Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chromobox protein homolog 1

Gene

CBX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21Histone H3K9me2By similarity1
Binding sitei23Histone H3A7By similarity1
Binding sitei40Histone H3A7By similarity1
Binding sitei42Histone H3A7By similarity1
Binding sitei42Histone H3K9me2By similarity1
Binding sitei45Histone H3K9me2By similarity1
Binding sitei58Histone H3A7By similarity1
Binding sitei60Histone H3A7By similarity1
Binding sitei125PxVxL motifBy similarity1
Binding sitei126PxVxL motifBy similarity1
Binding sitei135PxVxL motifBy similarity1
Binding sitei146PxVxL motifBy similarity1
Binding sitei163PxVxL motifBy similarity1
Binding sitei167PxVxL motifBy similarity1
Binding sitei168PxVxL motifBy similarity1
Binding sitei170PxVxL motifBy similarity1

GO - Molecular functioni

  • chromatin binding Source: ProtInc
  • enzyme binding Source: UniProtKB
  • histone methyltransferase binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108468-MONOMER.
SIGNORiP83916.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 1
Alternative name(s):
HP1Hsbeta
Heterochromatin protein 1 homolog beta
Short name:
HP1 beta
Heterochromatin protein p25
M31
Modifier 1 protein
p25beta
Gene namesi
Name:CBX1
Synonyms:CBX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:1551. CBX1.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • chromocenter Source: Ensembl
  • chromosome, centromeric region Source: UniProtKB
  • female pronucleus Source: Ensembl
  • male pronucleus Source: Ensembl
  • nuclear chromosome, telomeric region Source: BHF-UCL
  • nuclear heterochromatin Source: ProtInc
  • nucleoplasm Source: ProtInc
  • pericentric heterochromatin Source: Ensembl
  • spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi161I → E: Abolishes homodimer formation and binding to EMSY. 1 Publication1

Organism-specific databases

DisGeNETi10951.
OpenTargetsiENSG00000108468.
PharmGKBiPA26126.

Chemistry databases

ChEMBLiCHEMBL1741193.

Polymorphism and mutation databases

BioMutaiCBX1.
DMDMi48428808.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000801991 – 185Chromobox protein homolog 1Add BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki9Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei89PhosphoserineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei175PhosphoserineCombined sources1

Post-translational modificationi

Not phosphorylated.
Ubiquitinated.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP83916.
PaxDbiP83916.
PeptideAtlasiP83916.
PRIDEiP83916.
TopDownProteomicsiP83916.

PTM databases

iPTMnetiP83916.
PhosphoSitePlusiP83916.
SwissPalmiP83916.

Expressioni

Tissue specificityi

Expressed in all adult and embryonic tissues.

Gene expression databases

BgeeiENSG00000108468.
CleanExiHS_CBX1.
ExpressionAtlasiP83916. baseline and differential.
GenevisibleiP83916. HS.

Organism-specific databases

HPAiCAB012265.

Interactioni

Subunit structurei

Homodimer. Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain. Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain. Interacts with SUV39H1 and SETDB1, KMT5B and KMT5C. Interacts with PRDM6. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1. Interacts with INCENP. Interacts with SGO1; the CBX1 homodimer binds to one molecule of SGO1.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADNPQ9H2P03EBI-78129,EBI-1764854
ADNP2Q6IQ323EBI-78129,EBI-2838654
CHAF1AQ131113EBI-78129,EBI-1020839
HIST1H3DP684319EBI-78129,EBI-79722
NR2F6P105882EBI-78129,EBI-2681496
SUV39H1O434633EBI-78129,EBI-349968
ZNF280CQ8ND823EBI-78129,EBI-8831272

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • histone methyltransferase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi116151. 71 interactors.
DIPiDIP-28135N.
IntActiP83916. 105 interactors.
MINTiMINT-1378005.
STRINGi9606.ENSP00000225603.

Chemistry databases

BindingDBiP83916.

Structurei

Secondary structure

1185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 32Combined sources10
Beta strandi35 – 42Combined sources8
Helixi47 – 49Combined sources3
Beta strandi51 – 54Combined sources4
Helixi55 – 57Combined sources3
Helixi61 – 68Combined sources8
Helixi112 – 115Combined sources4
Beta strandi119 – 128Combined sources10
Beta strandi131 – 138Combined sources8
Beta strandi145 – 148Combined sources4
Helixi149 – 155Combined sources7
Helixi157 – 165Combined sources9
Beta strandi168 – 172Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FMMX-ray1.80A/B/C/D104-175[»]
3F2UX-ray1.80A20-73[»]
3Q6SX-ray1.93A/B/C/D108-185[»]
5T1GX-ray1.90A108-185[»]
ProteinModelPortaliP83916.
SMRiP83916.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83916.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 79Chromo 1PROSITE-ProRule annotationAdd BLAST59
Domaini117 – 175Chromo 2; shadow subtypePROSITE-ProRule annotationAdd BLAST59

Sequence similaritiesi

Contains 2 chromo domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410KDV3. Eukaryota.
ENOG4111H4A. LUCA.
GeneTreeiENSGT00510000046310.
HOGENOMiHOG000220852.
HOVERGENiHBG000400.
InParanoidiP83916.
KOiK11585.
OMAiWKGTDDA.
OrthoDBiEOG091G0RBS.
PhylomeDBiP83916.
TreeFamiTF350503.

Family and domain databases

InterProiIPR000953. Chromo/shadow_dom.
IPR017984. Chromo_dom_subgr.
IPR023780. Chromo_domain.
IPR008251. Chromo_shadow_dom.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
PF01393. Chromo_shadow. 1 hit.
[Graphical view]
PRINTSiPR00504. CHROMODOMAIN.
SMARTiSM00298. CHROMO. 2 hits.
SM00300. ChSh. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKQNKKKV EEVLEEEEEE YVVEKVLDRR VVKGKVEYLL KWKGFSDEDN
60 70 80 90 100
TWEPEENLDC PDLIAEFLQS QKTAHETDKS EGGKRKADSD SEDKGEESKP
110 120 130 140 150
KKKKEESEKP RGFARGLEPE RIIGATDSSG ELMFLMKWKN SDEADLVPAK
160 170 180
EANVKCPQVV ISFYEERLTW HSYPSEDDDK KDDKN
Length:185
Mass (Da):21,418
Last modified:June 7, 2004 - v1
Checksum:iBE687AF9C66E48E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35451 mRNA. Translation: AAB81548.1.
BC002609 mRNA. Translation: AAH02609.1.
BC021302 mRNA. Translation: AAH21302.1.
CCDSiCCDS11525.1.
PIRiG02080.
RefSeqiNP_001120700.1. NM_001127228.1.
NP_006798.1. NM_006807.4.
UniGeneiHs.77254.

Genome annotation databases

EnsembliENST00000225603; ENSP00000225603; ENSG00000108468.
ENST00000393408; ENSP00000377060; ENSG00000108468.
GeneIDi10951.
KEGGihsa:10951.

Cross-referencesi

Web resourcesi

Wikipedia

Heterochromatin protein 1 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35451 mRNA. Translation: AAB81548.1.
BC002609 mRNA. Translation: AAH02609.1.
BC021302 mRNA. Translation: AAH21302.1.
CCDSiCCDS11525.1.
PIRiG02080.
RefSeqiNP_001120700.1. NM_001127228.1.
NP_006798.1. NM_006807.4.
UniGeneiHs.77254.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FMMX-ray1.80A/B/C/D104-175[»]
3F2UX-ray1.80A20-73[»]
3Q6SX-ray1.93A/B/C/D108-185[»]
5T1GX-ray1.90A108-185[»]
ProteinModelPortaliP83916.
SMRiP83916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116151. 71 interactors.
DIPiDIP-28135N.
IntActiP83916. 105 interactors.
MINTiMINT-1378005.
STRINGi9606.ENSP00000225603.

Chemistry databases

BindingDBiP83916.
ChEMBLiCHEMBL1741193.

PTM databases

iPTMnetiP83916.
PhosphoSitePlusiP83916.
SwissPalmiP83916.

Polymorphism and mutation databases

BioMutaiCBX1.
DMDMi48428808.

Proteomic databases

EPDiP83916.
PaxDbiP83916.
PeptideAtlasiP83916.
PRIDEiP83916.
TopDownProteomicsiP83916.

Protocols and materials databases

DNASUi10951.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225603; ENSP00000225603; ENSG00000108468.
ENST00000393408; ENSP00000377060; ENSG00000108468.
GeneIDi10951.
KEGGihsa:10951.

Organism-specific databases

CTDi10951.
DisGeNETi10951.
GeneCardsiCBX1.
HGNCiHGNC:1551. CBX1.
HPAiCAB012265.
MIMi604511. gene.
neXtProtiNX_P83916.
OpenTargetsiENSG00000108468.
PharmGKBiPA26126.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410KDV3. Eukaryota.
ENOG4111H4A. LUCA.
GeneTreeiENSGT00510000046310.
HOGENOMiHOG000220852.
HOVERGENiHBG000400.
InParanoidiP83916.
KOiK11585.
OMAiWKGTDDA.
OrthoDBiEOG091G0RBS.
PhylomeDBiP83916.
TreeFamiTF350503.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108468-MONOMER.
SIGNORiP83916.

Miscellaneous databases

EvolutionaryTraceiP83916.
GeneWikiiCBX1.
GenomeRNAii10951.
PROiP83916.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108468.
CleanExiHS_CBX1.
ExpressionAtlasiP83916. baseline and differential.
GenevisibleiP83916. HS.

Family and domain databases

InterProiIPR000953. Chromo/shadow_dom.
IPR017984. Chromo_dom_subgr.
IPR023780. Chromo_domain.
IPR008251. Chromo_shadow_dom.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
PF01393. Chromo_shadow. 1 hit.
[Graphical view]
PRINTSiPR00504. CHROMODOMAIN.
SMARTiSM00298. CHROMO. 2 hits.
SM00300. ChSh. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBX1_HUMAN
AccessioniPrimary (citable) accession number: P83916
Secondary accession number(s): P23197
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.