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Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160GTP; via amide nitrogenCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 32GTPCombined sourcesCurated9
Nucleotide bindingi126 – 129GTPCombined sourcesCurated4

GO - Molecular functioni

  • GDP binding Source: Ensembl
  • GTPase activity Source: Reactome
  • GTP binding Source: UniProtKB-KW
  • magnesium ion binding Source: Ensembl
  • phospholipase D activator activity Source: Ensembl
  • poly(A) RNA binding Source: UniProtKB
  • signal transducer activity, downstream of receptor Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143761-MONOMER.
ReactomeiR-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-1660514. Synthesis of PIPs at the Golgi membrane.
R-HSA-167590. Nef Mediated CD4 Down-regulation.
R-HSA-2132295. MHC class II antigen presentation.
R-HSA-421837. Clathrin derived vesicle budding.
R-HSA-432720. Lysosome Vesicle Biogenesis.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:ARF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:652. ARF1.

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: UniProtKB
  • COPI-coated vesicle Source: Ensembl
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • Golgi membrane Source: Reactome
  • late endosome Source: Ensembl
  • neuron projection Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • peroxisomal membrane Source: Ensembl
  • plasma membrane Source: ProtInc
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
  • sarcomere Source: Ensembl
  • trans-Golgi network Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi375.
OpenTargetsiENSG00000143761.
PharmGKBiPA24934.

Chemistry databases

ChEMBLiCHEMBL5985.

Polymorphism and mutation databases

BioMutaiARF1.
DMDMi51316985.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00002073782 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine; alternateCombined sources1
Lipidationi2N-myristoyl glycine; alternate4 Publications1

Post-translational modificationi

Demyristoylated by S.flexneri cysteine protease IpaJ which cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3.2 Publications

Keywords - PTMi

Acetylation, Lipoprotein, Myristate

Proteomic databases

EPDiP84077.
MaxQBiP84077.
PaxDbiP84077.
PeptideAtlasiP84077.
PRIDEiP84077.
TopDownProteomicsiP84077.

PTM databases

iPTMnetiP84077.
PhosphoSitePlusiP84077.
SwissPalmiP84077.

Expressioni

Gene expression databases

BgeeiENSG00000143761.
CleanExiHS_ARF1.
ExpressionAtlasiP84077. baseline and differential.
GenevisibleiP84077. HS.

Organism-specific databases

HPAiCAB007742.

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition. Interacts with IQSEC1 (PubMed:24058294).9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CYTH2Q994184EBI-447171,EBI-448974
SPAG9O602713EBI-447171,EBI-1023301

Protein-protein interaction databases

BioGridi106870. 80 interactors.
DIPiDIP-31597N.
IntActiP84077. 28 interactors.
MINTiMINT-4999599.
STRINGi9606.ENSP00000272102.

Chemistry databases

BindingDBiP84077.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 9Combined sources4
Beta strandi10 – 12Combined sources3
Beta strandi14 – 16Combined sources3
Beta strandi19 – 25Combined sources7
Helixi30 – 36Combined sources7
Beta strandi42 – 45Combined sources4
Beta strandi49 – 51Combined sources3
Beta strandi53 – 58Combined sources6
Beta strandi61 – 67Combined sources7
Helixi72 – 78Combined sources7
Helixi79 – 82Combined sources4
Beta strandi85 – 93Combined sources9
Beta strandi97 – 99Combined sources3
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Beta strandi131 – 134Combined sources4
Helixi136 – 143Combined sources8
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 163Combined sources4
Helixi166 – 179Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HURX-ray2.00A/B2-181[»]
1RE0X-ray2.40A18-181[»]
1U81NMR-A18-181[»]
3O47X-ray2.80A/B11-181[»]
4HMYX-ray7.00C17-181[»]
ProteinModelPortaliP84077.
SMRiP84077.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84077.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84077.
KOiK07937.
OMAiALRHRNW.
OrthoDBiEOG091G0S7S.
PhylomeDBiP84077.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Length:181
Mass (Da):20,697
Last modified:January 23, 2007 - v2
Checksum:iAAC773D4A60186B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36340 mRNA. Translation: AAA35552.1.
M84326 mRNA. Translation: AAA35512.1.
AF052179 mRNA. Translation: AAC28623.1.
AF055002 mRNA. Translation: AAC09356.1.
AF493881 mRNA. Translation: AAM12595.1.
BT007393 mRNA. Translation: AAP36057.1.
AL136379 Genomic DNA. Translation: CAI23120.1.
BC009247 mRNA. Translation: AAH09247.1.
BC010429 mRNA. Translation: AAH10429.1.
BC011358 mRNA. Translation: AAH11358.1.
M84332 Genomic DNA. Translation: AAA35511.1.
CCDSiCCDS1565.1.
PIRiB40187. A33283.
RefSeqiNP_001019397.1. NM_001024226.1.
NP_001019398.1. NM_001024227.1.
NP_001019399.1. NM_001024228.1.
NP_001649.1. NM_001658.3.
UniGeneiHs.286221.

Genome annotation databases

EnsembliENST00000272102; ENSP00000272102; ENSG00000143761.
ENST00000540651; ENSP00000442980; ENSG00000143761.
ENST00000541182; ENSP00000440005; ENSG00000143761.
GeneIDi375.
KEGGihsa:375.
UCSCiuc001hrr.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36340 mRNA. Translation: AAA35552.1.
M84326 mRNA. Translation: AAA35512.1.
AF052179 mRNA. Translation: AAC28623.1.
AF055002 mRNA. Translation: AAC09356.1.
AF493881 mRNA. Translation: AAM12595.1.
BT007393 mRNA. Translation: AAP36057.1.
AL136379 Genomic DNA. Translation: CAI23120.1.
BC009247 mRNA. Translation: AAH09247.1.
BC010429 mRNA. Translation: AAH10429.1.
BC011358 mRNA. Translation: AAH11358.1.
M84332 Genomic DNA. Translation: AAA35511.1.
CCDSiCCDS1565.1.
PIRiB40187. A33283.
RefSeqiNP_001019397.1. NM_001024226.1.
NP_001019398.1. NM_001024227.1.
NP_001019399.1. NM_001024228.1.
NP_001649.1. NM_001658.3.
UniGeneiHs.286221.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HURX-ray2.00A/B2-181[»]
1RE0X-ray2.40A18-181[»]
1U81NMR-A18-181[»]
3O47X-ray2.80A/B11-181[»]
4HMYX-ray7.00C17-181[»]
ProteinModelPortaliP84077.
SMRiP84077.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106870. 80 interactors.
DIPiDIP-31597N.
IntActiP84077. 28 interactors.
MINTiMINT-4999599.
STRINGi9606.ENSP00000272102.

Chemistry databases

BindingDBiP84077.
ChEMBLiCHEMBL5985.

PTM databases

iPTMnetiP84077.
PhosphoSitePlusiP84077.
SwissPalmiP84077.

Polymorphism and mutation databases

BioMutaiARF1.
DMDMi51316985.

Proteomic databases

EPDiP84077.
MaxQBiP84077.
PaxDbiP84077.
PeptideAtlasiP84077.
PRIDEiP84077.
TopDownProteomicsiP84077.

Protocols and materials databases

DNASUi375.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000272102; ENSP00000272102; ENSG00000143761.
ENST00000540651; ENSP00000442980; ENSG00000143761.
ENST00000541182; ENSP00000440005; ENSG00000143761.
GeneIDi375.
KEGGihsa:375.
UCSCiuc001hrr.4. human.

Organism-specific databases

CTDi375.
DisGeNETi375.
GeneCardsiARF1.
H-InvDBHIX0116279.
HGNCiHGNC:652. ARF1.
HPAiCAB007742.
MIMi103180. gene.
neXtProtiNX_P84077.
OpenTargetsiENSG00000143761.
PharmGKBiPA24934.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP84077.
KOiK07937.
OMAiALRHRNW.
OrthoDBiEOG091G0S7S.
PhylomeDBiP84077.
TreeFamiTF300808.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143761-MONOMER.
ReactomeiR-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-1660514. Synthesis of PIPs at the Golgi membrane.
R-HSA-167590. Nef Mediated CD4 Down-regulation.
R-HSA-2132295. MHC class II antigen presentation.
R-HSA-421837. Clathrin derived vesicle budding.
R-HSA-432720. Lysosome Vesicle Biogenesis.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-6811438. Intra-Golgi traffic.

Miscellaneous databases

ChiTaRSiARF1. human.
EvolutionaryTraceiP84077.
GeneWikiiARF1.
GenomeRNAii375.
PROiP84077.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143761.
CleanExiHS_ARF1.
ExpressionAtlasiP84077. baseline and differential.
GenevisibleiP84077. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF1_HUMAN
AccessioniPrimary (citable) accession number: P84077
Secondary accession number(s): P10947, P32889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.