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Protein

Neuron-specific calcium-binding protein hippocalcin

Gene

Hpca

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds two calcium ions.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi73 – 84121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi109 – 120122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi157 – 168123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • calcium ion binding Source: ProtInc
  • kinase binding Source: RGD

GO - Biological processi

  • activation of phospholipase D activity Source: RGD
  • brain development Source: RGD
  • calcium-mediated signaling Source: RGD
  • cellular response to electrical stimulus Source: RGD
  • cellular response to monosodium glutamate Source: RGD
  • inner ear development Source: Ensembl
  • negative regulation of guanylate cyclase activity Source: RGD
  • positive regulation of adenylate cyclase activity Source: RGD
  • positive regulation of phospholipase activity Source: RGD
  • positive regulation of protein targeting to membrane Source: RGD
  • response to Aroclor 1254 Source: RGD
  • response to ketamine Source: RGD
  • response to L-glutamate Source: RGD
  • response to organic cyclic compound Source: RGD
  • retina development in camera-type eye Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuron-specific calcium-binding protein hippocalcin
Alternative name(s):
P23K
Gene namesi
Name:Hpca
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620060. Hpca.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • dendrite cytoplasm Source: RGD
  • dendrite membrane Source: RGD
  • dendritic spine head Source: RGD
  • neuronal cell body membrane Source: RGD
  • perikaryon Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 193192Neuron-specific calcium-binding protein hippocalcinPRO_0000073770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Post-translational modificationi

Myristoylation facilitates interaction with membranes.1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP84076.
PRIDEiP84076.

PTM databases

iPTMnetiP84076.
PhosphoSiteiP84076.

Expressioni

Tissue specificityi

Neuron-specific in the hippocampus.1 Publication

Gene expression databases

GenevisibleiP84076. RN.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE1AP141002EBI-908193,EBI-907809From a different organism.

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • kinase binding Source: RGD

Protein-protein interaction databases

IntActiP84076. 3 interactions.
STRINGi10116.ENSRNOP00000009153.

Structurei

3D structure databases

ProteinModelPortaliP84076.
SMRiP84076. Positions 5-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 5936EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini60 – 9536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini96 – 13136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini144 – 17936EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP84076.
OMAiERHSSPH.
OrthoDBiEOG7GJ6F3.
PhylomeDBiP84076.
TreeFamiTF300009.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKQNSKLRP EMLQDLRENT EFSELELQEW YKGFLKDCPT GILNVDEFKK
60 70 80 90 100
IYANFFPYGD ASKFAEHVFR TFDTNSDGTI DFREFIIALS VTSRGRLEQK
110 120 130 140 150
LMWAFSMYDL DGNGYISREE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE
160 170 180 190
KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF
Length:193
Mass (Da):22,427
Last modified:January 23, 2007 - v2
Checksum:i3DBEA176AC945DB1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601D → G AA sequence (PubMed:1543495).Curated
Sequence conflicti102 – 1021M → N AA sequence (PubMed:1543495).Curated
Sequence conflicti113 – 1131N → D AA sequence (PubMed:1543495).Curated
Sequence conflicti142 – 1432ES → TF AA sequence (PubMed:1543495).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12573 mRNA. Translation: BAA02122.1.
AY442172 mRNA. Translation: AAR14053.1.
BC087632 mRNA. Translation: AAH87632.1.
X96993 Genomic DNA. Translation: CAA65718.1.
PIRiJC1347.
PS0344.
RefSeqiNP_058818.1. NM_017122.1.
XP_006238982.1. XM_006238920.1.
XP_006238983.1. XM_006238921.2.
UniGeneiRn.11019.

Genome annotation databases

EnsembliENSRNOT00000009153; ENSRNOP00000009153; ENSRNOG00000006979.
GeneIDi29177.
KEGGirno:29177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12573 mRNA. Translation: BAA02122.1.
AY442172 mRNA. Translation: AAR14053.1.
BC087632 mRNA. Translation: AAH87632.1.
X96993 Genomic DNA. Translation: CAA65718.1.
PIRiJC1347.
PS0344.
RefSeqiNP_058818.1. NM_017122.1.
XP_006238982.1. XM_006238920.1.
XP_006238983.1. XM_006238921.2.
UniGeneiRn.11019.

3D structure databases

ProteinModelPortaliP84076.
SMRiP84076. Positions 5-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP84076. 3 interactions.
STRINGi10116.ENSRNOP00000009153.

PTM databases

iPTMnetiP84076.
PhosphoSiteiP84076.

Proteomic databases

PaxDbiP84076.
PRIDEiP84076.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009153; ENSRNOP00000009153; ENSRNOG00000006979.
GeneIDi29177.
KEGGirno:29177.

Organism-specific databases

CTDi3208.
RGDi620060. Hpca.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP84076.
OMAiERHSSPH.
OrthoDBiEOG7GJ6F3.
PhylomeDBiP84076.
TreeFamiTF300009.

Miscellaneous databases

PROiP84076.

Gene expression databases

GenevisibleiP84076. RN.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus."
    Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T.
    Biochem. Biophys. Res. Commun. 189:511-517(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Brain.
  2. "Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus."
    Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T.
    Biochem. Biophys. Res. Commun. 196:1017-1017(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "Hippocalcin in the olfactory epithelium: a mediator of second messenger signaling."
    Mammen A., Simpson P.J., Nighorn A., Imanishi Y., Palczewski K., Ronnett G.V., Moon C.
    Biochem. Biophys. Res. Commun. 322:1131-1139(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Olfactory epithelium.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Isolation and characterization of recoverin-like Ca(2+)-binding protein from rat brain."
    Takamatsu K., Kitamura K., Noguchi T.
    Biochem. Biophys. Res. Commun. 183:245-251(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 51-63; 101-116 AND 138-145, CHARACTERIZATION.
    Tissue: Brain.
  6. "Characterization of the rat hippocalcin gene: the 5' flanking region directs expression to the hippocampus."
    Grant A.L., Jones A., Thomas K.L., Wisden W.
    Neuroscience 75:1099-1115(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
    Strain: Sprague-Dawley.
    Tissue: Testis.
  7. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 51-94; 138-148; 164-171 AND 175-181, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  8. "Myristoylation of hippocalcin is linked to its calcium-dependent membrane association properties."
    Kobayashi M., Takamatsu K., Saitoh S., Noguchi T.
    J. Biol. Chem. 268:18898-18904(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MYRISTOYLATION AT GLY-2.

Entry informationi

Entry nameiHPCA_RAT
AccessioniPrimary (citable) accession number: P84076
Secondary accession number(s): P32076, P41211, P70510
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.