      Glynn D., Bortnick R.A., Morton A.J.
      Hum. Mol. Genet. 12:2431-2448(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Structurally and functionally unique complexins at retinal ribbon synapses."
      Reim K., Wegmeyer H., Brandstaetter J.H., Xue M., Rosenmund C., Dresbach T., Hofmann K., Brose N.
      J. Cell Biol. 169:669-680(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    7. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    8. "Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
      Ravanpay A.C., Hansen S.J., Olson J.M.
      Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCPLX2_MOUSE
    AccessioniPrimary (citable) accession number: P84086
    Secondary accession number(s): O09056
    , Q13329, Q28184, Q32KK4, Q64386
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3