Reviewed, UniProtKB/Swiss-Prot P84072 (CHAR_MOMCH)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein charantin     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Momordica charantia (Bitter gourd) (Balsam pear)
Taxonomic identifier	3673 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Momordica

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits cell-free translation in a rabbit reticulocyte lysate system. Ref.1
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.1
Subunit structure	Monomer Probable.
Miscellaneous	IC50 of translation-inhibiting activity is of 400 nM.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	Ribosome-inactivating protein charantin		PRO_0000221424
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	P84072-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: BFEBA068800E0696	FASTA	25	2,905
	10 20 LRNVEQQCRA DALVERAQEL IHGQQ

References

[1]	"Purification and characterization of charantin, a napin-like ribosome-inactivating peptide from bitter gourd (Momordica charantia) seeds." Parkash A., Ng T.B., Tso W.W. J. Pept. Res. 59:197-202(2002) [PubMed: 11966976] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION. Tissue: Seed.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273563.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

CHAR_MOMCH

Accession

Primary (citable) accession number: P84072

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)