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P83794 (UCRI_MASLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome b6-f complex iron-sulfur subunit
EC=1.10.9.1
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Short name=ISP
Short name=RISP
Rieske iron-sulfur protein
Gene names
Name:petC
OrganismMastigocladus laminosus (Fischerella sp.)
Taxonomic identifier83541 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Stigonematales › Mastigocladus

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. HAMAP-Rule MF_01335

Catalytic activity

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 2 H+(Side 2). HAMAP-Rule MF_01335

Cofactor

Binds 1 2Fe-2S cluster per subunit.

Subunit structure

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.

Subcellular location

Cellular thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer. HAMAP-Rule MF_01335

Miscellaneous

The Rieske iron-sulfur protein is a high potential 2Fe-2S protein. HAMAP-Rule MF_01335

Sequence similarities

Contains 1 Rieske domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179Cytochrome b6-f complex iron-sulfur subunit HAMAP-Rule MF_01335
PRO_0000127775

Regions

Transmembrane21 – 4323Helical HAMAP-Rule MF_01335
Domain61 – 162102Rieske

Sites

Metal binding1081Iron-sulfur (2Fe-2S)
Metal binding1101Iron-sulfur (2Fe-2S); via pros nitrogen
Metal binding1261Iron-sulfur (2Fe-2S)
Metal binding1291Iron-sulfur (2Fe-2S); via pros nitrogen

Amino acid modifications

Disulfide bond113 ↔ 128 HAMAP-Rule MF_01335

Experimental info

Sequence conflict1381K → R Ref.2

Secondary structure

1 ...................................... 179
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P83794 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: 3797DC66C5AFAD6C

FASTA17919,401
        10         20         30         40         50         60 
MAQFTESMDV PDMGRRQFMN LLAFGTVTGV ALGALYPLVK YFIPPSGGAV GGGTTAKDKL 

        70         80         90        100        110        120 
GNNVKVSKFL ESHNAGDRVL VQGLKGDPTY IVVESKEAIR DYGINAVCTH LGCVVPWNAA 

       130        140        150        160        170 
ENKFKCPCHG SQYDETGKVI RGPAPLSLAL CHATVQDDNI VLTPWTETDF RTGEKPWWV

« Hide

References

[1]"Cloning and characterization of the petBD and petCA operon from the thermophilic cyanobacterium Mastigocladus laminosus."
Yan J., Zhang H., Cramer W.A.
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity."
Kurisu G., Zhang H., Smith J.L., Cramer W.A.
Science 302:1009-1014(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY390356 Genomic DNA. Translation: AAR26240.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VF5X-ray3.00D/Q1-179[»]
2D2CX-ray3.80D/Q1-179[»]
2E74X-ray3.00D1-179[»]
2E75X-ray3.55D1-179[»]
2E76X-ray3.41D1-179[»]
4H0LX-ray3.25D1-179[»]
4H13X-ray3.07D1-179[»]
ProteinModelPortalP83794.
SMRP83794. Positions 9-179.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.102.10.10. 1 hit.
HAMAPMF_01335. Cytb6_f_Rieske.
InterProIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERPTHR10134. PTHR10134. 1 hit.
PfamPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSPR00162. RIESKE.
SUPFAMSSF50022. Rieske_dom. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP83794.

Entry information

Entry nameUCRI_MASLA
AccessionPrimary (citable) accession number: P83794
Secondary accession number(s): Q5YJJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 21, 2005
Last modified: April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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