ADP-ribosylation factor 5 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P84085 (ARF5_HUMAN)

Last modified November 24, 2009. Version 63. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
ADP-ribosylation factor 5

Gene names

Name:

ARF5

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Subunit structure	Binds ASAP2. Interacts with FREQ/NCS1 at the Golgi complex. Ref.6 Ref.7
Subcellular location	Golgi apparatus. Cytoplasm › perinuclear region.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

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Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Potential

Chain

2 – 180

179

ADP-ribosylation factor 5

PRO_0000207396

Regions

Nucleotide binding

24 – 31

GTP

Nucleotide binding

67 – 71

GTP

Nucleotide binding

126 – 129

GTP

Amino acid modifications

Lipidation

N-myristoyl glycine Potential

Secondary structure

180

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P84085-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 25EF3D4895408147
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FASTA

180

20,530

        10         20         30         40         50         60 
MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN 

        70         80         90        100        110        120 
ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV 

       130        140        150        160        170        180 
LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells." Tsuchiya M., Price S.R., Tsai S.-C., Moss J., Vaughan M. J. Biol. Chem. 266:2772-2777(1991) [PubMed: 1993656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Localization and characterization of the human ADP-ribosylation factor 5 (ARF5) gene." McGuire R.E., Daiger S.P., Green E.D. Genomics 41:481-484(1997) [PubMed: 9169151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Skin.
[6]	"Identification of a new Pyk2 target protein with Arf-GAP activity." Andreev J., Simon J.-P., Sabatini D.D., Kam J., Plowman G., Randazzo P.A., Schlessinger J. Mol. Cell. Biol. 19:2338-2350(1999) [PubMed: 10022920] [Abstract] Cited for: INTERACTION WITH ASAP2.
[7]	"Specificity, promiscuity and localization of ARF protein interactions with NCS-1 and phosphatidylinositol-4 kinase-III beta." Haynes L.P., Sherwood M.W., Dolman N.J., Burgoyne R.D. Traffic 8:1080-1092(2007) [PubMed: 17555535] [Abstract] Cited for: INTERACTION WITH FREQ, SUBCELLULAR LOCATION.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Structure of human ADP-ribosylation factor 5." Structural genomics consortium (SGC) Submitted (AUG-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 8-180 IN COMPLEX WITH GDP.

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Cross-references

Sequence databases

M57567 mRNA. Translation: AAA90927.1.
U73002 Genomic DNA. Translation: AAC51299.1.
AF493884 mRNA. Translation: AAM12598.1.
BT007087 mRNA. Translation: AAP35750.1.
BC003043 mRNA. Translation: AAH03043.1.
BC033104 mRNA. Translation: AAH33104.1.

IPI

IPI00215919.

PIR

A23741.

RefSeq

NP_001653.1.

UniGene

Hs.653202

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B6H	X-ray	1.76	A	8-179	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P84085.

Proteomic databases

PeptideAtlas

P84085.

PRIDE

P84085.

Genome annotation databases

Ensembl

ENST00000000233; ENSP00000000233; ENSG00000004059; Homo sapiens. [Genome view]

GeneID

381.

KEGG

hsa:381.

UCSC

uc003vmb.1. human.

Organism-specific databases

CTD

381.

GeneCards

GC07P127015.

H-InvDB

HIX0007046.

HGNC

HGNC:658. ARF5.

MIM

103188. gene.

PharmGKB

PA24941.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P84085.

HOVERGEN

P84085.

OMA

GXLQEDE

Enzyme and pathway databases

Pathway_Interaction_DB

pi3kcipathway. Class I PI3K signaling events.

Gene expression databases

ArrayExpress

P84085.

Bgee

P84085.

CleanEx

HS_ARF5.

Genevestigator

P84085.

GermOnline

ENSG00000004059. Homo sapiens.

Family and domain databases

InterPro

IPR006688. ARF.
IPR006689. ARF/SAR.
IPR005225. Small_GTP_bd.
[Graphical view]

PANTHER

PTHR11711. ARF/SAR. 1 hit.

Pfam

PF00025. Arf. 1 hit.
[Graphical view]

PRINTS

PR00328. SAR1GTPBP.

SMART

SM00177. ARF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51417. ARF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

1595.

SOURCE

Search...

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Entry information

Entry name

ARF5_HUMAN

Accession

Primary (citable) accession number: P84085
Secondary accession number(s): P26437

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families