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Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160GTP; via amide nitrogenCombined sourcesCurated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 32GTPCombined sourcesCurated9
Nucleotide bindingi126 – 129GTPCombined sourcesCurated4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-1660499. Synthesis of PIPs at the plasma membrane.
R-BTA-1660514. Synthesis of PIPs at the Golgi membrane.
R-BTA-2132295. MHC class II antigen presentation.
R-BTA-421837. Clathrin derived vesicle budding.
R-BTA-432720. Lysosome Vesicle Biogenesis.
R-BTA-432722. Golgi Associated Vesicle Biogenesis.
R-BTA-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:ARF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: Ensembl
  • cytosol Source: AgBase
  • extracellular exosome Source: Ensembl
  • focal adhesion Source: Ensembl
  • Golgi apparatus Source: AgBase
  • neuron projection Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: AgBase
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
  • sarcomere Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71Q → L: Mainly GTP-bound form; abolishes interaction with TMED10. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002073772 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine; alternateBy similarity1
Lipidationi2N-myristoyl glycine; alternateBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Myristate

Proteomic databases

PaxDbiP84080.
PeptideAtlasiP84080.
PRIDEiP84080.

Expressioni

Gene expression databases

BgeeiENSBTAG00000007725.

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition (By similarity). Interacts with IQSEC1 (PubMed:24058294).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ncs1P621682EBI-449051,EBI-907774From a different organism.

Protein-protein interaction databases

IntActiP84080. 8 interactors.
MINTiMINT-126253.
STRINGi9913.ENSBTAP00000010159.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 7Combined sources4
Beta strandi19 – 23Combined sources5
Helixi30 – 40Combined sources11
Beta strandi49 – 51Combined sources3
Beta strandi53 – 57Combined sources5
Beta strandi62 – 67Combined sources6
Helixi72 – 74Combined sources3
Helixi75 – 81Combined sources7
Turni82 – 84Combined sources3
Beta strandi86 – 93Combined sources8
Helixi97 – 99Combined sources3
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Helixi136 – 142Combined sources7
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 163Combined sources4
Helixi166 – 176Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R8QX-ray1.86A/B1-181[»]
1R8SX-ray1.46A18-181[»]
1S9DX-ray1.80A18-181[»]
4C0AX-ray3.30C/D/G/H18-181[»]
ProteinModelPortaliP84080.
SMRiP84080.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84080.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84080.
KOiK07937.
OMAiHAMSAPE.
OrthoDBiEOG091G0S7S.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Length:181
Mass (Da):20,697
Last modified:January 23, 2007 - v2
Checksum:iAAC773D4A60186B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03275 mRNA. Translation: AAA30361.1.
BC102874 mRNA. Translation: AAI02875.1.
BC140532 mRNA. Translation: AAI40533.1.
PIRiA36167.
RefSeqiNP_788826.1. NM_176653.4.
XP_005208456.1. XM_005208399.3.
XP_005208457.1. XM_005208400.3.
UniGeneiBt.91076.
Bt.927.

Genome annotation databases

EnsembliENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725.
GeneIDi338058.
KEGGibta:338058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03275 mRNA. Translation: AAA30361.1.
BC102874 mRNA. Translation: AAI02875.1.
BC140532 mRNA. Translation: AAI40533.1.
PIRiA36167.
RefSeqiNP_788826.1. NM_176653.4.
XP_005208456.1. XM_005208399.3.
XP_005208457.1. XM_005208400.3.
UniGeneiBt.91076.
Bt.927.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R8QX-ray1.86A/B1-181[»]
1R8SX-ray1.46A18-181[»]
1S9DX-ray1.80A18-181[»]
4C0AX-ray3.30C/D/G/H18-181[»]
ProteinModelPortaliP84080.
SMRiP84080.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP84080. 8 interactors.
MINTiMINT-126253.
STRINGi9913.ENSBTAP00000010159.

Proteomic databases

PaxDbiP84080.
PeptideAtlasiP84080.
PRIDEiP84080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725.
GeneIDi338058.
KEGGibta:338058.

Organism-specific databases

CTDi375.

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84080.
KOiK07937.
OMAiHAMSAPE.
OrthoDBiEOG091G0S7S.
TreeFamiTF300808.

Enzyme and pathway databases

ReactomeiR-BTA-1660499. Synthesis of PIPs at the plasma membrane.
R-BTA-1660514. Synthesis of PIPs at the Golgi membrane.
R-BTA-2132295. MHC class II antigen presentation.
R-BTA-421837. Clathrin derived vesicle budding.
R-BTA-432720. Lysosome Vesicle Biogenesis.
R-BTA-432722. Golgi Associated Vesicle Biogenesis.
R-BTA-6811438. Intra-Golgi traffic.

Miscellaneous databases

EvolutionaryTraceiP84080.

Gene expression databases

BgeeiENSBTAG00000007725.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF1_BOVIN
AccessioniPrimary (citable) accession number: P84080
Secondary accession number(s): A5D7F1
, P10947, P32889, Q3SZG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.