P84076 (HPCA_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
September 21, 2011.
Version 65.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Neuron-specific calcium-binding protein hippocalcin Alternative name(s): P23K | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 193 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds two calcium ions. |
| Tissue specificity | Neuron-specific in the hippocampus. Ref.1 |
| Post-translational modification | Myristoylation facilitates interaction with membranes. |
| Sequence similarities | Belongs to the recoverin family. Contains 4 EF-hand domains. |
Ontologies
| Keywords | |
|---|---|
| Domain | Repeat |
| Ligand | Calcium |
| PTM | Lipoprotein Myristate |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Molecular function | actin binding Inferred from sequence or structural similarity. Source: UniProtKB calcium ion bindingTraceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| PDE1A | P14100 | 2 | EBI-908193,EBI-907809 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 193 | 192 | Neuron-specific calcium-binding protein hippocalcin | PRO_0000073770 | |||||
Regions | |||||||||
| Domain | 24 – 59 | 36 | EF-hand 1 | ||||||
| Domain | 60 – 95 | 36 | EF-hand 2 | ||||||
| Domain | 96 – 131 | 36 | EF-hand 3 | ||||||
| Domain | 144 – 179 | 36 | EF-hand 4 | ||||||
| Calcium binding | 73 – 84 | 12 | 1 Potential | ||||||
| Calcium binding | 109 – 120 | 12 | 2 Potential | ||||||
| Calcium binding | 157 – 168 | 12 | 3 Potential | ||||||
Amino acid modifications | |||||||||
| Lipidation | 2 | 1 | N-myristoyl glycine Ref.8 | ||||||
Experimental info | |||||||||
| Sequence conflict | 60 | 1 | D → G AA sequence Ref.5 | ||||||
| Sequence conflict | 102 | 1 | M → N AA sequence Ref.5 | ||||||
| Sequence conflict | 113 | 1 | N → D AA sequence Ref.5 | ||||||
| Sequence conflict | 142 – 143 | 2 | ES → TF AA sequence Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus." Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T. Biochem. Biophys. Res. Commun. 189:511-517(1992) [PubMed: 1280427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Wistar. Tissue: Brain. |
| [2] | "Molecular cloning of hippocalcin, a novel calcium-binding protein of the recoverin family exclusively expressed in hippocampus." Kobayashi M., Takamatsu K., Saitoh S., Miura M., Noguchi T. Biochem. Biophys. Res. Commun. 196:1017-1017(1993) [PubMed: 8240319] [Abstract] Cited for: SEQUENCE REVISION. |
| [3] | "Hippocalcin in the olfactory epithelium: a mediator of second messenger signaling." Mammen A., Simpson P.J., Nighorn A., Imanishi Y., Palczewski K., Ronnett G.V., Moon C. Biochem. Biophys. Res. Commun. 322:1131-1139(2004) [PubMed: 15336960] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley. Tissue: Olfactory epithelium. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [5] | "Isolation and characterization of recoverin-like Ca(2+)-binding protein from rat brain." Takamatsu K., Kitamura K., Noguchi T. Biochem. Biophys. Res. Commun. 183:245-251(1992) [PubMed: 1543495] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 51-63; 101-116 AND 138-145, CHARACTERIZATION. Tissue: Brain. |
| [6] | "Characterization of the rat hippocalcin gene: the 5' flanking region directs expression to the hippocampus." Grant A.L., Jones A., Thomas K.L., Wisden W. Neuroscience 75:1099-1115(1996) [PubMed: 8938744] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37. Strain: Sprague-Dawley. Tissue: Testis. |
| [7] | Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 51-94; 138-148; 164-171 AND 175-181, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain. |
| [8] | "Myristoylation of hippocalcin is linked to its calcium-dependent membrane association properties." Kobayashi M., Takamatsu K., Saitoh S., Noguchi T. J. Biol. Chem. 268:18898-18904(1993) [PubMed: 8360179] [Abstract] Cited for: MYRISTOYLATION AT GLY-2. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D12573 mRNA. Translation: BAA02122.1. AY442172 mRNA. Translation: AAR14053.1. BC087632 mRNA. Translation: AAH87632.1. X96993 Genomic DNA. Translation: CAA65718.1. |
| IPI | IPI00231685. |
| PIR | JC1347. PS0344. |
| RefSeq | NP_058818.1. NM_017122.1. |
| UniGene | Rn.11019. |
3D structure databases | |
| ProteinModelPortal | P84076. |
| SMR | P84076. Positions 5-185. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P84076. 3 interactions. |
| STRING | P84076. |
PTM databases | |
| PhosphoSite | P84076. |
Proteomic databases | |
| PRIDE | P84076. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOT00000009153; ENSRNOP00000009153; ENSRNOG00000006979. |
| GeneID | 29177. |
| KEGG | rno:29177. |
| UCSC | NM_017122. rat. |
Organism-specific databases | |
| CTD | 3208. |
| RGD | 620060. Hpca. |
Phylogenomic databases | |
| eggNOG | maNOG13155. |
| GeneTree | ENSGT00560000076803. |
| HOVERGEN | HBG108179. |
| InParanoid | P84076. |
| OMA | KEDESTP. |
| OrthoDB | EOG4G1MHC. |
| PhylomeDB | P84076. |
Gene expression databases | |
| ArrayExpress | P84076. |
| Genevestigator | P84076. |
| GermOnline | ENSRNOG00000006979. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR018248. EF-hand. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR001125. Recoverin. [Graphical view] |
| Gene3D | G3DSA:1.10.238.10. EF-Hand_type. 1 hit. |
| Pfam | PF00036. efhand. 3 hits. [Graphical view] |
| PRINTS | PR00450. RECOVERIN. |
| SMART | SM00054. EFh. 3 hits. [Graphical view] |
| PROSITE | PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 3 hits. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 608252. |
Entry information
| Entry name | HPCA_RAT | ||||||||
| Accession | Primary (citable) accession number: P84076 Secondary accession number(s): P32076, P41211, P70510 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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