P83900 (RPGF5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rap guanine nucleotide exchange factor 5

Alternative name(s):
M-Ras-regulated Rap GEF
Short name=MR-GEF
Short name=rMR-GEF

Gene names

Name:	Rapgef5
Synonyms:	Mrgef

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation By similarity. UniProtKB Q92565
Subcellular location	Nucleus By similarity UniProtKB Q92565.
Tissue specificity	In the embryo, expressed in young neurons of the developing telencephalon, diencephalon and hindbrain. Not expressed in progenitor cells in the ventricular zone. Ref.1
Developmental stage	Expression is first detected at embryonic day 13. Ref.1
Induction	Down-regulated in nerve growth factor-treated PC12 cells. Ref.1
Sequence similarities	Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain.

Ontologies

Keywords
Cellular component	Nucleus
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Rap guanine nucleotide exchange factor 5		PRO_0000068875
Regions
	Domain	67 – 200	134	N-terminal Ras-GEF
	Domain	344 – 579	236	Ras-GEF

Sequences

	Sequence		Length	Mass (Da)
	P83900 [UniParc]. Last modified May 24, 2004. Version 1. Checksum: 9B3A0B49944E40FC	FASTA	580	67,601
	10 20 30 40 50 60 MGSSRLRVFD PPLERKDSAA LSERQLPLPT FDVPYFKYID EEDEDDEWGS RSQSSTEDDS 70 80 90 100 110 120 VDSLLSDRYV VVSGTPEKIL EHLLNDLHLA EVQHKETETL LDDFLLTYTV FMTTDDLCQA 130 140 150 160 170 180 LLRHYSAKKY QGEEENSDVP CRKRKVLHLV SQWISLYKDW LHEDEHSKMF LKTIYRNVLD 190 200 210 220 230 240 DVYEYPILEK ELKEFQKILG MHRRHTVDEY SPQKKNKALF HQFSLKENWL QHRGTVAETE 250 260 270 280 290 300 EIFCHVYVTE HSYISVKAKV SSTAQEILKV VAEKLQRAEE DLALVAIMFS GEKHEFQPND 310 320 330 340 350 360 LAISKSLEAS GRIYVYRKDL ADTLNPFAEN EESQQRSMRI LGMNTWDLAL ELMSFDWSLF 370 380 390 400 410 420 NSIHEQELIY FTFSRQGSGE HTVNLSLLLQ RCNEVQLWVA TEILLCSQLG KRVQLVKKFI 430 440 450 460 470 480 KIAAHCKAQQ NLNSFFAIVM GLNTASVSRL SQTWEKIPGK FKKLFSELES LTDPSLNHKA 490 500 510 520 530 540 YRDAFKKMKP PKIPFMPLLL KDVTFIHEGN KTFLDNLVNF EKLHMIADTV RTLRHCRTNQ 550 560 570 580 FGSDMSPKEQ QELKSYVNHL YVIDSQQALF ELSHRLEPRA

References

[1]

"Expression of the guanine nucleotide exchange factor, mr-gef, is regulated during the differentiation of specific subsets of telencephalic neurons." Bithell A., Alberta J., Hornby F., Stiles C.D., Williams B.P. Brain Res. Dev. Brain Res. 146:107-118(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: Sprague-Dawley. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY390379 mRNA. Translation: AAR83745.1.
IPI	IPI00417384.
RefSeq	NP_001041380.1. NM_001047915.1.
UniGene	Rn.22381.
3D structure databases
ProteinModelPortal	P83900.
SMR	P83900. Positions 240-330.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000007112.
Proteomic databases
PaxDb	P83900.
PRIDE	P83900.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	362799.
KEGG	rno:362799.
UCSC	RGD:1561668. rat.
Organism-specific databases
CTD	9771.
RGD	1561668. Rapgef5.
Phylogenomic databases
eggNOG	NOG236726.
HOVERGEN	HBG056985.
KO	K08019.
Gene expression databases
Genevestigator	P83900.
Family and domain databases
Gene3D	1.10.840.10. 1 hit.
InterPro	IPR000651. Ras-like_Gua-exchang_fac_N. IPR019804. Ras_G-nucl-exch_fac_CS. IPR008937. Ras_GEF. IPR023578. Ras_GEF_dom. IPR001895. RasGRF_CDC25. [Graphical view]
PANTHER	PTHR23113. PTHR23113. 1 hit.
Pfam	PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. [Graphical view]
SMART	SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. [Graphical view]
SUPFAM	SSF48366. Ras_GEF. 1 hit.
PROSITE	PS00720. RASGEF. 1 hit. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	681302.

Entry information

Entry name

RPGF5_RAT

Accession

Primary (citable) accession number: P83900

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 24, 2004
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program