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Protein

Neuron-specific calcium-binding protein hippocalcin

Gene

Hpca

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding protein that may play a role in the regulation of voltage-dependent calcium channels (By similarity). May also play a role in cyclic-nucleotide-mediated signaling through the regulation of adenylate and guanylate cyclases (PubMed:15336960).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi73 – 841By similarityAdd BLAST12
Calcium bindingi109 – 1202By similarityAdd BLAST12
Calcium bindingi157 – 1683By similarityAdd BLAST12

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • kinase binding Source: RGD

GO - Biological processi

  • activation of phospholipase D activity Source: RGD
  • brain development Source: RGD
  • calcium-mediated signaling Source: RGD
  • cellular response to calcium ion Source: UniProtKB
  • cellular response to electrical stimulus Source: RGD
  • cellular response to monosodium glutamate Source: RGD
  • inner ear development Source: Ensembl
  • negative regulation of guanylate cyclase activity Source: RGD
  • positive regulation of adenylate cyclase activity Source: RGD
  • positive regulation of phospholipase activity Source: RGD
  • positive regulation of protein targeting to membrane Source: RGD
  • regulation of voltage-gated calcium channel activity Source: UniProtKB
  • response to Aroclor 1254 Source: RGD
  • response to ketamine Source: RGD
  • response to L-glutamate Source: RGD
  • response to organic cyclic compound Source: RGD
  • retina development in camera-type eye Source: RGD

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuron-specific calcium-binding protein hippocalcinCurated
Alternative name(s):
P23K1 Publication
Gene namesi
Name:HpcaImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620060. Hpca.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000737702 – 193Neuron-specific calcium-binding protein hippocalcinAdd BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Post-translational modificationi

Myristoylation facilitates association with membranes.1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP84076.
PRIDEiP84076.

PTM databases

iPTMnetiP84076.
PhosphoSitePlusiP84076.

Expressioni

Tissue specificityi

Neuron-specific in the hippocampus (PubMed:1280427). Also detected in olfactory eptihelium (PubMed:15336960).2 Publications

Gene expression databases

BgeeiENSRNOG00000006979.
GenevisibleiP84076. RN.

Interactioni

Subunit structurei

Oligomer; oligomerization is calcium-dependent. May interact with the voltage-dependent P/Q- and N-type calcium channels CACNA1A and CACNA1B.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE1AP141002EBI-908193,EBI-907809From Bos taurus.

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • kinase binding Source: RGD

Protein-protein interaction databases

CORUMiP84076.
IntActiP84076. 3 interactors.
STRINGi10116.ENSRNOP00000009153.

Structurei

3D structure databases

ProteinModelPortaliP84076.
SMRiP84076.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 59EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini60 – 95EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini96 – 131EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini144 – 179EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Domaini

Binds 3 calcium via EF-hand domains. The cryptic EF-hand 1 does not bind calcium.2 Publications

Sequence similaritiesi

Belongs to the recoverin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP84076.
OMAiRTFDTNN.
OrthoDBiEOG091G11T4.
PhylomeDBiP84076.
TreeFamiTF300009.

Family and domain databases

CDDicd00051. EFh. 2 hits.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
PfamiView protein in Pfam
PF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 3 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKQNSKLRP EMLQDLRENT EFSELELQEW YKGFLKDCPT GILNVDEFKK
60 70 80 90 100
IYANFFPYGD ASKFAEHVFR TFDTNSDGTI DFREFIIALS VTSRGRLEQK
110 120 130 140 150
LMWAFSMYDL DGNGYISREE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE
160 170 180 190
KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF
Length:193
Mass (Da):22,427
Last modified:January 23, 2007 - v2
Checksum:i3DBEA176AC945DB1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60D → G AA sequence (PubMed:1543495).Curated1
Sequence conflicti102M → N AA sequence (PubMed:1543495).Curated1
Sequence conflicti113N → D AA sequence (PubMed:1543495).Curated1
Sequence conflicti142 – 143ES → TF AA sequence (PubMed:1543495).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12573 mRNA. Translation: BAA02122.1.
AY442172 mRNA. Translation: AAR14053.1.
BC087632 mRNA. Translation: AAH87632.1.
X96993 Genomic DNA. Translation: CAA65718.1.
PIRiJC1347.
PS0344.
RefSeqiNP_058818.1. NM_017122.1.
XP_006238982.1. XM_006238920.2.
XP_006238983.1. XM_006238921.3.
UniGeneiRn.11019.

Genome annotation databases

EnsembliENSRNOT00000009153; ENSRNOP00000009153; ENSRNOG00000006979.
GeneIDi29177.
KEGGirno:29177.

Similar proteinsi

Entry informationi

Entry nameiHPCA_RAT
AccessioniPrimary (citable) accession number: P84076
Secondary accession number(s): P32076, P41211, P70510
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families