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Protein

ADP-ribosylation factor 1

Gene

Arf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160GTP; via amide nitrogenCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 32GTPCombined sources9
Nucleotide bindingi126 – 129GTPCombined sources4

GO - Molecular functioni

  • GDP binding Source: RGD
  • GTP binding Source: RGD
  • magnesium ion binding Source: RGD
  • phospholipase D activator activity Source: RGD
  • poly(A) RNA binding Source: Ensembl

GO - Biological processi

  • actin filament organization Source: RGD
  • cellular copper ion homeostasis Source: Ensembl
  • dendritic spine organization Source: UniProtKB
  • Golgi to transport vesicle transport Source: RGD
  • long term synaptic depression Source: UniProtKB
  • lysosomal membrane organization Source: RGD
  • positive regulation of calcium ion-dependent exocytosis Source: RGD
  • positive regulation of dendritic spine development Source: RGD
  • positive regulation of endocytosis Source: RGD
  • positive regulation of ER to Golgi vesicle-mediated transport Source: RGD
  • positive regulation of late endosome to lysosome transport Source: RGD
  • positive regulation of protein secretion Source: RGD
  • positive regulation of sodium ion transmembrane transport Source: RGD
  • protein transport Source: UniProtKB-KW
  • regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
  • regulation of phospholipid metabolic process Source: RGD
  • regulation of receptor internalization Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
  • synaptic vesicle budding Source: RGD
  • very-low-density lipoprotein particle assembly Source: RGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1660499. Synthesis of PIPs at the plasma membrane.
R-RNO-1660514. Synthesis of PIPs at the Golgi membrane.
R-RNO-2132295. MHC class II antigen presentation.
R-RNO-421837. Clathrin derived vesicle budding.
R-RNO-432720. Lysosome Vesicle Biogenesis.
R-RNO-432722. Golgi Associated Vesicle Biogenesis.
R-RNO-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:Arf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621270. Arf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002073812 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine; alternateBy similarity1
Lipidationi2N-myristoyl glycine; alternateBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Myristate

Proteomic databases

PaxDbiP84079.
PRIDEiP84079.

2D gel databases

World-2DPAGE0004:P84079.

PTM databases

iPTMnetiP84079.
PhosphoSitePlusiP84079.
SwissPalmiP84079.

Expressioni

Gene expression databases

BgeeiENSRNOG00000030591.
GenevisibleiP84079. RN.

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition. Interacts with IQSEC1 (By similarity).By similarity6 Publications

Protein-protein interaction databases

IntActiP84079. 2 interactors.
STRINGi10116.ENSRNOP00000064960.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 10Combined sources8
Beta strandi14 – 16Combined sources3
Beta strandi18 – 25Combined sources8
Helixi30 – 37Combined sources8
Beta strandi42 – 48Combined sources7
Beta strandi51 – 57Combined sources7
Beta strandi59 – 67Combined sources9
Helixi77 – 82Combined sources6
Beta strandi85 – 93Combined sources9
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Beta strandi130 – 132Combined sources3
Helixi136 – 142Combined sources7
Helixi145 – 147Combined sources3
Beta strandi155 – 157Combined sources3
Turni160 – 162Combined sources3
Helixi166 – 178Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RRFX-ray3.00A1-181[»]
1RRGX-ray2.40A/B1-181[»]
ProteinModelPortaliP84079.
SMRiP84079.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84079.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84079.
KOiK07937.
OMAiALRHRNW.
OrthoDBiEOG091G0S7S.
PhylomeDBiP84079.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Length:181
Mass (Da):20,697
Last modified:January 23, 2007 - v2
Checksum:iAAC773D4A60186B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12380 mRNA. Translation: AAA40685.1.
BC061552 mRNA. Translation: AAH61552.1.
RefSeqiNP_071963.1. NM_022518.3.
XP_006246587.1. XM_006246525.3.
UniGeneiRn.93735.

Genome annotation databases

EnsembliENSRNOT00000080028; ENSRNOP00000072575; ENSRNOG00000060229.
GeneIDi64310.
KEGGirno:64310.
UCSCiRGD:621270. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12380 mRNA. Translation: AAA40685.1.
BC061552 mRNA. Translation: AAH61552.1.
RefSeqiNP_071963.1. NM_022518.3.
XP_006246587.1. XM_006246525.3.
UniGeneiRn.93735.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RRFX-ray3.00A1-181[»]
1RRGX-ray2.40A/B1-181[»]
ProteinModelPortaliP84079.
SMRiP84079.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP84079. 2 interactors.
STRINGi10116.ENSRNOP00000064960.

PTM databases

iPTMnetiP84079.
PhosphoSitePlusiP84079.
SwissPalmiP84079.

2D gel databases

World-2DPAGE0004:P84079.

Proteomic databases

PaxDbiP84079.
PRIDEiP84079.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000080028; ENSRNOP00000072575; ENSRNOG00000060229.
GeneIDi64310.
KEGGirno:64310.
UCSCiRGD:621270. rat.

Organism-specific databases

CTDi375.
RGDi621270. Arf1.

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84079.
KOiK07937.
OMAiALRHRNW.
OrthoDBiEOG091G0S7S.
PhylomeDBiP84079.
TreeFamiTF300808.

Enzyme and pathway databases

ReactomeiR-RNO-1660499. Synthesis of PIPs at the plasma membrane.
R-RNO-1660514. Synthesis of PIPs at the Golgi membrane.
R-RNO-2132295. MHC class II antigen presentation.
R-RNO-421837. Clathrin derived vesicle budding.
R-RNO-432720. Lysosome Vesicle Biogenesis.
R-RNO-432722. Golgi Associated Vesicle Biogenesis.
R-RNO-6811438. Intra-Golgi traffic.

Miscellaneous databases

EvolutionaryTraceiP84079.
PROiP84079.

Gene expression databases

BgeeiENSRNOG00000030591.
GenevisibleiP84079. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF1_RAT
AccessioniPrimary (citable) accession number: P84079
Secondary accession number(s): P10947, P32889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.