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Reviewed, UniProtKB/Swiss-Prot P84076 (HPCA_RAT)

Last modified November 4, 2008. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Neuron-specific calcium-binding protein hippocalcin
Alternative name(s):
    P23K
Gene names
Name: Hpca
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds two calcium ions.

Tissue specificity

Neuron-specific in the hippocampus.

Post-translational modification

Myristoylation facilitates interaction with membranes.

Sequence similarities

Belongs to the recoverin family.

Contains 4 EF-hand domains.

Ontologies

Keywords

   DomainRepeat
   LigandCalcium
   PTMLipoprotein
Myristate
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Molecular functionactin binding

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion binding Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PDE1AP141001EBI-908193,EBI-907809From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 193192Neuron-specific calcium-binding protein hippocalcin
PRO_0000073770

Regions

Domain24 – 5936EF-hand 1
Domain60 – 9536EF-hand 2
Domain96 – 13136EF-hand 3
Domain144 – 17936EF-hand 4
Calcium binding73 – 84121 Potential
Calcium binding109 – 120122 Potential
Calcium binding157 – 168123 Potential

Amino acid modifications

Lipidation21N-myristoyl glycine

Experimental info

Sequence conflict601D → G AA sequence Ref.5
Sequence conflict1021M → N AA sequence Ref.5
Sequence conflict1131N → D AA sequence Ref.5
Sequence conflict142 – 1432ES → TF AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
P84076-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 3DBEA176AC945DB1

FASTA19322,427
        10         20         30         40         50         60 
MGKQNSKLRP EMLQDLRENT EFSELELQEW YKGFLKDCPT GILNVDEFKK IYANFFPYGD 

        70         80         90        100        110        120 
ASKFAEHVFR TFDTNSDGTI DFREFIIALS VTSRGRLEQK LMWAFSMYDL DGNGYISREE 

       130        140        150        160        170        180 
MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV 

       190 
RLLQCDPSSA SQF 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus."
Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T.
Biochem. Biophys. Res. Commun. 189:511-517(1992) [PubMed: 1280427] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Wistar.
Tissue: Brain.
[2]"Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus."
Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T.
Biochem. Biophys. Res. Commun. 196:1017-1017(1993) [PubMed: 8240319] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Hippocalcin in the olfactory epithelium: a mediator of second messenger signaling."
Mammen A., Simpson P.J., Nighorn A., Imanishi Y., Palczewski K., Ronnett G.V., Moon C.
Biochem. Biophys. Res. Commun. 322:1131-1139(2004) [PubMed: 15336960] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Sprague-Dawley.
Tissue: Olfactory epithelium.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Isolation and characterization of recoverin-like Ca(2+)-binding protein from rat brain."
Takamatsu K., Kitamura K., Noguchi T.
Biochem. Biophys. Res. Commun. 183:245-251(1992) [PubMed: 1543495] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 51-63; 101-116 AND 138-145, CHARACTERIZATION.
Tissue: Brain.
[6]"Characterization of the rat hippocalcin gene: the 5' flanking region directs expression to the hippocampus."
Grant A.L., Jones A., Thomas K.L., Wisden W.
Neuroscience 75:1099-1115(1996) [PubMed: 8938744] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
Strain: Sprague-Dawley.
Tissue: Testis.
[7]Lubec G., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 51-94; 138-148; 164-171 AND 175-181, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[8]"Myristoylation of hippocalcin is linked to its calcium-dependent membrane association properties."
Kobayashi M., Takamatsu K., Saitoh S., Noguchi T.
J. Biol. Chem. 268:18898-18904(1993) [PubMed: 8360179] [Abstract]
Cited for: MYRISTOYLATION AT GLY-2.

Cross-references

Sequence databases

D12573 mRNA. Translation: BAA02122.1.
AY442172 mRNA. Translation: AAR14053.1.
BC087632 mRNA. Translation: AAH87632.1.
X96993 Genomic DNA. Translation: CAA65718.1.
PIRJC1347.
PS0344.
RefSeqNP_058818.1.
UniGeneRn.11019

3D structure databases

HSSPHSSP built from PDB template 1BJF based on UniProtKB P29554.
SMRP84076. Positions 5-185.
ModBaseSearch...

Protein-protein interaction databases

IntActP84076.

PTM databases

PhosphoSiteP84076.

Genome annotation databases

EnsemblENSRNOG00000006979. Rattus norvegicus. [Contig view]
GeneID29177.
KEGGrno:29177.

Organism-specific databases

RGD620060. Hpca.

Phylogenomic databases

HOVERGENP84076.

Gene expression databases

ArrayExpressP84076.
GermOnlineENSRNOG00000006979. Rattus norvegicus.

Family and domain databases

InterProIPR011992. EF-Hand_type.
IPR002048. EF_hand_Ca_bd.
IPR001125. Recoverin.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PfamPF00036. efhand. 3 hits.
[Graphical view]
PRINTSPR00450. RECOVERIN.
ProDomPD000012. EF-hand. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00054. EFh. 3 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio608252.

Entry information

Entry nameHPCA_RAT
AccessionPrimary (citable) accession number: P84076
Secondary accession number(s): P32076, P41211, P70510
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 4, 2008
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents