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Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160GTP; via amide nitrogenCombined sourcesCurated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 32GTPCombined sourcesCurated9
Nucleotide bindingi126 – 129GTPCombined sourcesCurated4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-1660499 Synthesis of PIPs at the plasma membrane
R-BTA-1660514 Synthesis of PIPs at the Golgi membrane
R-BTA-2132295 MHC class II antigen presentation
R-BTA-421837 Clathrin derived vesicle budding
R-BTA-432720 Lysosome Vesicle Biogenesis
R-BTA-432722 Golgi Associated Vesicle Biogenesis
R-BTA-6807878 COPI-mediated anterograde transport
R-BTA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-BTA-6811438 Intra-Golgi traffic

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:ARF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Organism-specific databases

VGNCiVGNC:50209 ARF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71Q → L: Mainly GTP-bound form; abolishes interaction with TMED10. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002073772 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine; alternateBy similarity1
Lipidationi2N-myristoyl glycine; alternateBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Myristate

Proteomic databases

PaxDbiP84080
PeptideAtlasiP84080
PRIDEiP84080

Expressioni

Gene expression databases

BgeeiENSBTAG00000007725

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition (By similarity). Interacts with IQSEC1 (PubMed:24058294). Interacts with C9orf72 (By similarity).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi160195, 1 interactor
IntActiP84080, 9 interactors
MINTiP84080
STRINGi9913.ENSBTAP00000010159

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 7Combined sources4
Beta strandi19 – 23Combined sources5
Helixi30 – 40Combined sources11
Beta strandi49 – 51Combined sources3
Beta strandi53 – 57Combined sources5
Beta strandi62 – 67Combined sources6
Helixi72 – 74Combined sources3
Helixi75 – 81Combined sources7
Turni82 – 84Combined sources3
Beta strandi86 – 93Combined sources8
Helixi97 – 99Combined sources3
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Helixi136 – 142Combined sources7
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 163Combined sources4
Helixi166 – 176Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R8QX-ray1.86A/B1-181[»]
1R8SX-ray1.46A18-181[»]
1S9DX-ray1.80A18-181[»]
4C0AX-ray3.30C/D/G/H18-181[»]
ProteinModelPortaliP84080
SMRiP84080
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84080

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP84080
KOiK07937
OMAiHYYSNTD
OrthoDBiEOG091G0S7S
TreeFamiTF300808

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Length:181
Mass (Da):20,697
Last modified:January 23, 2007 - v2
Checksum:iAAC773D4A60186B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03275 mRNA Translation: AAA30361.1
BC102874 mRNA Translation: AAI02875.1
BC140532 mRNA Translation: AAI40533.1
PIRiA36167
RefSeqiNP_788826.1, NM_176653.4
XP_005208456.1, XM_005208399.3
XP_005208457.1, XM_005208400.3
UniGeneiBt.91076
Bt.927

Genome annotation databases

EnsembliENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725
GeneIDi338058
KEGGibta:338058

Similar proteinsi

Entry informationi

Entry nameiARF1_BOVIN
AccessioniPrimary (citable) accession number: P84080
Secondary accession number(s): A5D7F1
, P10947, P32889, Q3SZG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health