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Protein

ADP-ribose pyrophosphatase

Gene

nudF

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Acts on ADP-mannose and ADP-glucose as well as ADP-ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process (By similarity).By similarity

Catalytic activityi

ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by phosphorylated compounds such as AMP, ADP, ATP, 3-phosphoglyceric acid and PPi. Not inhibited by orthophosphate. Activity is high in cells grown in low glucose concentrations and decreases dramatically as glucose concentration increases (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561SubstrateBy similarity
Binding sitei79 – 791SubstrateBy similarity
Metal bindingi96 – 961Magnesium 1; via carbonyl oxygenBy similarity
Binding sitei98 – 981Substrate; via amide nitrogenBy similarity
Metal bindingi112 – 1121Magnesium 2By similarity
Metal bindingi112 – 1121Magnesium 3By similarity
Metal bindingi116 – 1161Magnesium 1By similarity
Metal bindingi116 – 1161Magnesium 3By similarity
Binding sitei139 – 1391SubstrateBy similarity
Active sitei162 – 1621Proton acceptorBy similarity
Metal bindingi164 – 1641Magnesium 3By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribose pyrophosphatase (EC:3.6.1.13)
Alternative name(s):
ADP-ribose diphosphatase
ADP-ribose phosphohydrolase
Short name:
ASPPase
Adenosine diphosphoribose pyrophosphatase
Short name:
ADPR-PPase
Gene namesi
Name:nudF
Synonyms:aspP
Ordered Locus Names:SF3074, S3279
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209ADP-ribose pyrophosphatasePRO_0000057046Add
BLAST

Proteomic databases

PaxDbiP83844.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi198214.SF3074.

Structurei

3D structure databases

ProteinModelPortaliP83844.
SMRiP83844. Positions 8-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 193139Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni28 – 292Substrate bindingBy similarity
Regioni51 – 522Substrate binding; shared with dimeric partnerBy similarity
Regioni133 – 1353Substrate binding; shared with dimeric partnerBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi97 – 11822Nudix boxAdd
BLAST

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudF subfamily.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41087AY. Bacteria.
ENOG410ZYVG. LUCA.
HOGENOMiHOG000045857.
KOiK01515.
OMAiEQAYQWM.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR004385. NDP_pyrophosphatase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
TIGRFAMsiTIGR00052. TIGR00052. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPDNLPVT FGKNDVEIIA RETLYRGFFS LDLYRFRHRL FNGQMSHEVR
60 70 80 90 100
REIFERGHAA VLLPFDPVRD EVVLIEQIRI AAYDTSETPW LLEMVAGMIE
110 120 130 140 150
EGESVEDVAR REAIEEAGLI VKRTKPVLSF LASPGGTSER SSIMVGEVDA
160 170 180 190 200
TTASGIHGLA DENEDIRVHV VSREQAYQWV EEGKIDNAAS VIALQWLQLH

HQALKNEWA
Length:209
Mass (Da):23,667
Last modified:April 13, 2004 - v1
Checksum:i2CF77EA9D63B9615
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44552.1.
AE014073 Genomic DNA. Translation: AAP18365.1.
RefSeqiNP_708845.1. NC_004337.2.
WP_000917117.1. NZ_LM651928.1.

Genome annotation databases

EnsemblBacteriaiAAN44552; AAN44552; SF3074.
AAP18365; AAP18365; S3279.
GeneIDi1026699.
KEGGisfl:SF3074.
sft:NCTC1_03330.
sfx:S3279.
PATRICi18708276. VBIShiFle31049_3586.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44552.1.
AE014073 Genomic DNA. Translation: AAP18365.1.
RefSeqiNP_708845.1. NC_004337.2.
WP_000917117.1. NZ_LM651928.1.

3D structure databases

ProteinModelPortaliP83844.
SMRiP83844. Positions 8-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF3074.

Proteomic databases

PaxDbiP83844.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN44552; AAN44552; SF3074.
AAP18365; AAP18365; S3279.
GeneIDi1026699.
KEGGisfl:SF3074.
sft:NCTC1_03330.
sfx:S3279.
PATRICi18708276. VBIShiFle31049_3586.

Phylogenomic databases

eggNOGiENOG41087AY. Bacteria.
ENOG410ZYVG. LUCA.
HOGENOMiHOG000045857.
KOiK01515.
OMAiEQAYQWM.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR004385. NDP_pyrophosphatase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
TIGRFAMsiTIGR00052. TIGR00052. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADPP_SHIFL
AccessioniPrimary (citable) accession number: P83844
Secondary accession number(s): P36651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.