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Protein

Root cyclotide 1

Gene
N/A
Organism
Viola hederacea (Australian violet)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably participates in a plant defense mechanism.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Root cyclotide 1
Alternative name(s):
Vhr1
OrganismiViola hederacea (Australian violet)
Taxonomic identifieri180952 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesViolaceaeViola

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3030Root cyclotide 1PRO_0000043608Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1 ↔ 30Cyclopeptide (Gly-Asn)
Disulfide bondi4 ↔ 21PROSITE-ProRule annotation1 Publication
Disulfide bondi8 ↔ 23PROSITE-ProRule annotation1 Publication
Disulfide bondi13 ↔ 28PROSITE-ProRule annotation1 Publication

Post-translational modificationi

This is a cyclic peptide.

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed in roots.1 Publication

Structurei

Secondary structure

1
30
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Beta strandi14 – 163Combined sources
Helixi17 – 193Combined sources
Beta strandi23 – 286Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VB8NMR-A4-30[»]
ProteinModelPortaliP83937.
SMRiP83937. Positions 4-29.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83937.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the cyclotide family. Bracelet subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012323. Cyclotide_bracelet_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
PIRSFiPIRSF037891. Cycloviolacin. 1 hit.
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60008. CYCLOTIDE_BRACELET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83937-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30
GIPCAESCVW IPCTVTALLG CSCSNKVCYN
Length:30
Mass (Da):3,136
Last modified:July 10, 2007 - v2
Checksum:iB6F6DAD5686B2937
GO

Mass spectrometryi

Molecular mass is 3115 Da from positions 1 - 30. Determined by ESI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VB8NMR-A4-30[»]
ProteinModelPortaliP83937.
SMRiP83937. Positions 4-29.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP83937.

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012323. Cyclotide_bracelet_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
PIRSFiPIRSF037891. Cycloviolacin. 1 hit.
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60008. CYCLOTIDE_BRACELET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Tissue-specific expression of head-to-tail cyclized miniproteins in Violaceae and structure determination of the root cyclotide Viola hederacea root cyclotide1."
    Trabi M., Craik D.J.
    Plant Cell 16:2204-2216(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, TISSUE SPECIFICITY, MASS SPECTROMETRY, STRUCTURE BY NMR, DISULFIDE BONDS.
    Tissue: Root.

Entry informationi

Entry nameiVHR1_VIOHE
AccessioniPrimary (citable) accession number: P83937
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 10, 2007
Last modified: December 9, 2015
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

This peptide is cyclic. The start position was chosen by similarity to OAK1 (kalata-B1) for which the DNA sequence is known.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.