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Protein

Prostaglandin E synthase 3

Gene

Ptges3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) (PubMed:10922363). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway (By similarity).By similarity1 Publication

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate.1 Publication

Pathwayi: prostaglandin biosynthesis

This protein is involved in the pathway prostaglandin biosynthesis, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway prostaglandin biosynthesis and in Lipid metabolism.

GO - Molecular functioni

  • enzyme binding Source: RGD
  • Hsp90 protein binding Source: RGD
  • p53 binding Source: RGD
  • prostaglandin-E synthase activity Source: UniProtKB
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

  • negative regulation of cell death Source: RGD
  • positive regulation of gene expression Source: RGD
  • prostaglandin biosynthetic process Source: UniProtKB
  • prostaglandin metabolic process Source: RGD
  • sensory perception of pain Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

Enzyme and pathway databases

ReactomeiR-RNO-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-RNO-3371511. HSF1 activation.
R-RNO-3371568. Attenuation phase.
SABIO-RKP83868.
UniPathwayiUPA00662.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E synthase 3 (EC:5.3.99.31 Publication)
Alternative name(s):
Cytosolic prostaglandin E2 synthase
Short name:
cPGES
Hsp90 co-chaperone
Progesterone receptor complex p23
Telomerase-binding protein p23
Gene namesi
Name:Ptges3By similarity
Synonyms:TebpBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1561913. Ptges3.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • actin filament Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • neuronal cell body Source: RGD
  • neuron projection Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160Prostaglandin E synthase 3PRO_0000291382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331N6-acetyllysineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei85 – 851PhosphoserineBy similarity
Modified residuei100 – 1001PhosphoserineBy similarity
Modified residuei113 – 1131PhosphoserineCombined sources
Modified residuei118 – 1181PhosphoserineBy similarity
Modified residuei148 – 1481PhosphoserineCombined sources
Modified residuei151 – 1511PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP83868.
PRIDEiP83868.

PTM databases

iPTMnetiP83868.
PhosphoSiteiP83868.

Expressioni

Tissue specificityi

Widely expressed with highest levels in testis.1 Publication

Inductioni

In brain, by LPS.1 Publication

Gene expression databases

ExpressionAtlasiP83868. baseline.
GenevisibleiP83868. RN.

Interactioni

Subunit structurei

Binds to the progesterone receptor. Interacts with TERT; the interaction, together with HSP90AA1, is required for correct assembly and stabilization of the telomerase holoenzyme complex. Interacts (via PXLE motif) with EGLN1/PHD2, recruiting EGLN1/PHD2 to the HSP90 pathway to facilitate HIF alpha proteins hydroxylation.By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD
  • Hsp90 protein binding Source: RGD
  • p53 binding Source: RGD
  • unfolded protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi263737. 1 interaction.
STRINGi10116.ENSRNOP00000003787.

Structurei

3D structure databases

ProteinModelPortaliP83868.
SMRiP83868. Positions 1-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9090CSPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi157 – 1604PXLE motifBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi108 – 16053Asp/Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the p23/wos2 family.Sequence analysis
Contains 1 CS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3158. Eukaryota.
ENOG41121RT. LUCA.
GeneTreeiENSGT00510000046493.
HOGENOMiHOG000177563.
HOVERGENiHBG002143.
InParanoidiP83868.
KOiK15730.
OMAiENESKHK.
OrthoDBiEOG091G0XLQ.
PhylomeDBiP83868.
TreeFamiTF315077.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamiPF04969. CS. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPASAKWYD RRDYVFIEFC VEDSKDVNVN FEKSKLTFSC LGGSDNFKHL
60 70 80 90 100
NEIDLFHCID PNDSKHKRTD RSILCCLRKG ESGQSWPRLT KERAKLNWLS
110 120 130 140 150
VDFNNWKDWE DDSDEDMSNF DRFSEMMDHM GGDEDVDLPE VDGADDDSQD
160
SDDEKMPDLE
Length:160
Mass (Da):18,721
Last modified:June 12, 2007 - v2
Checksum:i7702CB59D7AFD739
GO

Sequence cautioni

The sequence CK475788 differs from that shown. Reason: Frameshift at position 156. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21Q → D AA sequence (PubMed:10922363).Curated
Sequence conflicti37 – 371T → C AA sequence (PubMed:10922363).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CK475788 mRNA. No translation available.
RefSeqiNP_001124461.1. NM_001130989.1.
UniGeneiRn.2344.

Genome annotation databases

EnsembliENSRNOT00000003787; ENSRNOP00000003787; ENSRNOG00000002642.
GeneIDi362809.
KEGGirno:362809.
UCSCiRGD:1561913. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CK475788 mRNA. No translation available.
RefSeqiNP_001124461.1. NM_001130989.1.
UniGeneiRn.2344.

3D structure databases

ProteinModelPortaliP83868.
SMRiP83868. Positions 1-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263737. 1 interaction.
STRINGi10116.ENSRNOP00000003787.

PTM databases

iPTMnetiP83868.
PhosphoSiteiP83868.

Proteomic databases

PaxDbiP83868.
PRIDEiP83868.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003787; ENSRNOP00000003787; ENSRNOG00000002642.
GeneIDi362809.
KEGGirno:362809.
UCSCiRGD:1561913. rat.

Organism-specific databases

CTDi10728.
RGDi1561913. Ptges3.

Phylogenomic databases

eggNOGiKOG3158. Eukaryota.
ENOG41121RT. LUCA.
GeneTreeiENSGT00510000046493.
HOGENOMiHOG000177563.
HOVERGENiHBG002143.
InParanoidiP83868.
KOiK15730.
OMAiENESKHK.
OrthoDBiEOG091G0XLQ.
PhylomeDBiP83868.
TreeFamiTF315077.

Enzyme and pathway databases

UniPathwayiUPA00662.
ReactomeiR-RNO-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-RNO-3371511. HSF1 activation.
R-RNO-3371568. Attenuation phase.
SABIO-RKP83868.

Miscellaneous databases

PROiP83868.

Gene expression databases

ExpressionAtlasiP83868. baseline.
GenevisibleiP83868. RN.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamiPF04969. CS. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTEBP_RAT
AccessioniPrimary (citable) accession number: P83868
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: June 12, 2007
Last modified: September 7, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.