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Protein

ADP-ribosylation factor 1

Gene

Arf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160GTP; via amide nitrogenCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 32GTPCombined sources9
Nucleotide bindingi126 – 129GTPCombined sources4

GO - Molecular functioni

  • GDP binding Source: RGD
  • GTP binding Source: RGD
  • magnesium ion binding Source: RGD
  • phospholipase D activator activity Source: RGD

GO - Biological processi

  • actin filament organization Source: RGD
  • dendritic spine organization Source: UniProtKB
  • Golgi to transport vesicle transport Source: RGD
  • long term synaptic depression Source: UniProtKB
  • lysosomal membrane organization Source: RGD
  • mitotic cleavage furrow ingression Source: Ensembl
  • positive regulation of calcium ion-dependent exocytosis Source: RGD
  • positive regulation of dendritic spine development Source: RGD
  • positive regulation of endocytosis Source: RGD
  • positive regulation of ER to Golgi vesicle-mediated transport Source: RGD
  • positive regulation of late endosome to lysosome transport Source: RGD
  • positive regulation of protein secretion Source: RGD
  • positive regulation of sodium ion transmembrane transport Source: RGD
  • protein transport Source: UniProtKB-KW
  • regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
  • regulation of phospholipid metabolic process Source: RGD
  • regulation of receptor internalization Source: UniProtKB
  • synaptic vesicle budding Source: RGD
  • very-low-density lipoprotein particle assembly Source: RGD

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1660499 Synthesis of PIPs at the plasma membrane
R-RNO-1660514 Synthesis of PIPs at the Golgi membrane
R-RNO-2132295 MHC class II antigen presentation
R-RNO-421837 Clathrin derived vesicle budding
R-RNO-432720 Lysosome Vesicle Biogenesis
R-RNO-432722 Golgi Associated Vesicle Biogenesis
R-RNO-6807878 COPI-mediated anterograde transport
R-RNO-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-RNO-6811438 Intra-Golgi traffic

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:Arf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621270 Arf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002073812 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine; alternateBy similarity1
Lipidationi2N-myristoyl glycine; alternateBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Myristate

Proteomic databases

PaxDbiP84079
PRIDEiP84079

2D gel databases

World-2DPAGEi0004:P84079

PTM databases

iPTMnetiP84079
PhosphoSitePlusiP84079
SwissPalmiP84079

Expressioni

Gene expression databases

BgeeiENSRNOG00000060229
GenevisibleiP84079 RN

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition. Interacts with IQSEC1 (By similarity). Interacts with C9orf72 (By similarity).By similarity6 Publications

Protein-protein interaction databases

CORUMiP84079
IntActiP84079, 2 interactors
STRINGi10116.ENSRNOP00000064960

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 10Combined sources8
Beta strandi14 – 16Combined sources3
Beta strandi18 – 25Combined sources8
Helixi30 – 37Combined sources8
Beta strandi42 – 48Combined sources7
Beta strandi51 – 57Combined sources7
Beta strandi59 – 67Combined sources9
Helixi77 – 82Combined sources6
Beta strandi85 – 93Combined sources9
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Beta strandi130 – 132Combined sources3
Helixi136 – 142Combined sources7
Helixi145 – 147Combined sources3
Beta strandi155 – 157Combined sources3
Turni160 – 162Combined sources3
Helixi166 – 178Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RRFX-ray3.00A1-181[»]
1RRGX-ray2.40A/B1-181[»]
ProteinModelPortaliP84079
SMRiP84079
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84079

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP84079
KOiK07937
OMAiHYYSNTD
OrthoDBiEOG091G0S7S
PhylomeDBiP84079
TreeFamiTF300808

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Length:181
Mass (Da):20,697
Last modified:January 23, 2007 - v2
Checksum:iAAC773D4A60186B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12380 mRNA Translation: AAA40685.1
BC061552 mRNA Translation: AAH61552.1
RefSeqiNP_071963.1, NM_022518.3
XP_006246587.1, XM_006246525.3
UniGeneiRn.93735

Genome annotation databases

EnsembliENSRNOT00000080028; ENSRNOP00000072575; ENSRNOG00000060229
GeneIDi64310
KEGGirno:64310
UCSCiRGD:621270 rat

Similar proteinsi

Entry informationi

Entry nameiARF1_RAT
AccessioniPrimary (citable) accession number: P84079
Secondary accession number(s): P10947, P32889
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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