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P83794

- UCRI_MASLA

UniProt

P83794 - UCRI_MASLA

Protein

Cytochrome b6-f complex iron-sulfur subunit

Gene

petC

Organism
Mastigocladus laminosus (Fischerella sp.)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

    Catalytic activityi

    Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi108 – 1081Iron-sulfur (2Fe-2S)
    Metal bindingi110 – 1101Iron-sulfur (2Fe-2S); via pros nitrogen
    Metal bindingi126 – 1261Iron-sulfur (2Fe-2S)
    Metal bindingi129 – 1291Iron-sulfur (2Fe-2S); via pros nitrogen

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. plastoquinol--plastocyanin reductase activity Source: UniProtKB-HAMAP
    5. ubiquinol-cytochrome-c reductase activity Source: InterPro

    GO - Biological processi

    1. photosynthesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit (EC:1.10.9.1)
    Alternative name(s):
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
    Short name:
    ISP
    Short name:
    RISP
    Rieske iron-sulfur protein
    Gene namesi
    Name:petC
    OrganismiMastigocladus laminosus (Fischerella sp.)
    Taxonomic identifieri83541 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaStigonematalesMastigocladus

    Subcellular locationi

    Cellular thylakoid membrane By similarity; Single-pass membrane protein By similarity
    Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. thylakoid membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 179179Cytochrome b6-f complex iron-sulfur subunitPRO_0000127775Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi113 ↔ 128

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.

    Structurei

    Secondary structure

    1
    179
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 4230
    Beta strandi59 – 613
    Helixi66 – 716
    Beta strandi80 – 823
    Beta strandi84 – 874
    Beta strandi88 – 914
    Beta strandi97 – 993
    Beta strandi101 – 1055
    Turni109 – 1113
    Beta strandi119 – 1257
    Turni127 – 1293
    Beta strandi131 – 1333
    Beta strandi135 – 1373
    Beta strandi141 – 1433
    Beta strandi150 – 1545
    Beta strandi156 – 1594
    Beta strandi161 – 1644
    Turni170 – 1723

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VF5X-ray3.00D/Q1-179[»]
    2D2CX-ray3.80D/Q1-179[»]
    2E74X-ray3.00D1-179[»]
    2E75X-ray3.55D1-179[»]
    2E76X-ray3.41D1-179[»]
    4H0LX-ray3.25D1-179[»]
    4H13X-ray3.07D1-179[»]
    4I7ZX-ray2.80D1-179[»]
    ProteinModelPortaliP83794.
    SMRiP83794. Positions 9-179.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP83794.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4323HelicalAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini61 – 162102RieskeAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Rieske domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.102.10.10. 1 hit.
    HAMAPiMF_01335. Cytb6_f_Rieske.
    InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
    IPR023960. Cyt_b6_f_Rieske.
    IPR017941. Rieske_2Fe-2S.
    IPR014349. Rieske_Fe-S_prot.
    IPR005805. Rieske_Fe-S_prot_C.
    [Graphical view]
    PANTHERiPTHR10134. PTHR10134. 1 hit.
    PfamiPF08802. CytB6-F_Fe-S. 1 hit.
    PF00355. Rieske. 1 hit.
    [Graphical view]
    PRINTSiPR00162. RIESKE.
    SUPFAMiSSF50022. SSF50022. 1 hit.
    PROSITEiPS51296. RIESKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P83794-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQFTESMDV PDMGRRQFMN LLAFGTVTGV ALGALYPLVK YFIPPSGGAV    50
    GGGTTAKDKL GNNVKVSKFL ESHNAGDRVL VQGLKGDPTY IVVESKEAIR 100
    DYGINAVCTH LGCVVPWNAA ENKFKCPCHG SQYDETGKVI RGPAPLSLAL 150
    CHATVQDDNI VLTPWTETDF RTGEKPWWV 179
    Length:179
    Mass (Da):19,401
    Last modified:June 21, 2005 - v2
    Checksum:i3797DC66C5AFAD6C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti138 – 1381K → R(PubMed:14526088)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY390356 Genomic DNA. Translation: AAR26240.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY390356 Genomic DNA. Translation: AAR26240.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VF5 X-ray 3.00 D/Q 1-179 [» ]
    2D2C X-ray 3.80 D/Q 1-179 [» ]
    2E74 X-ray 3.00 D 1-179 [» ]
    2E75 X-ray 3.55 D 1-179 [» ]
    2E76 X-ray 3.41 D 1-179 [» ]
    4H0L X-ray 3.25 D 1-179 [» ]
    4H13 X-ray 3.07 D 1-179 [» ]
    4I7Z X-ray 2.80 D 1-179 [» ]
    ProteinModelPortali P83794.
    SMRi P83794. Positions 9-179.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P83794.

    Family and domain databases

    Gene3Di 2.102.10.10. 1 hit.
    HAMAPi MF_01335. Cytb6_f_Rieske.
    InterProi IPR014909. Cyt_b6-f_cplx_Fe-S_su.
    IPR023960. Cyt_b6_f_Rieske.
    IPR017941. Rieske_2Fe-2S.
    IPR014349. Rieske_Fe-S_prot.
    IPR005805. Rieske_Fe-S_prot_C.
    [Graphical view ]
    PANTHERi PTHR10134. PTHR10134. 1 hit.
    Pfami PF08802. CytB6-F_Fe-S. 1 hit.
    PF00355. Rieske. 1 hit.
    [Graphical view ]
    PRINTSi PR00162. RIESKE.
    SUPFAMi SSF50022. SSF50022. 1 hit.
    PROSITEi PS51296. RIESKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the petBD and petCA operon from the thermophilic cyanobacterium Mastigocladus laminosus."
      Yan J., Zhang H., Cramer W.A.
      Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity."
      Kurisu G., Zhang H., Smith J.L., Cramer W.A.
      Science 302:1009-1014(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

    Entry informationi

    Entry nameiUCRI_MASLA
    AccessioniPrimary (citable) accession number: P83794
    Secondary accession number(s): Q5YJJ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3