Reviewed, UniProtKB/Swiss-Prot P84029 (CYC2_DROME)

Names and origin

Protein names

Recommended name:
    Cytochrome c-2
Alternative name(s):
    Cytochrome c-proximal

Gene names

Name:	Cyt-c-p
Synonyms:	CYTC2, DC4
ORF Names:	CG17903

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Ref.1
Subcellular location	Mitochondrion matrix. Ref.2
Developmental stage	Expressed at varying, but relatively high levels throughout development. Ref.1
Post-translational modification	Binds 1 heme group per subunit.
Miscellaneous	There are two cytochrome C genes in Drosophila: Cyt-c-d (distal) and Cyt-c-p (proximal).
Sequence similarities	Belongs to the cytochrome c family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Ref.1 Inferred from direct assay. Source: FlyBase heme binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VK57	1	EBI-85896,EBI-170636

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 108	107	Cytochrome c-2		PRO_0000108261
Sites
	Metal binding	23	1	Iron (heme axial ligand)
	Metal binding	85	1	Iron (heme axial ligand)
	Binding site	19	1	Heme (covalent)
	Binding site	22	1	Heme (covalent)

Sequences

	Sequence		Length	Mass (Da)
	P84029-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 535FFBAF282D20D7	FASTA	108	11,735
	10 20 30 40 50 60 MGVPAGDVEK GKKLFVQRCA QCHTVEAGGK HKVGPNLHGL IGRKTGQAAG FAYTDANKAK 70 80 90 100 GITWNEDTLF EYLENPKKYI PGTKMIFAGL KKPNERGDLI AYLKSATK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two Drosophila melanogaster cytochrome c genes and their transcripts." Limbach K.J., Wu R. Nucleic Acids Res. 13:631-644(1985) [PubMed: 2987802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE.
[2]	"Developmental expression of nuclear genes that encode mitochondrial proteins: insect cytochromes c." Swanson M.S., Zieminn S.M., Miller D.D., Garber E.A.E., Margoliash E. Proc. Natl. Acad. Sci. U.S.A. 82:1964-1968(1985) [PubMed: 2984675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[6]	"Developmental variation and amino acid sequences of cytochromes c of the fruit fly Drosophila melanogaster and the flesh fly Boettcherisca peregrina." Inoue S., Inoue H., Hiroyoshi T., Matsubara H., Yamanaka T. J. Biochem. 100:955-965(1986) [PubMed: 3029051] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-108.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Cross-references

Sequence databases
EMBLGenBankDDBJ	X01760 Genomic DNA. Translation: CAA25900.1. M11381 Genomic DNA. Translation: AAA28437.1. AE014134 Genomic DNA. Translation: AAF53554.1. AY071701 mRNA. Translation: AAL49323.1.
PIR	CCFFDM. A22945.
RefSeq	NP_477176.1.
UniGene	Dm.4850
3D structure databases
HSSP	HSSP built from PDB template 2PCB based on UniProtKB P00004.
SMR	P84029. Positions 6-107.
ModBase	Search...
Protein-protein interaction databases
IntAct	P84029. 23 interactions.
Genome annotation databases
Ensembl	FBgn0000409. Drosophila melanogaster. [Contig view]
GeneID	34996.
KEGG	dme:Dmel_CG17903.
NMPDR	fig|7227.3.peg.2658.
Organism-specific databases
FlyBase	FBgn0000409. Cyt-c-p.
Phylogenomic databases
HOGENOM	P84029.
OMA	P84029. IAYLKQY.
Gene expression databases
ArrayExpress	P84029.
GermOnline	CG17903. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002327. Cyt_c_1A/1B. IPR003088. Cyt_c_I. IPR009056. Cyt_c_monohaem. [Graphical view]
Gene3D	G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.
PANTHER	PTHR11961. Cyt_CIAB. 1 hit.
Pfam	PF00034. Cytochrom_C. 1 hit. [Graphical view]
PRINTS	PR00604. CYTCHRMECIAB.
ProDom	PD000375. Cyt_CIAB. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51007. CYTC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	791302.

Entry information

Entry name

CYC2_DROME

Accession

Primary (citable) accession number: P84029
Secondary accession number(s): P00033, P00034, Q9VJJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project