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Protein

Chromobox protein homolog 1

Gene

CBX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211Histone H3K9me2By similarity
Binding sitei23 – 231Histone H3A7By similarity
Binding sitei40 – 401Histone H3A7By similarity
Binding sitei42 – 421Histone H3A7By similarity
Binding sitei42 – 421Histone H3K9me2By similarity
Binding sitei45 – 451Histone H3K9me2By similarity
Binding sitei58 – 581Histone H3A7By similarity
Binding sitei60 – 601Histone H3A7By similarity
Binding sitei125 – 1251PxVxL motifBy similarity
Binding sitei126 – 1261PxVxL motifBy similarity
Binding sitei135 – 1351PxVxL motifBy similarity
Binding sitei146 – 1461PxVxL motifBy similarity
Binding sitei163 – 1631PxVxL motifBy similarity
Binding sitei167 – 1671PxVxL motifBy similarity
Binding sitei168 – 1681PxVxL motifBy similarity
Binding sitei170 – 1701PxVxL motifBy similarity

GO - Molecular functioni

  • chromatin binding Source: ProtInc
  • enzyme binding Source: UniProtKB
  • histone methyltransferase binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

SIGNORiP83916.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 1
Alternative name(s):
HP1Hsbeta
Heterochromatin protein 1 homolog beta
Short name:
HP1 beta
Heterochromatin protein p25
M31
Modifier 1 protein
p25beta
Gene namesi
Name:CBX1
Synonyms:CBX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:1551. CBX1.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • chromocenter Source: Ensembl
  • chromosome, centromeric region Source: UniProtKB
  • female pronucleus Source: Ensembl
  • male pronucleus Source: Ensembl
  • nuclear chromosome, telomeric region Source: BHF-UCL
  • nuclear heterochromatin Source: ProtInc
  • nucleoplasm Source: ProtInc
  • pericentric heterochromatin Source: Ensembl
  • spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi161 – 1611I → E: Abolishes homodimer formation and binding to EMSY. 1 Publication

Organism-specific databases

PharmGKBiPA26126.

Chemistry

ChEMBLiCHEMBL1741193.

Polymorphism and mutation databases

BioMutaiCBX1.
DMDMi48428808.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Chromobox protein homolog 1PRO_0000080199Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki9 – 9Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei89 – 891PhosphoserineCombined sources
Modified residuei91 – 911PhosphoserineCombined sources
Modified residuei175 – 1751PhosphoserineCombined sources

Post-translational modificationi

Not phosphorylated.
Ubiquitinated.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP83916.
PaxDbiP83916.
PeptideAtlasiP83916.
PRIDEiP83916.
TopDownProteomicsiP83916.

PTM databases

iPTMnetiP83916.
PhosphoSiteiP83916.
SwissPalmiP83916.

Expressioni

Tissue specificityi

Expressed in all adult and embryonic tissues.

Gene expression databases

BgeeiENSG00000108468.
CleanExiHS_CBX1.
ExpressionAtlasiP83916. baseline and differential.
GenevisibleiP83916. HS.

Organism-specific databases

HPAiCAB012265.

Interactioni

Subunit structurei

Homodimer. Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain. Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain. Interacts with SUV39H1 and SETDB1, KMT5B and KMT5C. Interacts with PRDM6. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADNPQ9H2P02EBI-78129,EBI-1764854
ADNP2Q6IQ322EBI-78129,EBI-2838654
HIST1H3DP684319EBI-78129,EBI-79722
NR2F6P105882EBI-78129,EBI-2681496

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • histone methyltransferase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi116151. 71 interactions.
DIPiDIP-28135N.
IntActiP83916. 58 interactions.
MINTiMINT-1378005.
STRINGi9606.ENSP00000225603.

Chemistry

BindingDBiP83916.

Structurei

Secondary structure

1
185
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 3210Combined sources
Beta strandi35 – 428Combined sources
Helixi47 – 493Combined sources
Beta strandi51 – 544Combined sources
Helixi55 – 573Combined sources
Helixi61 – 688Combined sources
Helixi112 – 1154Combined sources
Beta strandi119 – 12810Combined sources
Beta strandi131 – 1388Combined sources
Beta strandi145 – 1484Combined sources
Helixi149 – 1557Combined sources
Helixi157 – 1659Combined sources
Beta strandi168 – 1725Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FMMX-ray1.80A/B/C/D104-175[»]
3F2UX-ray1.80A20-73[»]
3Q6SX-ray1.93A/B/C/D108-185[»]
ProteinModelPortaliP83916.
SMRiP83916. Positions 27-80, 110-175.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83916.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 7959Chromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini117 – 17559Chromo 2; shadow subtypePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 chromo domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410KDV3. Eukaryota.
ENOG4111H4A. LUCA.
GeneTreeiENSGT00510000046310.
HOGENOMiHOG000220852.
HOVERGENiHBG000400.
InParanoidiP83916.
KOiK11585.
OMAiWKGTDDA.
OrthoDBiEOG091G0RBS.
PhylomeDBiP83916.
TreeFamiTF350503.

Family and domain databases

InterProiIPR000953. Chromo/shadow_dom.
IPR017984. Chromo_dom_subgr.
IPR023780. Chromo_domain.
IPR008251. Chromo_shadow_dom.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
PF01393. Chromo_shadow. 1 hit.
[Graphical view]
PRINTSiPR00504. CHROMODOMAIN.
SMARTiSM00298. CHROMO. 2 hits.
SM00300. ChSh. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKQNKKKV EEVLEEEEEE YVVEKVLDRR VVKGKVEYLL KWKGFSDEDN
60 70 80 90 100
TWEPEENLDC PDLIAEFLQS QKTAHETDKS EGGKRKADSD SEDKGEESKP
110 120 130 140 150
KKKKEESEKP RGFARGLEPE RIIGATDSSG ELMFLMKWKN SDEADLVPAK
160 170 180
EANVKCPQVV ISFYEERLTW HSYPSEDDDK KDDKN
Length:185
Mass (Da):21,418
Last modified:June 7, 2004 - v1
Checksum:iBE687AF9C66E48E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35451 mRNA. Translation: AAB81548.1.
BC002609 mRNA. Translation: AAH02609.1.
BC021302 mRNA. Translation: AAH21302.1.
CCDSiCCDS11525.1.
PIRiG02080.
RefSeqiNP_001120700.1. NM_001127228.1.
NP_006798.1. NM_006807.4.
UniGeneiHs.77254.

Genome annotation databases

EnsembliENST00000225603; ENSP00000225603; ENSG00000108468.
ENST00000393408; ENSP00000377060; ENSG00000108468.
GeneIDi10951.
KEGGihsa:10951.

Cross-referencesi

Web resourcesi

Wikipedia

Heterochromatin protein 1 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35451 mRNA. Translation: AAB81548.1.
BC002609 mRNA. Translation: AAH02609.1.
BC021302 mRNA. Translation: AAH21302.1.
CCDSiCCDS11525.1.
PIRiG02080.
RefSeqiNP_001120700.1. NM_001127228.1.
NP_006798.1. NM_006807.4.
UniGeneiHs.77254.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FMMX-ray1.80A/B/C/D104-175[»]
3F2UX-ray1.80A20-73[»]
3Q6SX-ray1.93A/B/C/D108-185[»]
ProteinModelPortaliP83916.
SMRiP83916. Positions 27-80, 110-175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116151. 71 interactions.
DIPiDIP-28135N.
IntActiP83916. 58 interactions.
MINTiMINT-1378005.
STRINGi9606.ENSP00000225603.

Chemistry

BindingDBiP83916.
ChEMBLiCHEMBL1741193.

PTM databases

iPTMnetiP83916.
PhosphoSiteiP83916.
SwissPalmiP83916.

Polymorphism and mutation databases

BioMutaiCBX1.
DMDMi48428808.

Proteomic databases

EPDiP83916.
PaxDbiP83916.
PeptideAtlasiP83916.
PRIDEiP83916.
TopDownProteomicsiP83916.

Protocols and materials databases

DNASUi10951.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225603; ENSP00000225603; ENSG00000108468.
ENST00000393408; ENSP00000377060; ENSG00000108468.
GeneIDi10951.
KEGGihsa:10951.

Organism-specific databases

CTDi10951.
GeneCardsiCBX1.
HGNCiHGNC:1551. CBX1.
HPAiCAB012265.
MIMi604511. gene.
neXtProtiNX_P83916.
PharmGKBiPA26126.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410KDV3. Eukaryota.
ENOG4111H4A. LUCA.
GeneTreeiENSGT00510000046310.
HOGENOMiHOG000220852.
HOVERGENiHBG000400.
InParanoidiP83916.
KOiK11585.
OMAiWKGTDDA.
OrthoDBiEOG091G0RBS.
PhylomeDBiP83916.
TreeFamiTF350503.

Enzyme and pathway databases

SIGNORiP83916.

Miscellaneous databases

EvolutionaryTraceiP83916.
GeneWikiiCBX1.
GenomeRNAii10951.
PROiP83916.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108468.
CleanExiHS_CBX1.
ExpressionAtlasiP83916. baseline and differential.
GenevisibleiP83916. HS.

Family and domain databases

InterProiIPR000953. Chromo/shadow_dom.
IPR017984. Chromo_dom_subgr.
IPR023780. Chromo_domain.
IPR008251. Chromo_shadow_dom.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
PF01393. Chromo_shadow. 1 hit.
[Graphical view]
PRINTSiPR00504. CHROMODOMAIN.
SMARTiSM00298. CHROMO. 2 hits.
SM00300. ChSh. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBX1_HUMAN
AccessioniPrimary (citable) accession number: P83916
Secondary accession number(s): P23197
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 7, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.