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Protein

Mothers against decapentaplegic homolog 3

Gene

SMAD3

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641ZincBy similarity
Metal bindingi109 – 1091ZincBy similarity
Metal bindingi121 – 1211ZincBy similarity
Metal bindingi126 – 1261ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-GGA-1502540. Signaling by Activin.
R-GGA-2173788. Downregulation of TGF-beta receptor signaling.
R-GGA-2173789. TGF-beta receptor signaling activates SMADs.
R-GGA-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 3
Short name:
MAD homolog 3
Short name:
Mad3
Short name:
Mothers against DPP homolog 3
Alternative name(s):
SMAD family member 3
Short name:
SMAD 3
Short name:
Smad3
Gene namesi
Name:SMAD3
Synonyms:MADH3
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 10

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Mothers against decapentaplegic homolog 3PRO_0000090860Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei424 – 4241PhosphoserinePROSITE-ProRule annotation
Modified residuei426 – 4261PhosphoserinePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP84023.

Expressioni

Gene expression databases

BgeeiENSGALG00000007870.

Interactioni

Subunit structurei

Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4.By similarity

Protein-protein interaction databases

BioGridi675429. 2 interactions.
STRINGi9031.ENSGALP00000012766.

Structurei

3D structure databases

ProteinModelPortaliP84023.
SMRiP84023. Positions 7-132, 221-417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 136127MH1PROSITE-ProRule annotationAdd
BLAST
Domaini233 – 426194MH2PROSITE-ProRule annotationAdd
BLAST

Domaini

The MH2 domain is sufficient to carry protein nuclear export.By similarity

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated
Contains 1 MH1 (MAD homology 1) domain.PROSITE-ProRule annotation
Contains 1 MH2 (MAD homology 2) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
GeneTreeiENSGT00760000119091.
HOGENOMiHOG000286018.
HOVERGENiHBG053353.
InParanoidiP84023.
KOiK04500.
OMAiAVELCEY.
OrthoDBiEOG091G082C.
PhylomeDBiP84023.
TreeFamiTF314923.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 1 hit.
InterProiIPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13703. PTHR13703. 1 hit.
PfamiPF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 1 hit.
PROSITEiPS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P84023-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSILPFTPP IVKRLLGWKK GEQNGQEEKW CEKAVKSLVK KLKKTGQLDE
60 70 80 90 100
LEKAITTQNI NTKCITIPRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH
110 120 130 140 150
HELRAMEMCE YAFNMKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEIPAEF
160 170 180 190 200
PPLDDYSHSI PENTNFPAGI EPQSNYIPET PPPGYLSEDG ETSDHQMNPS
210 220 230 240 250
MDAGSPNLSP NPMSPAHNNL DLQPVTYCEP AFWCSISYYE LNQRVGETFH
260 270 280 290 300
ASQPSMTVDG FTDPSNSERF CLGLLSNVNR NAAVELTRRH IGRGVRLYYI
310 320 330 340 350
GGEVFAECLS DSAIFVQSPN CNQRYGWHPA TVCKIPPGCN LKIFNNQEFA
360 370 380 390 400
ALLAQSVNQG FEAVYQLTRM CTIRMSFVKG WGAEYRRQTV TSTPCWIELH
410 420
LNGPLQWLDK VLTQMGSPSI RCSSVS
Length:426
Mass (Da):48,252
Last modified:July 5, 2004 - v1
Checksum:i58DC1F9A664EAC7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY391265 mRNA. Translation: AAQ89726.1.
RefSeqiNP_989806.1. NM_204475.1.
UniGeneiGga.28197.

Genome annotation databases

EnsembliENSGALT00000012780; ENSGALP00000012765; ENSGALG00000007870.
GeneIDi395132.
KEGGigga:395132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY391265 mRNA. Translation: AAQ89726.1.
RefSeqiNP_989806.1. NM_204475.1.
UniGeneiGga.28197.

3D structure databases

ProteinModelPortaliP84023.
SMRiP84023. Positions 7-132, 221-417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi675429. 2 interactions.
STRINGi9031.ENSGALP00000012766.

Proteomic databases

PaxDbiP84023.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000012780; ENSGALP00000012765; ENSGALG00000007870.
GeneIDi395132.
KEGGigga:395132.

Organism-specific databases

CTDi4088.

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
GeneTreeiENSGT00760000119091.
HOGENOMiHOG000286018.
HOVERGENiHBG053353.
InParanoidiP84023.
KOiK04500.
OMAiAVELCEY.
OrthoDBiEOG091G082C.
PhylomeDBiP84023.
TreeFamiTF314923.

Enzyme and pathway databases

ReactomeiR-GGA-1502540. Signaling by Activin.
R-GGA-2173788. Downregulation of TGF-beta receptor signaling.
R-GGA-2173789. TGF-beta receptor signaling activates SMADs.
R-GGA-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.

Miscellaneous databases

PROiP84023.

Gene expression databases

BgeeiENSGALG00000007870.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 1 hit.
InterProiIPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13703. PTHR13703. 1 hit.
PfamiPF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 1 hit.
PROSITEiPS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMAD3_CHICK
AccessioniPrimary (citable) accession number: P84023
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.