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Protein

Actin, indirect flight muscle

Gene

Act88F

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Required for proper formation of indirect flight muscle (IFM) myofibrils.
Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.

Miscellaneous

Abnormalities in Act88F-ifm37 flight muscles result from incorporation of the mutant actin isoform into assembling myofibrils.
In Drosophila there are 6 closely related actin genes.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: FlyBase

GO - Biological processi

  • cytoskeleton organization Source: FlyBase
  • muscle thin filament assembly Source: FlyBase
  • skeletal myofibril assembly Source: FlyBase

Keywordsi

Molecular functionMuscle protein
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiP83967

Names & Taxonomyi

Protein namesi
Recommended name:
Actin, indirect flight muscle
Alternative name(s):
Actin-88F
Gene namesi
Name:Act88F
ORF Names:CG5178
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0000047 Act88F

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Homozygous mutants are viable and fertile, but fail to form proper IFMs and are flightless. The IFMs of these mutants display mislocalized Smn and Actn.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi357 – 376Missing in allele Act88F-ifm(3)7. 1 PublicationAdd BLAST20

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000006681 – 2Removed in mature formBy similarity2
ChainiPRO_00000006693 – 376Actin, indirect flight muscleAdd BLAST374

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N-acetylaspartateBy similarity1
Modified residuei45Methionine sulfoxide1 Publication1
Modified residuei48Methionine sulfoxide1 Publication1

Post-translational modificationi

Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.1 Publication

Keywords - PTMi

Acetylation, Oxidation

Proteomic databases

PaxDbiP83967
PRIDEiP83967

Expressioni

Tissue specificityi

In adult thorax, expressed in the IFMs.1 Publication

Developmental stagei

Expressed throughout development. Expression is weak during embryonic stages and peaks during larval and pupal stages.1 Publication

Gene expression databases

BgeeiFBgn0000047
ExpressionAtlasiP83967 differential
GenevisibleiP83967 DM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
captQ9VPX64EBI-184828,EBI-15115807

Protein-protein interaction databases

BioGridi66947, 25 interactors
IntActiP83967, 13 interactors
STRINGi7227.FBpp0082597

Structurei

3D structure databases

ProteinModelPortaliP83967
SMRiP83967
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0676 Eukaryota
COG5277 LUCA
GeneTreeiENSGT00760000118957
InParanoidiP83967
KOiK05692
OMAiRIRNECT
OrthoDBiEOG091G08LD
PhylomeDBiP83967

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR020902 Actin/actin-like_CS
IPR004001 Actin_CS
PANTHERiPTHR11937 PTHR11937, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 1 hit
PRINTSiPR00190 ACTIN
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS00406 ACTINS_1, 1 hit
PS00432 ACTINS_2, 1 hit
PS01132 ACTINS_ACT_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCDDDAGALV IDNGSGMCKA GFAGDDAPRA VFPSIVGRPR HQGVMVGMGQ
60 70 80 90 100
KDSYVGDEAQ SKRGILTLKY PIEHGIITNW DDMEKIWHHT FYNELRVAPE
110 120 130 140 150
EHPVLLTEAP LNPKANREKM TQIMFETFNS PAMYVAIQAV LSLYASGRTT
160 170 180 190 200
GIVLDSGDGV SHTVPIYEGF ALPHAILRLD LAGRDLTDYL MKILTERGYS
210 220 230 240 250
FTTTAEREIV RDIKEKLCYV ALDFEQEMAT AAASTSLEKS YELPDGQVIT
260 270 280 290 300
IGNERFRCPE ALFQPSFLGM ESCGIHETVY NSIMKCDVDI RKDLYANSVL
310 320 330 340 350
SGGTTMYPGI ADRMQKEITA LAPSTIKIKI IAPPERKYSV WIGGSILASL
360 370
STFQQMWISK QEYDESGPGI VHRKCF
Length:376
Mass (Da):41,700
Last modified:June 7, 2004 - v1
Checksum:iC64C33A1674B7886
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200S → T in AAA28321 (PubMed:6405041).Curated1
Sequence conflicti213I → T in AAA28321 (PubMed:6405041).Curated1
Sequence conflicti226Q → D in AAA28321 (PubMed:6405041).Curated1
Sequence conflicti320A → T in AAA28321 (PubMed:6405041).Curated1
Sequence conflicti345S → L in AAA28321 (PubMed:6405041).Curated1
Sequence conflicti369G → S in AAA28321 (PubMed:6405041).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18826 Genomic DNA Translation: AAA28323.1
K02065 Genomic DNA Translation: AAA28322.1
AB003910 Genomic DNA Translation: BAA20058.1
AE014297 Genomic DNA Translation: AAF55198.1
M18830 Genomic DNA Translation: AAA28321.1
PIRiA03003 ATFF8
RefSeqiNP_524367.1, NM_079643.2
UniGeneiDm.5746

Genome annotation databases

EnsemblMetazoaiFBtr0083143; FBpp0082597; FBgn0000047
GeneIDi41885
KEGGidme:Dmel_CG5178

Similar proteinsi

Entry informationi

Entry nameiACT6_DROME
AccessioniPrimary (citable) accession number: P83967
Secondary accession number(s): P02575, P45893, Q9VF62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2004
Last modified: March 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health