P84076 (HPCA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuron-specific calcium-binding protein hippocalcin

Alternative name(s):
P23K

Gene names

Name:

Hpca

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds two calcium ions.
Tissue specificity	Neuron-specific in the hippocampus. Ref.1
Post-translational modification	Myristoylation facilitates interaction with membranes.
Sequence similarities	Belongs to the recoverin family. Contains 4 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	actin binding Inferred from sequence or structural similarity. Source: UniProtKB calcium ion binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PDE1A	P14100	2	EBI-908193,EBI-907809	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 193	192	Neuron-specific calcium-binding protein hippocalcin		PRO_0000073770
Regions
	Domain	24 – 59	36	EF-hand 1
	Domain	60 – 95	36	EF-hand 2
	Domain	96 – 131	36	EF-hand 3
	Domain	144 – 179	36	EF-hand 4
	Calcium binding	73 – 84	12	1 Potential
	Calcium binding	109 – 120	12	2 Potential
	Calcium binding	157 – 168	12	3 Potential
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Ref.8
Experimental info
	Sequence conflict	60	1	D → G AA sequence Ref.5
	Sequence conflict	102	1	M → N AA sequence Ref.5
	Sequence conflict	113	1	N → D AA sequence Ref.5
	Sequence conflict	142 – 143	2	ES → TF AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P84076 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 3DBEA176AC945DB1	FASTA	193	22,427
	10 20 30 40 50 60 MGKQNSKLRP EMLQDLRENT EFSELELQEW YKGFLKDCPT GILNVDEFKK IYANFFPYGD 70 80 90 100 110 120 ASKFAEHVFR TFDTNSDGTI DFREFIIALS VTSRGRLEQK LMWAFSMYDL DGNGYISREE 130 140 150 160 170 180 MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV 190 RLLQCDPSSA SQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus." Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T. Biochem. Biophys. Res. Commun. 189:511-517(1992) [PubMed: 1280427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Wistar. Tissue: Brain.
[2]	"Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus." Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T. Biochem. Biophys. Res. Commun. 196:1017-1017(1993) [PubMed: 8240319] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Hippocalcin in the olfactory epithelium: a mediator of second messenger signaling." Mammen A., Simpson P.J., Nighorn A., Imanishi Y., Palczewski K., Ronnett G.V., Moon C. Biochem. Biophys. Res. Commun. 322:1131-1139(2004) [PubMed: 15336960] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley. Tissue: Olfactory epithelium.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Isolation and characterization of recoverin-like Ca(2+)-binding protein from rat brain." Takamatsu K., Kitamura K., Noguchi T. Biochem. Biophys. Res. Commun. 183:245-251(1992) [PubMed: 1543495] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 51-63; 101-116 AND 138-145, CHARACTERIZATION. Tissue: Brain.
[6]	"Characterization of the rat hippocalcin gene: the 5' flanking region directs expression to the hippocampus." Grant A.L., Jones A., Thomas K.L., Wisden W. Neuroscience 75:1099-1115(1996) [PubMed: 8938744] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37. Strain: Sprague-Dawley. Tissue: Testis.
[7]	Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 51-94; 138-148; 164-171 AND 175-181, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
[8]	"Myristoylation of hippocalcin is linked to its calcium-dependent membrane association properties." Kobayashi M., Takamatsu K., Saitoh S., Noguchi T. J. Biol. Chem. 268:18898-18904(1993) [PubMed: 8360179] [Abstract] Cited for: MYRISTOYLATION AT GLY-2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12573 mRNA. Translation: BAA02122.1. AY442172 mRNA. Translation: AAR14053.1. BC087632 mRNA. Translation: AAH87632.1. X96993 Genomic DNA. Translation: CAA65718.1.
IPI	IPI00231685.
PIR	JC1347. PS0344.
RefSeq	NP_058818.1. NM_017122.1.
UniGene	Rn.11019.
3D structure databases
ProteinModelPortal	P84076.
SMR	P84076. Positions 5-185.
ModBase	Search...
Protein-protein interaction databases
IntAct	P84076. 3 interactions.
STRING	P84076.
PTM databases
PhosphoSite	P84076.
Proteomic databases
PRIDE	P84076.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000009153; ENSRNOP00000009153; ENSRNOG00000006979.
GeneID	29177.
KEGG	rno:29177.
UCSC	NM_017122. rat.
Organism-specific databases
CTD	3208.
RGD	620060. Hpca.
Phylogenomic databases
eggNOG	maNOG13155.
GeneTree	ENSGT00560000076803.
HOVERGEN	HBG108179.
InParanoid	P84076.
OMA	KEDESTP.
OrthoDB	EOG4G1MHC.
PhylomeDB	P84076.
Gene expression databases
ArrayExpress	P84076.
Genevestigator	P84076.
GermOnline	ENSRNOG00000006979. Rattus norvegicus.
Family and domain databases
InterPro	IPR018248. EF-hand. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR001125. Recoverin. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 3 hits. [Graphical view]
PRINTS	PR00450. RECOVERIN.
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	608252.

Entry information

Entry name

HPCA_RAT

Accession

Primary (citable) accession number: P84076
Secondary accession number(s): P32076, P41211, P70510

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program