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Protein

PHD finger-like domain-containing protein 5A

Gene

Phf5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha. Also involved in pre-mRNA splicing (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processmRNA processing, mRNA splicing, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
PHD finger-like domain-containing protein 5A
Short name:
PHD finger-like domain protein 5A
Alternative name(s):
Splicing factor 3B-associated 14 kDa protein
Short name:
SF3b14b
Gene namesi
Name:Phf5a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2156864. Phf5a.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: GO_Central
  • nuclear matrix Source: MGI
  • nuclear speck Source: UniProtKB
  • precatalytic spliceosome Source: GO_Central
  • U12-type spliceosomal complex Source: MGI
  • U2 snRNP Source: UniProtKB

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002187172 – 110PHD finger-like domain-containing protein 5AAdd BLAST109

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei3N6-acetyllysineCombined sources1
Modified residuei94PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP83870.
MaxQBiP83870.
PaxDbiP83870.
PeptideAtlasiP83870.
PRIDEiP83870.

PTM databases

iPTMnetiP83870.
PhosphoSitePlusiP83870.

Expressioni

Tissue specificityi

Expressed in primary spermatocytes (at protein level). Ubiquitously expressed in pre- and postnatal tissues.2 Publications

Gene expression databases

BgeeiENSMUSG00000061360.
CleanExiMM_PHF5A.
GenevisibleiP83870. MM.

Interactioni

Subunit structurei

Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A/SF3B14B, and DDX42/SF3B125. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Component of the U11/U12 snRNPs that are part of the U12-type spliceosome (By similarity). Interacts (via N-terminus) with U2AF1 and SRSF5; acts to bridge the two. Interacts (via C-terminus) with EP400 and DDX1; acts to bridge the two.By similarity1 Publication

Protein-protein interaction databases

BioGridi212875. 1 interactor.
IntActiP83870. 2 interactors.
MINTiMINT-4107454.
STRINGi10090.ENSMUSP00000023117.

Structurei

3D structure databases

ProteinModelPortaliP83870.
SMRiP83870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PHF5 family.Curated

Phylogenomic databases

eggNOGiKOG1705. Eukaryota.
ENOG4111JIM. LUCA.
GeneTreeiENSGT00390000018518.
HOGENOMiHOG000168285.
HOVERGENiHBG053587.
InParanoidiP83870.
KOiK12834.
OMAiDAYYCRE.
OrthoDBiEOG091G0W8V.
PhylomeDBiP83870.
TreeFamiTF105627.

Family and domain databases

InterProiView protein in InterPro
IPR005345. PHF5.
PANTHERiPTHR13120. PTHR13120. 1 hit.
PfamiView protein in Pfam
PF03660. PHF5. 1 hit.
PIRSFiPIRSF016468. PHF5. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKHHPDLIF CRKQAGVAIG RLCEKCDGKC VICDSYVRPC TLVRICDECN
60 70 80 90 100
YGSYQGRCVI CGGPGVSDAY YCKECTIQEK DRDGCPKIVN LGSSKTDLFY
110
ERKKYGFKKR
Length:110
Mass (Da):12,405
Last modified:April 26, 2004 - v1
Checksum:i90F7469DE7292BF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF479286 Genomic DNA. Translation: AAM49735.1.
AF479288 mRNA. Translation: AAM49736.1.
AK003520 mRNA. Translation: BAB22833.1.
AK143590 mRNA. Translation: BAE25453.1.
AK163247 mRNA. Translation: BAE37255.1.
AK167932 mRNA. Translation: BAE39936.1.
AK167955 mRNA. Translation: BAE39955.1.
BC025161 mRNA. Translation: AAH25161.1.
CCDSiCCDS27674.1.
PIRiJC7851.
RefSeqiNP_081013.1. NM_026737.3.
UniGeneiMm.271715.

Genome annotation databases

EnsembliENSMUST00000023117; ENSMUSP00000023117; ENSMUSG00000061360.
GeneIDi68479.
KEGGimmu:68479.
UCSCiuc007wxo.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPHF5A_MOUSE
AccessioniPrimary (citable) accession number: P83870
Secondary accession number(s): Q3TQX7, Q9UH06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: June 7, 2017
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families