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Protein

ADP-ribosylation factor 5

Gene

ARF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTP
Nucleotide bindingi67 – 715GTP
Nucleotide bindingi126 – 1294GTP

GO - Molecular functioni

  • GTPase activity Source: ProtInc
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: WormBase
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 5
Gene namesi
Name:ARF5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:658. ARF5.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24941.

Chemistry

ChEMBLiCHEMBL5986.

Polymorphism and mutation databases

BioMutaiARF5.
DMDMi51316990.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 180179ADP-ribosylation factor 5PRO_0000207396Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiP84085.
MaxQBiP84085.
PaxDbiP84085.
PeptideAtlasiP84085.
PRIDEiP84085.
TopDownProteomicsiP84085.

PTM databases

iPTMnetiP84085.
PhosphoSiteiP84085.
SwissPalmiP84085.

Expressioni

Gene expression databases

BgeeiP84085.
CleanExiHS_ARF5.
ExpressionAtlasiP84085. baseline and differential.
GenevisibleiP84085. HS.

Interactioni

Subunit structurei

Binds ASAP2. Interacts with NCS1/FREQ at the Golgi complex. Interacts with RAB11FIP3 and RAB11FIP4.4 Publications

Protein-protein interaction databases

BioGridi106876. 51 interactions.
DIPiDIP-61289N.
IntActiP84085. 4 interactions.
MINTiMINT-4825056.
STRINGi9606.ENSP00000000233.

Structurei

Secondary structure

1
180
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 114Combined sources
Turni12 – 154Combined sources
Beta strandi18 – 258Combined sources
Helixi30 – 378Combined sources
Beta strandi43 – 486Combined sources
Beta strandi51 – 588Combined sources
Beta strandi61 – 677Combined sources
Helixi78 – 836Combined sources
Beta strandi87 – 937Combined sources
Helixi97 – 993Combined sources
Helixi100 – 11112Combined sources
Helixi114 – 1163Combined sources
Beta strandi120 – 1267Combined sources
Helixi136 – 1427Combined sources
Helixi145 – 1473Combined sources
Beta strandi153 – 1575Combined sources
Turni160 – 1634Combined sources
Helixi166 – 17611Combined sources
Turni177 – 1793Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B6HX-ray1.76A8-180[»]
ProteinModelPortaliP84085.
SMRiP84085. Positions 7-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84085.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84085.
KOiK07940.
OMAiMVQEDEL.
OrthoDBiEOG77WWDV.
PhylomeDBiP84085.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS
160 170 180
RTWYVQATCA TQGTGLYDGL DWLSHELSKR
Length:180
Mass (Da):20,530
Last modified:January 23, 2007 - v2
Checksum:i25EF3D4895408147
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57567 mRNA. Translation: AAA90927.1.
U73002 Genomic DNA. Translation: AAC51299.1.
AF493884 mRNA. Translation: AAM12598.1.
BT007087 mRNA. Translation: AAP35750.1.
BC003043 mRNA. Translation: AAH03043.1.
BC033104 mRNA. Translation: AAH33104.1.
CCDSiCCDS34745.1.
PIRiA23741.
RefSeqiNP_001653.1. NM_001662.3.
UniGeneiHs.653202.

Genome annotation databases

EnsembliENST00000000233; ENSP00000000233; ENSG00000004059.
GeneIDi381.
KEGGihsa:381.
UCSCiuc003vmb.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57567 mRNA. Translation: AAA90927.1.
U73002 Genomic DNA. Translation: AAC51299.1.
AF493884 mRNA. Translation: AAM12598.1.
BT007087 mRNA. Translation: AAP35750.1.
BC003043 mRNA. Translation: AAH03043.1.
BC033104 mRNA. Translation: AAH33104.1.
CCDSiCCDS34745.1.
PIRiA23741.
RefSeqiNP_001653.1. NM_001662.3.
UniGeneiHs.653202.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B6HX-ray1.76A8-180[»]
ProteinModelPortaliP84085.
SMRiP84085. Positions 7-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106876. 51 interactions.
DIPiDIP-61289N.
IntActiP84085. 4 interactions.
MINTiMINT-4825056.
STRINGi9606.ENSP00000000233.

Chemistry

ChEMBLiCHEMBL5986.

PTM databases

iPTMnetiP84085.
PhosphoSiteiP84085.
SwissPalmiP84085.

Polymorphism and mutation databases

BioMutaiARF5.
DMDMi51316990.

Proteomic databases

EPDiP84085.
MaxQBiP84085.
PaxDbiP84085.
PeptideAtlasiP84085.
PRIDEiP84085.
TopDownProteomicsiP84085.

Protocols and materials databases

DNASUi381.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000000233; ENSP00000000233; ENSG00000004059.
GeneIDi381.
KEGGihsa:381.
UCSCiuc003vmb.3. human.

Organism-specific databases

CTDi381.
GeneCardsiARF5.
HGNCiHGNC:658. ARF5.
MIMi103188. gene.
neXtProtiNX_P84085.
PharmGKBiPA24941.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84085.
KOiK07940.
OMAiMVQEDEL.
OrthoDBiEOG77WWDV.
PhylomeDBiP84085.
TreeFamiTF300808.

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSiARF5. human.
EvolutionaryTraceiP84085.
GeneWikiiARF5.
GenomeRNAii381.
PROiP84085.
SOURCEiSearch...

Gene expression databases

BgeeiP84085.
CleanExiHS_ARF5.
ExpressionAtlasiP84085. baseline and differential.
GenevisibleiP84085. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells."
    Tsuchiya M., Price S.R., Tsai S.-C., Moss J., Vaughan M.
    J. Biol. Chem. 266:2772-2777(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Localization and characterization of the human ADP-ribosylation factor 5 (ARF5) gene."
    McGuire R.E., Daiger S.P., Green E.D.
    Genomics 41:481-484(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and Skin.
  6. "Identification of a new Pyk2 target protein with Arf-GAP activity."
    Andreev J., Simon J.-P., Sabatini D.D., Kam J., Plowman G., Randazzo P.A., Schlessinger J.
    Mol. Cell. Biol. 19:2338-2350(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ASAP2.
  7. "Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1."
    Shiba T., Koga H., Shin H.-W., Kawasaki M., Kato R., Nakayama K., Wakatsuki S.
    Proc. Natl. Acad. Sci. U.S.A. 103:15416-15421(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAB11FIP3 AND RAB11FIP4.
  8. "Specificity, promiscuity and localization of ARF protein interactions with NCS-1 and phosphatidylinositol-4 kinase-III beta."
    Haynes L.P., Sherwood M.W., Dolman N.J., Burgoyne R.D.
    Traffic 8:1080-1092(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NCS1, SUBCELLULAR LOCATION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global profiling of co- and post-translationally N-myristoylated proteomes in human cells."
    Thinon E., Serwa R.A., Broncel M., Brannigan J.A., Brassat U., Wright M.H., Heal W.P., Wilkinson A.J., Mann D.J., Tate E.W.
    Nat. Commun. 5:4919-4919(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT GLY-2, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
  11. "Structure of human ADP-ribosylation factor 5."
    Structural genomics consortium (SGC)
    Submitted (AUG-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 8-180 IN COMPLEX WITH GDP.

Entry informationi

Entry nameiARF5_HUMAN
AccessioniPrimary (citable) accession number: P84085
Secondary accession number(s): P26437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.