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Protein

Rap guanine nucleotide exchange factor 5

Gene

Rapgef5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity).By similarity

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: UniProtKB-KW

GO - Biological processi

  1. regulation of small GTPase mediated signal transduction Source: InterPro
  2. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Rap guanine nucleotide exchange factor 5
Alternative name(s):
M-Ras-regulated Rap GEF
Short name:
MR-GEF
Short name:
rMR-GEF
Gene namesi
Name:Rapgef5
Synonyms:Mrgef
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1561668. Rapgef5.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Rap guanine nucleotide exchange factor 5PRO_0000068875Add
BLAST

Proteomic databases

PaxDbiP83900.
PRIDEiP83900.

Expressioni

Tissue specificityi

In the embryo, expressed in young neurons of the developing telencephalon, diencephalon and hindbrain. Not expressed in progenitor cells in the ventricular zone.1 Publication

Developmental stagei

Expression is first detected at embryonic day 13.1 Publication

Inductioni

Down-regulated in nerve growth factor-treated PC12 cells.1 Publication

Gene expression databases

GenevestigatoriP83900.

Interactioni

Protein-protein interaction databases

BioGridi263730. 1 interaction.
STRINGi10116.ENSRNOP00000007112.

Structurei

3D structure databases

ProteinModelPortaliP83900.
SMRiP83900. Positions 240-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 200134N-terminal Ras-GEFPROSITE-ProRule annotationCuratedAdd
BLAST
Domaini344 – 579236Ras-GEFPROSITE-ProRule annotationCuratedAdd
BLAST

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotationCurated
Contains 1 Ras-GEF domain.PROSITE-ProRule annotationCurated

Phylogenomic databases

eggNOGiNOG236726.
HOVERGENiHBG056985.
InParanoidiP83900.
KOiK08019.
PhylomeDBiP83900.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSRLRVFD PPLERKDSAA LSERQLPLPT FDVPYFKYID EEDEDDEWGS
60 70 80 90 100
RSQSSTEDDS VDSLLSDRYV VVSGTPEKIL EHLLNDLHLA EVQHKETETL
110 120 130 140 150
LDDFLLTYTV FMTTDDLCQA LLRHYSAKKY QGEEENSDVP CRKRKVLHLV
160 170 180 190 200
SQWISLYKDW LHEDEHSKMF LKTIYRNVLD DVYEYPILEK ELKEFQKILG
210 220 230 240 250
MHRRHTVDEY SPQKKNKALF HQFSLKENWL QHRGTVAETE EIFCHVYVTE
260 270 280 290 300
HSYISVKAKV SSTAQEILKV VAEKLQRAEE DLALVAIMFS GEKHEFQPND
310 320 330 340 350
LAISKSLEAS GRIYVYRKDL ADTLNPFAEN EESQQRSMRI LGMNTWDLAL
360 370 380 390 400
ELMSFDWSLF NSIHEQELIY FTFSRQGSGE HTVNLSLLLQ RCNEVQLWVA
410 420 430 440 450
TEILLCSQLG KRVQLVKKFI KIAAHCKAQQ NLNSFFAIVM GLNTASVSRL
460 470 480 490 500
SQTWEKIPGK FKKLFSELES LTDPSLNHKA YRDAFKKMKP PKIPFMPLLL
510 520 530 540 550
KDVTFIHEGN KTFLDNLVNF EKLHMIADTV RTLRHCRTNQ FGSDMSPKEQ
560 570 580
QELKSYVNHL YVIDSQQALF ELSHRLEPRA
Length:580
Mass (Da):67,601
Last modified:May 24, 2004 - v1
Checksum:i9B3A0B49944E40FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390379 mRNA. Translation: AAR83745.1.
RefSeqiNP_001041380.1. NM_001047915.1.
UniGeneiRn.22381.

Genome annotation databases

GeneIDi362799.
KEGGirno:362799.
UCSCiRGD:1561668. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390379 mRNA. Translation: AAR83745.1.
RefSeqiNP_001041380.1. NM_001047915.1.
UniGeneiRn.22381.

3D structure databases

ProteinModelPortaliP83900.
SMRiP83900. Positions 240-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263730. 1 interaction.
STRINGi10116.ENSRNOP00000007112.

Proteomic databases

PaxDbiP83900.
PRIDEiP83900.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi362799.
KEGGirno:362799.
UCSCiRGD:1561668. rat.

Organism-specific databases

CTDi9771.
RGDi1561668. Rapgef5.

Phylogenomic databases

eggNOGiNOG236726.
HOVERGENiHBG056985.
InParanoidiP83900.
KOiK08019.
PhylomeDBiP83900.

Miscellaneous databases

NextBioi681302.
PROiP83900.

Gene expression databases

GenevestigatoriP83900.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of the guanine nucleotide exchange factor, mr-gef, is regulated during the differentiation of specific subsets of telencephalic neurons."
    Bithell A., Alberta J., Hornby F., Stiles C.D., Williams B.P.
    Brain Res. Dev. Brain Res. 146:107-118(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: Sprague-DawleyImported.
    Tissue: Brain cortexImported.

Entry informationi

Entry nameiRPGF5_RAT
AccessioniPrimary (citable) accession number: P83900
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: January 7, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.