P84078 (ARF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosylation factor 1

Gene names

Name:

Arf1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
Subunit structure	Interacts with PRKCABP, PSCD2, ASAP2, HERC1 and PIP5K1B By similarity. Interacts with GGA1 and ARHGAP21. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with TMED2 and TMED10 By similarity.
Subcellular location	Golgi apparatus. Cytoplasm › perinuclear region By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein transport Traceable author statement Ref.1. Source: MGI small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from direct assay. Source: MGI
Cellular component	Golgi apparatus Inferred from direct assay Ref.1. Source: MGI cytosol Inferred from direct assay. Source: MGI endomembrane system Inferred from direct assay. Source: MGI perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell sarcomere Inferred from direct assay. Source: MGI
Molecular function	GTP binding Traceable author statement Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 181

180

ADP-ribosylation factor 1

PRO_0000207380

Regions

Nucleotide binding

24 – 31

8

GTP

Nucleotide binding

126 – 129

4

GTP

Amino acid modifications

Modified residue

147

1

Phosphoserine By similarity

Lipidation

2

1

N-myristoyl glycine By similarity

Experimental info

Sequence conflict

168

1

E → K in BAE31170. Ref.2

Sequence conflict

174

1

S → C in BAE28685. Ref.2

Sequence conflict

180

1

Q → R in BAE40054. Ref.2

Secondary structure

1

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181

Helix Strand Turn

Details...

Beta strand

18 – 23

6

Helix

30 – 39

10

Beta strand

49 – 58

10

Beta strand

61 – 68

8

Helix

72 – 81

10

Beta strand

87 – 93

7

Helix

97 – 99

3

Helix

100 – 111

12

Helix

114 – 116

3

Beta strand

120 – 126

7

Helix

136 – 143

8

Helix

145 – 147

3

Beta strand

153 – 157

5

Turn

160 – 163

4

Helix

166 – 177

12

Sequences

	Sequence		Length	Mass (Da)
	P84078 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AAC773D4A60186B6	FASTA	181	20,697
	10 20 30 40 50 60 MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN 70 80 90 100 110 120 ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV 130 140 150 160 170 180 LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6)." Hosaka M., Toda K., Takatsu H., Torii S., Murakami K., Nakayama K. J. Biochem. 120:813-819(1996) [PubMed: 8947846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: BALB/c, C57BL/6J, DBA/2 and NOD. Tissue: Bone marrow, Heart, Kidney, Pancreas, Placenta and Sympathetic ganglion.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Lubec G., Kang S.U., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 20-30; 39-73; 80-97 AND 110-127, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain and Hippocampus.
[6]	"Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transport." Shiba T., Kawasaki M., Takatsu H., Nogi T., Matsugaki N., Igarashi N., Suzuki M., Kato R., Nakayama K., Wakatsuki S. Nat. Struct. Biol. 10:386-393(2003) [PubMed: 12679809] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 19-182 IN COMPLEX WITH GTP AND GGA1.
[7]	"Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes." Menetrey J., Perderiset M., Cicolari J., Dubois T., Elkhatib N., El Khadali F., Franco M., Chavrier P., Houdusse A. EMBO J. 26:1953-1962(2007) [PubMed: 17347647] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 18-180 IN COMPLEX WITH ARHGAP21.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D87898 mRNA. Translation: BAA13490.1.
AK148458 mRNA. Translation: BAE28566.1.
AK148873 mRNA. Translation: BAE28685.1.
AK150092 mRNA. Translation: BAE29300.1.
AK150699 mRNA. Translation: BAE29778.1.
AK151223 mRNA. Translation: BAE30215.1.
AK151225 mRNA. Translation: BAE30217.1.
AK151696 mRNA. Translation: BAE30620.1.
AK151821 mRNA. Translation: BAE30718.1.
AK152015 mRNA. Translation: BAE30877.1.
AK152382 mRNA. Translation: BAE31170.1.
AK152479 mRNA. Translation: BAE31253.1.
AK153132 mRNA. Translation: BAE31745.1.
AK159174 mRNA. Translation: BAE34874.1.
AK167000 mRNA. Translation: BAE39179.1.
AK167129 mRNA. Translation: BAE39276.1.
AK167543 mRNA. Translation: BAE39610.1.
AK167931 mRNA. Translation: BAE39935.1.
AK168081 mRNA. Translation: BAE40054.1.
AK168686 mRNA. Translation: BAE40532.1.
AK169166 mRNA. Translation: BAE40945.1.
AK170586 mRNA. Translation: BAE41895.1.
AL645854 Genomic DNA. Translation: CAM17105.1.
BC031986 mRNA. Translation: AAH31986.1.

IPI

IPI00221613.

PIR

JC4945.

RefSeq

NP_001123880.1. NM_001130408.1.
NP_031502.1. NM_007476.3.

UniGene

Mm.371546.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O3Y	X-ray	1.50	A/B	18-181	[»]
2J59	X-ray	2.10	A/B/C/D/E/F	18-181	[»]

ProteinModelPortal

P84078.

SMR

P84078. Positions 2-180.

ModBase

Search...

Protein-protein interaction databases

IntAct

P84078. 1 interaction.

MINT

MINT-1862213.

STRING

P84078.

PTM databases

PhosphoSite

P84078.

Proteomic databases

PRIDE

P84078.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000061242; ENSMUSP00000079905; ENSMUSG00000048076.
ENSMUST00000163300; ENSMUSP00000126120; ENSMUSG00000048076.

GeneID

11840.

KEGG

mmu:11840.

UCSC

uc007jdm.2. mouse.

Organism-specific databases

CTD

375.

MGI

MGI:99431. Arf1.

Phylogenomic databases

eggNOG

roNOG06163.

GeneTree

ENSGT00570000078761.

HOVERGEN

HBG002073.

InParanoid

P84078.

OMA

NDRDRMR.

OrthoDB

EOG4PZJ7Q.

PhylomeDB

P84078.

Gene expression databases

ArrayExpress

P84078.

Bgee

P84078.

CleanEx

MM_ARF1.

Genevestigator

P84078.

GermOnline

ENSMUSG00000048076. Mus musculus.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]

KO

K07937.

PANTHER

PTHR11711. ARF/SAR. 1 hit.

Pfam

PF00025. Arf. 1 hit.
[Graphical view]

PRINTS

PR00328. SAR1GTPBP.

SMART

SM00177. ARF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. Small_GTP. 1 hit.

PROSITE

PS51417. ARF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

279783.

SOURCE

Search...

Entry information

Entry name

ARF1_MOUSE

Accession

Primary (citable) accession number: P84078
Secondary accession number(s): P10947 , P32889, Q3THZ2, Q3U849, Q3UDG1, Q3UF76

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program