Reviewed, UniProtKB/Swiss-Prot P83844 (ADPP_SHIFL)

Names and origin

Protein names

Recommended name:
    ADP-ribose pyrophosphatase
    EC=3.6.1.13
Alternative name(s):
    ADP-ribose diphosphatase
    Adenosine diphosphoribose pyrophosphatase
      Short name=ADPR-PPase
    ADP-ribose phosphohydrolase
      Short name=ASPPase

Gene names

Name:	nudF
Synonyms:	aspP
Ordered Locus Names:	SF3074, S3279

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Acts on ADP-mannose and ADP-glucose as well as ADP-ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process By similarity.
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Enzyme regulation	Inhibited by phosphorylated compounds such as AMP, ADP, ATP, 3-phosphoglyceric acid and PPi. Not inhibited by orthophosphate. Activity is high in cells grown in low glucose concentrations and decreases dramatically as glucose concentration increases By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. NudF subfamily.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ADP-ribose diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	ADP-ribose pyrophosphatase		PRO_0000057046
Regions
	Motif	97 – 118	22	Nudix box
Sites
	Active site	162	1	Proton acceptor By similarity
	Metal binding	112	1	Magnesium 1 By similarity
	Metal binding	112	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 1 By similarity
	Metal binding	116	1	Magnesium 3 By similarity
	Metal binding	164	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P83844-1 [UniParc]. Last modified April 13, 2004. Version 1. Checksum: 2CF77EA9D63B9615	FASTA	209	23,667
	10 20 30 40 50 60 MLKPDNLPVT FGKNDVEIIA RETLYRGFFS LDLYRFRHRL FNGQMSHEVR REIFERGHAA 70 80 90 100 110 120 VLLPFDPVRD EVVLIEQIRI AAYDTSETPW LLEMVAGMIE EGESVEDVAR REAIEEAGLI 130 140 150 160 170 180 VKRTKPVLSF LASPGGTSER SSIMVGEVDA TTASGIHGLA DENEDIRVHV VSREQAYQWV 190 200 EEGKIDNAAS VIALQWLQLH HQALKNEWA

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN44552.1. AE014073 Genomic DNA. Translation: AAP18365.1.
RefSeq	NP_708845.1. NP_838555.1.
3D structure databases
SMR	P83844. Positions 8-209.
ModBase	Search...
Genome annotation databases
GeneID	1026699. 1080971.
GenomeReviews	Gene locus SF3074 in contig AE005674_GR. Gene locus S3279 in contig AE014073_GR.
KEGG	sfl:SF3074. sfx:S3279.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P83844.
OMA	P83844. YFPSPGG.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN44552.1-MON.
BRENDA	3.6.1.13. 189495.
Family and domain databases
InterPro	IPR004385. NDP_pyrophos. IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
TIGRFAMs	TIGR00052. NDP_pyrophos. 1 hit.
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADPP_SHIFL

Accession

Primary (citable) accession number: P83844
Secondary accession number(s): P36651

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)