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P84086 (CPLX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Complexin-2
Alternative name(s):
921-L
Complexin II
Short name=CPX II
Synaphin-1
Gene names
Name:Cplx2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length134 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in synaptic vesicle exocytosis. Also involved in mast cell exocytosis. Although not essential for development, seems critical for the acquisition of higher cognitive functions in the adult brain. Ref.4 Ref.5 Ref.8

Subunit structure

Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A By similarity.

Subcellular location

Cytoplasmcytosol. Note: Enriched at synaptic-releasing sites in mature neurons. Ref.2

Tissue specificity

Nervous system. Expressed predominantly in brain, where it is present in many regions, including hippocampus and cerebellum. In the retina, present at conventional amacrine cell synapses (at protein level). Ref.2 Ref.6

Developmental stage

In the brain, expression starts at P6 and increases to reach a plateau at P20. Ref.6

Disruption phenotype

Mice show no obvious phenotypic changes and no significant motor deficiencies. However, they show abnormalities in a number of complex behaviors including exploration, socialization, motor coordination, learning and reversal learning. Ref.5

Sequence similarities

Belongs to the complexin/synaphin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 134134Complexin-2
PRO_0000144876

Regions

Region41 – 9757Interaction with the SNARE complex By similarity
Coiled coil28 – 8457 Potential

Amino acid modifications

Modified residue931Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P84086 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 7791812FD4194AC5

FASTA13415,394
        10         20         30         40         50         60 
MDFVMKQALG GATKDMGKML GGEEEKDPDA QKKEEERQEA LRQQEEERKA KHARMEAERE 

        70         80         90        100        110        120 
KVRQQIRDKY GLKKKEEKEA EEKAALEQPC EGSLTRPKKA IPAGCGDEEE EEEESILDTV 

       130 
LKYLPGPLQD MFKK 

« Hide

References

« Hide 'large scale' references
[1]"Complexins: cytosolic proteins that regulate SNAP receptor function."
McMahon H.T., Missler M., Li C., Suedhof T.C.
Cell 83:111-119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
Tissue: Brain.
[2]"Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses."
Takahashi S., Yamamoto H., Matsuda Z., Ogawa M., Yagyu K., Taniguchi T., Miyata T., Kaba H., Higuchi T., Okutani F., Fujimoto S.
FEBS Lett. 368:455-460(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: BALB/c.
Tissue: Brain.
[3]"Structural organization of the human complexin 2 gene (CPLX2) and aspects of its functional activity."
Raevskaya N.M., Dergunova L.V., Vladychenskaya I.P., Stavchansky V.V., Oborina M.V., Poltaraus A.B., Limborska S.A.
Gene 359:127-137(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
Tissue: Brain.
[4]"Complexins regulate a late step in Ca2+-dependent neurotransmitter release."
Reim K., Mansour M., Varoqueaux F., McMahon H.T., Suedhof T.C., Brose N., Rosenmund C.
Cell 104:71-81(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Complexin II is essential for normal neurological function in mice."
Glynn D., Bortnick R.A., Morton A.J.
Hum. Mol. Genet. 12:2431-2448(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"Structurally and functionally unique complexins at retinal ribbon synapses."
Reim K., Wegmeyer H., Brandstaetter J.H., Xue M., Rosenmund C., Dresbach T., Hofmann K., Brose N.
J. Cell Biol. 169:669-680(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[7]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[8]"Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
Ravanpay A.C., Hansen S.J., Olson J.M.
Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35101 Genomic DNA. Translation: AAC52272.1.
D38613 mRNA. Translation: BAA07604.1.
BN000501 mRNA. Translation: CAG26661.1.
BN000502 mRNA. Translation: CAG26662.1.
PIRD57233. S66293.
RefSeqNP_034076.1. NM_009946.2.
UniGeneMm.268902.

3D structure databases

ProteinModelPortalP84086.
SMRP84086. Positions 32-72.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP84086. 1 interaction.
MINTMINT-4153241.

PTM databases

PhosphoSiteP84086.

Proteomic databases

PaxDbP84086.
PRIDEP84086.

Protocols and materials databases

DNASU12890.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026985; ENSMUSP00000026985; ENSMUSG00000025867.
GeneID12890.
KEGGmmu:12890.
UCSCuc029sbi.1. mouse.

Organism-specific databases

CTD10814.
MGIMGI:104726. Cplx2.

Phylogenomic databases

eggNOGNOG85303.
GeneTreeENSGT00490000043416.
HOGENOMHOG000017716.
HOVERGENHBG062792.
InParanoidP84086.
KOK15294.
OMARATKDMG.
OrthoDBEOG71G9XG.
PhylomeDBP84086.
TreeFamTF315172.

Gene expression databases

BgeeP84086.
CleanExMM_CPLX2.
GenevestigatorP84086.

Family and domain databases

InterProIPR008849. Synaphin.
[Graphical view]
PANTHERPTHR16705. PTHR16705. 1 hit.
PfamPF05835. Synaphin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCPLX2. mouse.
NextBio282490.
PROP84086.
SOURCESearch...

Entry information

Entry nameCPLX2_MOUSE
AccessionPrimary (citable) accession number: P84086
Secondary accession number(s): O09056 expand/collapse secondary AC list , Q13329, Q28184, Q32KK4, Q64386
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot