Reviewed, UniProtKB/Swiss-Prot P83842 (ADPP_ECOL6)

Names and origin

Protein names

Recommended name:
    ADP-ribose pyrophosphatase
    EC=3.6.1.13
Alternative name(s):
    ADP-ribose diphosphatase
    Adenosine diphosphoribose pyrophosphatase
      Short name=ADPR-PPase
    ADP-ribose phosphohydrolase
      Short name=ASPPase

Gene names

Name:	nudF
Synonyms:	aspP
Ordered Locus Names:	c3780

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Acts on ADP-mannose and ADP-glucose as well as ADP-ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process By similarity.
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Enzyme regulation	Inhibited by phosphorylated compounds such as AMP, ADP, ATP, 3-phosphoglyceric acid and PPi. Not inhibited by orthophosphate. Activity is high in cells grown in low glucose concentrations and decreases dramatically as glucose concentration increases By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. NudF subfamily.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ADP-ribose diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	ADP-ribose pyrophosphatase		PRO_0000057043
Regions
	Motif	97 – 118	22	Nudix box
Sites
	Active site	162	1	Proton acceptor By similarity
	Metal binding	112	1	Magnesium 1 By similarity
	Metal binding	112	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 1 By similarity
	Metal binding	116	1	Magnesium 3 By similarity
	Metal binding	164	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P83842-1 [UniParc]. Last modified April 13, 2004. Version 1. Checksum: 2CF77EA9D63B9615	FASTA	209	23,667
	10 20 30 40 50 60 MLKPDNLPVT FGKNDVEIIA RETLYRGFFS LDLYRFRHRL FNGQMSHEVR REIFERGHAA 70 80 90 100 110 120 VLLPFDPVRD EVVLIEQIRI AAYDTSETPW LLEMVAGMIE EGESVEDVAR REAIEEAGLI 130 140 150 160 170 180 VKRTKPVLSF LASPGGTSER SSIMVGEVDA TTASGIHGLA DENEDIRVHV VSREQAYQWV 190 200 EEGKIDNAAS VIALQWLQLH HQALKNEWA

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014075 Genomic DNA. Translation: AAN82224.1.
RefSeq	NP_755651.1.
3D structure databases
SMR	P83842. Positions 8-209.
ModBase	Search...
Genome annotation databases
GeneID	1039920.
GenomeReviews	Gene locus c3780 in contig AE014075_GR.
KEGG	ecc:c3780.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P83842.
OMA	P83842. YFPSPGG.
Enzyme and pathway databases
BRENDA	3.6.1.13. 292881.
Family and domain databases
InterPro	IPR004385. NDP_pyrophos. IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
TIGRFAMs	TIGR00052. NDP_pyrophos. 1 hit.
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADPP_ECOL6

Accession

Primary (citable) accession number: P83842
Secondary accession number(s): P36651

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)