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Protein

Cytochrome f

Gene

petA

Organism
Mastigocladus laminosus (Fischerella sp.)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.1 Publication

Cofactori

heme1 PublicationNote: Binds 1 heme group covalently.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Iron (heme axial ligand); via amino nitrogen1 Publication
Binding sitei66 – 661Heme (covalent)1 Publication
Binding sitei69 – 691Heme (covalent)1 Publication
Metal bindingi70 – 701Iron (heme axial ligand)1 Publication

GO - Molecular functioni

  1. electron carrier activity Source: UniProtKB-HAMAP
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-HAMAP
  2. photosynthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome f
Gene namesi
Name:petA
OrganismiMastigocladus laminosus (Fischerella sp.)
Taxonomic identifieri83541 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaStigonematalesMastigocladus

Subcellular locationi

  1. Cellular thylakoid membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei299 – 31921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of thylakoid membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Sequence AnalysisAdd
BLAST
Chaini38 – 333296Cytochrome fPRO_0000207977Add
BLAST

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.1 Publication

Structurei

Secondary structure

1
333
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi46 – 527Combined sources
Beta strandi53 – 553Combined sources
Helixi65 – 684Combined sources
Beta strandi77 – 793Combined sources
Beta strandi82 – 843Combined sources
Beta strandi89 – 957Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi115 – 1217Combined sources
Helixi131 – 1333Combined sources
Helixi136 – 1427Combined sources
Beta strandi148 – 1514Combined sources
Beta strandi157 – 1648Combined sources
Turni165 – 1673Combined sources
Beta strandi169 – 1768Combined sources
Turni180 – 1823Combined sources
Beta strandi189 – 20012Combined sources
Beta strandi212 – 2143Combined sources
Beta strandi219 – 2224Combined sources
Beta strandi227 – 2304Combined sources
Beta strandi233 – 2353Combined sources
Beta strandi238 – 2425Combined sources
Beta strandi245 – 2484Combined sources
Beta strandi252 – 2543Combined sources
Beta strandi255 – 2595Combined sources
Beta strandi283 – 29311Combined sources
Helixi296 – 32833Combined sources
Turni329 – 3313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VF5X-ray3.00C/P45-333[»]
2D2CX-ray3.80C/P45-333[»]
2E74X-ray3.00C45-333[»]
2E75X-ray3.55C45-333[»]
2E76X-ray3.41C45-333[»]
4H0LX-ray3.25C45-333[»]
4H13X-ray3.07C45-333[»]
4I7ZX-ray2.80C45-333[»]
4PV1X-ray3.00C45-333[»]
ProteinModelPortaliP83793.
SMRiP83793. Positions 45-332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83793.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome f family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.5.700. 1 hit.
2.60.40.830. 2 hits.
HAMAPiMF_00610. Cytb6_f_cytF.
InterProiIPR024058. Cyt-f_TM.
IPR002325. Cyt_f.
IPR024094. Cyt_f_lg_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01333. Apocytochr_F_C. 1 hit.
[Graphical view]
PRINTSiPR00610. CYTOCHROMEF.
SUPFAMiSSF103431. SSF103431. 1 hit.
SSF49441. SSF49441. 2 hits.
SSF51246. SSF51246. 1 hit.
PROSITEiPS51010. CYTF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNSCKKARR TRPLKATIQA LLVAIATMTF FFTSDIALPQ SAAAYPFWAQ
60 70 80 90 100
QTYPETPREP TGRIVCANCH LAAKPAEVEV PQSVLPDTVF KAVVKIPYDT
110 120 130 140 150
KLQQVAADGS KVGLNVGAVL MLPEGFKIAP EERIPEELKK EVGDVYFQPY
160 170 180 190 200
KEGQDNVLLV GPLPGEQYQE IVFPVLSPNP TTDKNIHFGK YAIHLGANRG
210 220 230 240 250
RGQIYPTGEK SNNNVFTASA TGTITKIAKE EDEYGNVKYQ VSIQTDSGKT
260 270 280 290 300
VVDTIPAGPE LIVSEGQAVK AGEALTNNPN VGGFGQDDTE IVLQDPNRVK
310 320 330
WMIAFICLVM LAQLMLILKK KQVEKVQAAE MNF
Length:333
Mass (Da):36,450
Last modified:July 1, 2008 - v2
Checksum:iE768F5C264323061
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390356 Genomic DNA. Translation: AAR26241.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390356 Genomic DNA. Translation: AAR26241.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VF5X-ray3.00C/P45-333[»]
2D2CX-ray3.80C/P45-333[»]
2E74X-ray3.00C45-333[»]
2E75X-ray3.55C45-333[»]
2E76X-ray3.41C45-333[»]
4H0LX-ray3.25C45-333[»]
4H13X-ray3.07C45-333[»]
4I7ZX-ray2.80C45-333[»]
4PV1X-ray3.00C45-333[»]
ProteinModelPortaliP83793.
SMRiP83793. Positions 45-332.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP83793.

Family and domain databases

Gene3Di1.20.5.700. 1 hit.
2.60.40.830. 2 hits.
HAMAPiMF_00610. Cytb6_f_cytF.
InterProiIPR024058. Cyt-f_TM.
IPR002325. Cyt_f.
IPR024094. Cyt_f_lg_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01333. Apocytochr_F_C. 1 hit.
[Graphical view]
PRINTSiPR00610. CYTOCHROMEF.
SUPFAMiSSF103431. SSF103431. 1 hit.
SSF49441. SSF49441. 2 hits.
SSF51246. SSF51246. 1 hit.
PROSITEiPS51010. CYTF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the petBD and petCA operon from the thermophilic cyanobacterium Mastigocladus laminosus."
    Yan J., Zhang H., Cramer W.A.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity."
    Kurisu G., Zhang H., Smith J.L., Cramer W.A.
    Science 302:1009-1014(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCYF_MASLA
AccessioniPrimary (citable) accession number: P83793
Secondary accession number(s): Q5YJJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: July 1, 2008
Last modified: April 1, 2015
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.