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Protein

Prostaglandin E synthase 3

Gene

Ptges3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) (PubMed:10922363). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway (By similarity).By similarity1 Publication

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate.1 Publication

Pathwayi: prostaglandin biosynthesis

This protein is involved in the pathway prostaglandin biosynthesis, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway prostaglandin biosynthesis and in Lipid metabolism.

GO - Molecular functioni

  • DNA polymerase binding Source: RGD
  • enzyme binding Source: RGD
  • Hsp90 protein binding Source: RGD
  • p53 binding Source: RGD
  • prostaglandin-E synthase activity Source: RGD
  • telomerase activity Source: RGD
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

Enzyme and pathway databases

ReactomeiR-RNO-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-RNO-3371497. HSP90 chaperone cycle for steroid hormone receptors (SHR).
R-RNO-3371511. HSF1 activation.
R-RNO-3371568. Attenuation phase.
R-RNO-8937144. Aryl hydrocarbon receptor signalling.
SABIO-RKiP83868.
UniPathwayiUPA00662.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E synthase 3 (EC:5.3.99.31 Publication)
Alternative name(s):
Cytosolic prostaglandin E2 synthase
Short name:
cPGES
Hsp90 co-chaperone
Progesterone receptor complex p23
Telomerase-binding protein p23
Gene namesi
Name:Ptges3By similarity
Synonyms:TebpBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1561913. Ptges3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002913821 – 160Prostaglandin E synthase 3Add BLAST160

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-acetyllysineBy similarity1
Cross-linki35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei44PhosphoserineBy similarity1
Cross-linki65Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei85PhosphoserineBy similarity1
Modified residuei100PhosphoserineBy similarity1
Modified residuei113PhosphoserineCombined sources1
Modified residuei118PhosphoserineBy similarity1
Modified residuei148PhosphoserineCombined sources1
Modified residuei151PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP83868.
PRIDEiP83868.

PTM databases

iPTMnetiP83868.
PhosphoSitePlusiP83868.

Expressioni

Tissue specificityi

Widely expressed with highest levels in testis.1 Publication

Inductioni

In brain, by LPS.1 Publication

Gene expression databases

BgeeiENSRNOG00000002642.
ExpressionAtlasiP83868. baseline.
GenevisibleiP83868. RN.

Interactioni

Subunit structurei

Binds to the progesterone receptor. Interacts with TERT; the interaction, together with HSP90AA1, is required for correct assembly and stabilization of the telomerase holoenzyme complex. Interacts (via PXLE motif) with EGLN1/PHD2, recruiting EGLN1/PHD2 to the HSP90 pathway to facilitate HIF alpha proteins hydroxylation. Interacts with HSP90AA1, FLCN, FNIP1 and FNIP2.By similarity

GO - Molecular functioni

  • DNA polymerase binding Source: RGD
  • enzyme binding Source: RGD
  • Hsp90 protein binding Source: RGD
  • p53 binding Source: RGD
  • unfolded protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi263737. 1 interactor.
STRINGi10116.ENSRNOP00000003787.

Structurei

3D structure databases

ProteinModelPortaliP83868.
SMRiP83868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 90CSPROSITE-ProRule annotationAdd BLAST90

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi157 – 160PXLE motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi108 – 160Asp/Glu-richAdd BLAST53

Sequence similaritiesi

Belongs to the p23/wos2 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG3158. Eukaryota.
ENOG41121RT. LUCA.
GeneTreeiENSGT00510000046493.
HOGENOMiHOG000177563.
HOVERGENiHBG002143.
InParanoidiP83868.
KOiK15730.
OMAiKAAGPYW.
OrthoDBiEOG091G0XLQ.
PhylomeDBiP83868.
TreeFamiTF315077.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiView protein in InterPro
IPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
PfamiView protein in Pfam
PF04969. CS. 1 hit.
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiView protein in PROSITE
PS51203. CS. 1 hit.

Sequencei

Sequence statusi: Complete.

P83868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPASAKWYD RRDYVFIEFC VEDSKDVNVN FEKSKLTFSC LGGSDNFKHL
60 70 80 90 100
NEIDLFHCID PNDSKHKRTD RSILCCLRKG ESGQSWPRLT KERAKLNWLS
110 120 130 140 150
VDFNNWKDWE DDSDEDMSNF DRFSEMMDHM GGDEDVDLPE VDGADDDSQD
160
SDDEKMPDLE
Length:160
Mass (Da):18,721
Last modified:June 12, 2007 - v2
Checksum:i7702CB59D7AFD739
GO

Sequence cautioni

The sequence CK475788 differs from that shown. Reason: Frameshift at position 156.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2Q → D AA sequence (PubMed:10922363).Curated1
Sequence conflicti37T → C AA sequence (PubMed:10922363).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CK475788 mRNA. No translation available.
RefSeqiNP_001124461.1. NM_001130989.1.
UniGeneiRn.2344.

Genome annotation databases

EnsembliENSRNOT00000003787; ENSRNOP00000003787; ENSRNOG00000002642.
GeneIDi362809.
KEGGirno:362809.
UCSCiRGD:1561913. rat.

Similar proteinsi

Entry informationi

Entry nameiTEBP_RAT
AccessioniPrimary (citable) accession number: P83868
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: June 12, 2007
Last modified: November 22, 2017
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families