Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei160 – 1601GTP; via amide nitrogenCombined sourcesCurated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 329GTPCombined sourcesCurated
Nucleotide bindingi126 – 1294GTPCombined sourcesCurated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-1660514. Synthesis of PIPs at the Golgi membrane.
R-BTA-2132295. MHC class II antigen presentation.
R-BTA-421837. Clathrin derived vesicle budding.
R-BTA-432720. Lysosome Vesicle Biogenesis.
R-BTA-432722. Golgi Associated Vesicle Biogenesis.
R-BTA-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:ARF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: Ensembl
  • cytosol Source: AgBase
  • extracellular exosome Source: Ensembl
  • focal adhesion Source: Ensembl
  • Golgi apparatus Source: AgBase
  • neuron projection Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: AgBase
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
  • sarcomere Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi71 – 711Q → L: Mainly GTP-bound form; abolishes interaction with TMED10. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 181180ADP-ribosylation factor 1PRO_0000207377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine; alternateBy similarity
Lipidationi2 – 21N-myristoyl glycine; alternateBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Myristate

Proteomic databases

PaxDbiP84080.
PRIDEiP84080.

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition (By similarity). Interacts with IQSEC1 (PubMed:24058294).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ncs1P621682EBI-449051,EBI-907774From a different organism.

Protein-protein interaction databases

IntActiP84080. 8 interactions.
MINTiMINT-126253.
STRINGi9913.ENSBTAP00000010159.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 74Combined sources
Beta strandi19 – 235Combined sources
Helixi30 – 4011Combined sources
Beta strandi49 – 513Combined sources
Beta strandi53 – 575Combined sources
Beta strandi62 – 676Combined sources
Helixi72 – 743Combined sources
Helixi75 – 817Combined sources
Turni82 – 843Combined sources
Beta strandi86 – 938Combined sources
Helixi97 – 993Combined sources
Helixi100 – 11112Combined sources
Helixi114 – 1163Combined sources
Beta strandi120 – 1267Combined sources
Helixi136 – 1427Combined sources
Helixi145 – 1473Combined sources
Beta strandi153 – 1575Combined sources
Turni160 – 1634Combined sources
Helixi166 – 17611Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R8QX-ray1.86A/B1-181[»]
1R8SX-ray1.46A18-181[»]
1S9DX-ray1.80A18-181[»]
4C0AX-ray3.30C/D/G/H18-181[»]
ProteinModelPortaliP84080.
SMRiP84080. Positions 2-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84080.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84080.
KOiK07937.
OMAiHYFANTQ.
OrthoDBiEOG77WWDV.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Length:181
Mass (Da):20,697
Last modified:January 23, 2007 - v2
Checksum:iAAC773D4A60186B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03275 mRNA. Translation: AAA30361.1.
BC102874 mRNA. Translation: AAI02875.1.
BC140532 mRNA. Translation: AAI40533.1.
PIRiA36167.
RefSeqiNP_788826.1. NM_176653.4.
XP_005208456.1. XM_005208399.3.
XP_005208457.1. XM_005208400.3.
UniGeneiBt.91076.
Bt.927.

Genome annotation databases

EnsembliENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725.
GeneIDi338058.
KEGGibta:338058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03275 mRNA. Translation: AAA30361.1.
BC102874 mRNA. Translation: AAI02875.1.
BC140532 mRNA. Translation: AAI40533.1.
PIRiA36167.
RefSeqiNP_788826.1. NM_176653.4.
XP_005208456.1. XM_005208399.3.
XP_005208457.1. XM_005208400.3.
UniGeneiBt.91076.
Bt.927.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R8QX-ray1.86A/B1-181[»]
1R8SX-ray1.46A18-181[»]
1S9DX-ray1.80A18-181[»]
4C0AX-ray3.30C/D/G/H18-181[»]
ProteinModelPortaliP84080.
SMRiP84080. Positions 2-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP84080. 8 interactions.
MINTiMINT-126253.
STRINGi9913.ENSBTAP00000010159.

Proteomic databases

PaxDbiP84080.
PRIDEiP84080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010159; ENSBTAP00000010159; ENSBTAG00000007725.
GeneIDi338058.
KEGGibta:338058.

Organism-specific databases

CTDi375.

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84080.
KOiK07937.
OMAiHYFANTQ.
OrthoDBiEOG77WWDV.
TreeFamiTF300808.

Enzyme and pathway databases

ReactomeiR-BTA-1660514. Synthesis of PIPs at the Golgi membrane.
R-BTA-2132295. MHC class II antigen presentation.
R-BTA-421837. Clathrin derived vesicle budding.
R-BTA-432720. Lysosome Vesicle Biogenesis.
R-BTA-432722. Golgi Associated Vesicle Biogenesis.
R-BTA-6811438. Intra-Golgi traffic.

Miscellaneous databases

EvolutionaryTraceiP84080.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins."
    Sewell J., Kahn R.A.
    Proc. Natl. Acad. Sci. U.S.A. 85:4620-4624(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus and Hereford.
    Tissue: Basal ganglia and Ileum.
  3. "KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET."
    Majoul I., Straub M., Hell S.W., Duden R., Soling H.D.
    Dev. Cell 1:139-153(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TMED2 AND TMED10, MUTAGENESIS OF GLN-71.
  4. "Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor."
    Renault L., Guibert B., Cherfils J.
    Nature 426:525-530(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.46 ANGSTROMS) IN COMPLEX WITH PSCD2; GDP AND BREFELDIN.
  5. "Integrated conformational and lipid-sensing regulation of endosomal ArfGEF BRAG2."
    Aizel K., Biou V., Navaza J., Duarte L.V., Campanacci V., Cherfils J., Zeghouf M.
    PLoS Biol. 11:E1001652-E1001652(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.30 ANGSTROMS) OF 18-181 IN COMPLEX WITH GTP ANALOG AND IQSEC1, INTERACTION WITH IQSEC1.

Entry informationi

Entry nameiARF1_BOVIN
AccessioniPrimary (citable) accession number: P84080
Secondary accession number(s): A5D7F1
, P10947, P32889, Q3SZG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.