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Protein

ADP-ribose pyrophosphatase

Gene

nudF

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Acts on ADP-mannose and ADP-glucose as well as ADP-ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process (By similarity).By similarity

Catalytic activityi

ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by phosphorylated compounds such as AMP, ADP, ATP, 3-phosphoglyceric acid and PPi. Not inhibited by orthophosphate. Activity is high in cells grown in low glucose concentrations and decreases dramatically as glucose concentration increases (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56SubstrateBy similarity1
Binding sitei79SubstrateBy similarity1
Metal bindingi96Magnesium 1; via carbonyl oxygenBy similarity1
Binding sitei98Substrate; via amide nitrogenBy similarity1
Metal bindingi112Magnesium 2By similarity1
Metal bindingi112Magnesium 3By similarity1
Metal bindingi116Magnesium 1By similarity1
Metal bindingi116Magnesium 3By similarity1
Binding sitei139SubstrateBy similarity1
Active sitei162Proton acceptorBy similarity1
Metal bindingi164Magnesium 3By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribose pyrophosphatase (EC:3.6.1.13)
Alternative name(s):
ADP-ribose diphosphatase
ADP-ribose phosphohydrolase
Short name:
ASPPase
Adenosine diphosphoribose pyrophosphatase
Short name:
ADPR-PPase
Gene namesi
Name:nudF
Synonyms:aspP
Ordered Locus Names:SF3074, S3279
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB02059 Adenosine-5-Diphosphoribose
DB01975 AMPCPR

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000570461 – 209ADP-ribose pyrophosphataseAdd BLAST209

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP83844
SMRiP83844
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 193Nudix hydrolasePROSITE-ProRule annotationAdd BLAST139

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 29Substrate bindingBy similarity2
Regioni51 – 52Substrate binding; shared with dimeric partnerBy similarity2
Regioni133 – 135Substrate binding; shared with dimeric partnerBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi97 – 118Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudF subfamily.Curated

Phylogenomic databases

eggNOGiENOG41087AY Bacteria
ENOG410ZYVG LUCA
HOGENOMiHOG000045857
KOiK01515
OMAiEQAYQWM

Family and domain databases

InterProiView protein in InterPro
IPR004385 NDP_pyrophosphatase
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF00293 NUDIX, 1 hit
SUPFAMiSSF55811 SSF55811, 1 hit
TIGRFAMsiTIGR00052 TIGR00052, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequencei

Sequence statusi: Complete.

P83844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPDNLPVT FGKNDVEIIA RETLYRGFFS LDLYRFRHRL FNGQMSHEVR
60 70 80 90 100
REIFERGHAA VLLPFDPVRD EVVLIEQIRI AAYDTSETPW LLEMVAGMIE
110 120 130 140 150
EGESVEDVAR REAIEEAGLI VKRTKPVLSF LASPGGTSER SSIMVGEVDA
160 170 180 190 200
TTASGIHGLA DENEDIRVHV VSREQAYQWV EEGKIDNAAS VIALQWLQLH

HQALKNEWA
Length:209
Mass (Da):23,667
Last modified:April 13, 2004 - v1
Checksum:i2CF77EA9D63B9615
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN44552.1
AE014073 Genomic DNA Translation: AAP18365.1
RefSeqiNP_708845.1, NC_004337.2
WP_000917117.1, NZ_NIYV01000092.1

Genome annotation databases

EnsemblBacteriaiAAN44552; AAN44552; SF3074
AAP18365; AAP18365; S3279
GeneIDi1026699
KEGGisfl:SF3074
sfx:S3279
PATRICifig|198214.7.peg.3649

Similar proteinsi

Entry informationi

Entry nameiADPP_SHIFL
AccessioniPrimary (citable) accession number: P83844
Secondary accession number(s): P36651
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health