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Protein

Inosine-uridine preferring nucleoside hydrolase

Gene

NSNH

Organism
Leishmania major
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of all of the commonly occurring purine and pyrimidine nucleosides into ribose and the associated base, but has a preference for inosine and uridine as substrates.1 Publication

Catalytic activityi

A purine nucleoside + H2O = D-ribose + a purine base.1 Publication

Cofactori

Ca2+1 Publication

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10Calcium1
Binding sitei14SubstrateBy similarity1
Metal bindingi15Calcium1
Metal bindingi126Calcium1
Binding sitei160SubstrateBy similarity1
Binding sitei166SubstrateBy similarity1
Binding sitei168SubstrateBy similarity1
Active sitei240By similarity1
Metal bindingi241Calcium1

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • inosine nucleosidase activity Source: UniProtKB
  • purine nucleosidase activity Source: UniProtKB
  • uridine nucleosidase activity Source: UniProtKB

GO - Biological processi

  • nucleotide metabolic process Source: UniProtKB-KW
  • purine-containing compound salvage Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-uridine preferring nucleoside hydrolase (EC:3.2.2.-)
Short name:
IU-NH
Short name:
IU-nucleoside hydrolase
Alternative name(s):
Non-specific nucleoside hydrolase
Purine nucleosidase
Gene namesi
Name:NSNH
OrganismiLeishmania major
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002068112 – 314Inosine-uridine preferring nucleoside hydrolaseAdd BLAST313

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi5664.LmjF.18.1580.

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi13 – 24Combined sources12
Beta strandi28 – 35Combined sources8
Helixi42 – 55Combined sources14
Beta strandi63 – 65Combined sources3
Beta strandi71 – 73Combined sources3
Beta strandi85 – 87Combined sources3
Helixi105 – 115Combined sources11
Beta strandi121 – 125Combined sources5
Helixi130 – 138Combined sources9
Helixi142 – 145Combined sources4
Beta strandi148 – 152Combined sources5
Beta strandi160 – 164Combined sources5
Helixi167 – 170Combined sources4
Helixi173 – 180Combined sources8
Beta strandi182 – 184Combined sources3
Beta strandi186 – 189Combined sources4
Helixi191 – 194Combined sources4
Helixi201 – 210Combined sources10
Helixi213 – 228Combined sources16
Helixi230 – 232Combined sources3
Beta strandi233 – 235Combined sources3
Helixi242 – 249Combined sources8
Helixi251 – 253Combined sources3
Beta strandi254 – 257Combined sources4
Beta strandi261 – 263Combined sources3
Turni268 – 272Combined sources5
Beta strandi274 – 276Combined sources3
Beta strandi287 – 294Combined sources8
Helixi296 – 310Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EZRX-ray2.50A/B/C/D1-314[»]
ProteinModelPortaliP83851.
SMRiP83851.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83851.

Family & Domainsi

Sequence similaritiesi

Belongs to the IUNH family.1 Publication

Phylogenomic databases

eggNOGiKOG2938. Eukaryota.
COG1957. LUCA.

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83851-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRKIILDCD PGIDDAVAIF LAHGNPEIEL LAITTVVGNQ SLEKVTQNAR
60 70 80 90 100
LVADVAGIVG VPVAAGCTKP LVRGVRNASH IHGETGMGNV SYPPEFKTKL
110 120 130 140 150
DGRHAVQLII DLIMSHEPKT ITLVPTGGLT NIAMAVRLEP RIVDRVKEVV
160 170 180 190 200
LMGGGYHTGN ASPVAEFNVF IDPEAAHIVF NESWNVTMVG LDLTHQALAT
210 220 230 240 250
PAVQKRVREV GTKPAAFMLQ ILDFYTKVYE KEHDTYGKVH DPCAVAYVID
260 270 280 290 300
PTVMTTERVP VDIELNGALT TGMTVVDFRY PRPKNCRTQV AVKLDFDKFW
310
CLVIDALERI GDPQ
Length:314
Mass (Da):34,310
Last modified:January 23, 2007 - v3
Checksum:i947D924C65FD0723
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196Q → L (PubMed:10409664).Curated1
Sequence conflicti276V → A (PubMed:10409664).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY533501 Genomic DNA. Translation: AAS48367.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY533501 Genomic DNA. Translation: AAS48367.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EZRX-ray2.50A/B/C/D1-314[»]
ProteinModelPortaliP83851.
SMRiP83851.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5664.LmjF.18.1580.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG2938. Eukaryota.
COG1957. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00606.

Miscellaneous databases

EvolutionaryTraceiP83851.

Family and domain databases

Gene3Di3.90.245.10. 1 hit.
InterProiIPR015910. I/U_nuclsd_hydro_CS.
IPR023186. Inosine/uridine_hydrolase.
IPR001910. Inosine/uridine_hydrolase_dom.
[Graphical view]
PANTHERiPTHR12304. PTHR12304. 1 hit.
PfamiPF01156. IU_nuc_hydro. 1 hit.
[Graphical view]
SUPFAMiSSF53590. SSF53590. 1 hit.
PROSITEiPS01247. IUNH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIUNH_LEIMA
AccessioniPrimary (citable) accession number: P83851
Secondary accession number(s): Q6QMH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 55 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.