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Protein

Chromobox protein homolog 1

Gene

Cbx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21Histone H3K9me21
Binding sitei23Histone H3A71
Binding sitei40Histone H3A71
Binding sitei42Histone H3A71
Binding sitei42Histone H3K9me21
Binding sitei45Histone H3K9me21
Binding sitei58Histone H3A71
Binding sitei60Histone H3A71
Binding sitei125PxVxL motif1
Binding sitei126PxVxL motif1
Binding sitei135PxVxL motif1
Binding sitei146PxVxL motif1
Binding sitei163PxVxL motif1
Binding sitei167PxVxL motif1
Binding sitei168PxVxL motif1
Binding sitei170PxVxL motif1

GO - Molecular functioni

  • enzyme binding Source: MGI
  • histone methyltransferase binding Source: MGI
  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: MGI

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 1
Alternative name(s):
Heterochromatin protein 1 homolog beta
Short name:
HP1 beta
Heterochromatin protein p25
M31
Modifier 1 protein
Gene namesi
Name:Cbx1
Synonyms:Cbx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:105369. Cbx1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23V → M: Abolishes interaction with SUV39H1. 1 Publication1
Mutagenesisi26V → R: Abolishes interaction with SUV39H1. 1 Publication1
Mutagenesisi161I → E: Abolishes homodimer formation. 1 Publication1
Mutagenesisi164Y → E: Abolishes homodimer formation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802001 – 185Chromobox protein homolog 1Add BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki9Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei89PhosphoserineBy similarity1
Modified residuei91PhosphoserineBy similarity1
Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki150Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei175PhosphoserineBy similarity1

Post-translational modificationi

Not phosphorylated.By similarity
Ubiquitinated.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP83917.
MaxQBiP83917.
PaxDbiP83917.
PeptideAtlasiP83917.
PRIDEiP83917.

2D gel databases

REPRODUCTION-2DPAGEiIPI00129466.

PTM databases

iPTMnetiP83917.
PhosphoSitePlusiP83917.

Expressioni

Tissue specificityi

In all adult and embryonic tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000018666.
CleanExiMM_CBX1.
ExpressionAtlasiP83917. baseline and differential.
GenevisibleiP83917. MM.

Interactioni

Subunit structurei

Homodimer. Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain. Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain. Interacts with SUV39H1, SETDB1, KMT5B and KMT5C. Interacts with PRDM6. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1. Interacts with INCENP. Interacts with SGO1; the CBX1 homodimer binds to one molecule of SGO1 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • enzyme binding Source: MGI
  • histone methyltransferase binding Source: MGI
  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi198534. 161 interactors.
DIPiDIP-30892N.
IntActiP83917. 156 interactors.
MINTiMINT-191134.
STRINGi10090.ENSMUSP00000091475.

Structurei

Secondary structure

1185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 13Combined sources3
Beta strandi22 – 31Combined sources10
Beta strandi33 – 47Combined sources15
Beta strandi51 – 54Combined sources4
Turni55 – 57Combined sources3
Helixi61 – 67Combined sources7
Turni68 – 72Combined sources5
Turni75 – 77Combined sources3
Beta strandi104 – 107Combined sources4
Helixi112 – 115Combined sources4
Beta strandi125 – 134Combined sources10
Beta strandi141 – 143Combined sources3
Beta strandi145 – 148Combined sources4
Helixi149 – 155Combined sources7
Helixi157 – 166Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AP0NMR-A10-80[»]
1DZ1NMR-A/B104-171[»]
1GUWNMR-A10-80[»]
1S4ZNMR-A/B104-176[»]
ProteinModelPortaliP83917.
SMRiP83917.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83917.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 79Chromo 1PROSITE-ProRule annotationAdd BLAST59
Domaini117 – 175Chromo 2; shadow subtypePROSITE-ProRule annotationAdd BLAST59

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410KDV3. Eukaryota.
ENOG4111H4A. LUCA.
GeneTreeiENSGT00510000046310.
HOGENOMiHOG000220852.
HOVERGENiHBG000400.
InParanoidiP83917.
KOiK11585.
OMAiQTWEPED.
OrthoDBiEOG091G0RBS.
PhylomeDBiP83917.
TreeFamiTF350503.

Family and domain databases

InterProiView protein in InterPro
IPR000953. Chromo/chromo_shadow_dom.
IPR017984. Chromo_dom_subgr.
IPR023780. Chromo_domain.
IPR008251. Chromo_shadow_dom.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
PfamiView protein in Pfam
PF00385. Chromo. 1 hit.
PF01393. Chromo_shadow. 1 hit.
PRINTSiPR00504. CHROMODOMAIN.
SMARTiView protein in SMART
SM00298. CHROMO. 2 hits.
SM00300. ChSh. 1 hit.
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiView protein in PROSITE
PS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 2 hits.

Sequencei

Sequence statusi: Complete.

P83917-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKQNKKKV EEVLEEEEEE YVVEKVLDRR VVKGKVEYLL KWKGFSDEDN
60 70 80 90 100
TWEPEENLDC PDLIAEFLQS QKTAHETDKS EGGKRKADSD SEDKGEESKP
110 120 130 140 150
KKKKEESEKP RGFARGLEPE RIIGATDSSG ELMFLMKWKN SDEADLVPAK
160 170 180
EANVKCPQVV ISFYEERLTW HSYPSEDDDK KDDKN
Length:185
Mass (Da):21,418
Last modified:June 7, 2004 - v1
Checksum:iBE687AF9C66E48E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56690 mRNA. Translation: CAA40018.1.
AK014215 mRNA. Translation: BAB29211.1.
CCDSiCCDS25303.1.
PIRiS26847.
RefSeqiNP_031648.1. NM_007622.3.
XP_006532162.1. XM_006532099.2.
XP_006532163.1. XM_006532100.2.
XP_011247006.1. XM_011248704.2.
XP_017169731.1. XM_017314242.1.
UniGeneiMm.29055.

Genome annotation databases

EnsembliENSMUST00000093943; ENSMUSP00000091475; ENSMUSG00000018666.
GeneIDi12412.
KEGGimmu:12412.
UCSCiuc007lck.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCBX1_MOUSE
AccessioniPrimary (citable) accession number: P83917
Secondary accession number(s): P23197
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: August 30, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references