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P83794

- UCRI_MASLA

UniProt

P83794 - UCRI_MASLA

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Protein

Cytochrome b6-f complex iron-sulfur subunit

Gene

petC

Organism
Mastigocladus laminosus (Fischerella sp.)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Catalytic activityi

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081Iron-sulfur (2Fe-2S)
Metal bindingi110 – 1101Iron-sulfur (2Fe-2S); via pros nitrogen
Metal bindingi126 – 1261Iron-sulfur (2Fe-2S)
Metal bindingi129 – 1291Iron-sulfur (2Fe-2S); via pros nitrogen

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. plastoquinol--plastocyanin reductase activity Source: UniProtKB-HAMAP
  5. ubiquinol-cytochrome-c reductase activity Source: InterPro

GO - Biological processi

  1. photosynthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6-f complex iron-sulfur subunit (EC:1.10.9.1)
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Short name:
ISP
Short name:
RISP
Rieske iron-sulfur protein
Gene namesi
Name:petC
OrganismiMastigocladus laminosus (Fischerella sp.)
Taxonomic identifieri83541 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaStigonematalesMastigocladus

Subcellular locationi

Cellular thylakoid membrane By similarity; Single-pass membrane protein By similarity
Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4323HelicalAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. thylakoid membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Cytochrome b6-f complex iron-sulfur subunitPRO_0000127775Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi113 ↔ 128

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.

Structurei

Secondary structure

1
179
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 4230Combined sources
Beta strandi59 – 624Combined sources
Helixi66 – 716Combined sources
Beta strandi80 – 823Combined sources
Beta strandi84 – 874Combined sources
Beta strandi88 – 914Combined sources
Beta strandi97 – 993Combined sources
Beta strandi101 – 1055Combined sources
Turni109 – 1113Combined sources
Beta strandi119 – 1257Combined sources
Turni127 – 1293Combined sources
Beta strandi131 – 1333Combined sources
Beta strandi135 – 1373Combined sources
Beta strandi141 – 1433Combined sources
Beta strandi150 – 1545Combined sources
Beta strandi156 – 1594Combined sources
Beta strandi161 – 1644Combined sources
Turni170 – 1723Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VF5X-ray3.00D/Q1-179[»]
2D2CX-ray3.80D/Q1-179[»]
2E74X-ray3.00D1-179[»]
2E75X-ray3.55D1-179[»]
2E76X-ray3.41D1-179[»]
4H0LX-ray3.25D1-179[»]
4H13X-ray3.07D1-179[»]
4I7ZX-ray2.80D1-179[»]
4PV1X-ray3.00D1-179[»]
ProteinModelPortaliP83794.
SMRiP83794. Positions 9-179.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83794.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 162102RieskeAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01335. Cytb6_f_Rieske.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83794-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQFTESMDV PDMGRRQFMN LLAFGTVTGV ALGALYPLVK YFIPPSGGAV
60 70 80 90 100
GGGTTAKDKL GNNVKVSKFL ESHNAGDRVL VQGLKGDPTY IVVESKEAIR
110 120 130 140 150
DYGINAVCTH LGCVVPWNAA ENKFKCPCHG SQYDETGKVI RGPAPLSLAL
160 170
CHATVQDDNI VLTPWTETDF RTGEKPWWV
Length:179
Mass (Da):19,401
Last modified:June 21, 2005 - v2
Checksum:i3797DC66C5AFAD6C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti138 – 1381K → R(PubMed:14526088)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390356 Genomic DNA. Translation: AAR26240.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390356 Genomic DNA. Translation: AAR26240.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VF5 X-ray 3.00 D/Q 1-179 [» ]
2D2C X-ray 3.80 D/Q 1-179 [» ]
2E74 X-ray 3.00 D 1-179 [» ]
2E75 X-ray 3.55 D 1-179 [» ]
2E76 X-ray 3.41 D 1-179 [» ]
4H0L X-ray 3.25 D 1-179 [» ]
4H13 X-ray 3.07 D 1-179 [» ]
4I7Z X-ray 2.80 D 1-179 [» ]
4PV1 X-ray 3.00 D 1-179 [» ]
ProteinModelPortali P83794.
SMRi P83794. Positions 9-179.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P83794.

Family and domain databases

Gene3Di 2.102.10.10. 1 hit.
HAMAPi MF_01335. Cytb6_f_Rieske.
InterProi IPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view ]
PANTHERi PTHR10134. PTHR10134. 1 hit.
Pfami PF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view ]
PRINTSi PR00162. RIESKE.
SUPFAMi SSF50022. SSF50022. 1 hit.
PROSITEi PS51296. RIESKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the petBD and petCA operon from the thermophilic cyanobacterium Mastigocladus laminosus."
    Yan J., Zhang H., Cramer W.A.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity."
    Kurisu G., Zhang H., Smith J.L., Cramer W.A.
    Science 302:1009-1014(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

Entry informationi

Entry nameiUCRI_MASLA
AccessioniPrimary (citable) accession number: P83794
Secondary accession number(s): Q5YJJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 21, 2005
Last modified: November 26, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3