Reviewed, UniProtKB/Swiss-Prot P84073 (RIPL_LUFCY)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein luffacylin     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Luffa cylindrica (Smooth loofah) (Sponge gourd)
Taxonomic identifier	3670 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Luffa

Protein attributes

Sequence length	14 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has antifungal activity against F.oxysporum and M.arachidicola. Inhibits cell-free translation in rabbit reticulocyte lysate system. Has some N-glycosidase activity. Ref.1
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.1

Ontologies

Keywords
Molecular function	Antimicrobial Fungicide Glycosidase Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of another organism Inferred from electronic annotation. Source: UniProtKB-KW metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›14	›14	Ribosome-inactivating protein luffacylin		PRO_0000221425
Experimental info
	Non-terminal residue	14	1

Sequences

	Sequence		Length	Mass (Da)
	P84073-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 87CB5B5507D44B01	FASTA	14	1,646
	10 PRGSPRTEYE AARR

References

[1]	"Isolation and characterization of luffacylin, a ribosome inactivating peptide with anti-fungal activity from sponge gourd (Luffa cylindrica) seeds." Parkash A., Ng T.B., Tso W.W. Peptides 23:1019-1024(2002) [PubMed: 12126727] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION. Tissue: Seed.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273560.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

RIPL_LUFCY

Accession

Primary (citable) accession number: P84073

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 19, 2004
Last modified:	October 13, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)