P83793 (CYF_MASLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apocytochrome f

Gene names

Name:

petA

Organism

Mastigocladus laminosus (Fischerella sp.)

Taxonomic identifier

83541 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Stigonematales › Mastigocladus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. HAMAP-Rule MF_00610
Cofactor	Binds 1 heme group covalently.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.
Subcellular location	Cellular thylakoid membrane; Single-pass membrane protein HAMAP-Rule MF_00610.
Sequence similarities	Belongs to the cytochrome f family.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Membrane Thylakoid
Domain	Signal Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 37

37

Potential

Chain

38 – 333

296

Apocytochrome f HAMAP-Rule MF_00610

PRO_0000207977

Regions

Transmembrane

299 – 319

21

Helical; Potential

Sites

Metal binding

45

1

Iron (heme axial ligand); via amino nitrogen By similarity

Metal binding

70

1

Iron (heme axial ligand) By similarity

Binding site

66

1

Heme (covalent) By similarity

Binding site

69

1

Heme (covalent) By similarity

Secondary structure

1

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333

Helix Strand Turn

Details...

Helix

46 – 52

7

Beta strand

53 – 55

3

Helix

65 – 68

4

Beta strand

77 – 79

3

Beta strand

82 – 84

3

Beta strand

89 – 95

7

Beta strand

107 – 109

3

Beta strand

119 – 121

3

Turn

131 – 133

3

Helix

137 – 140

4

Beta strand

148 – 151

4

Beta strand

155 – 159

5

Turn

165 – 167

3

Beta strand

172 – 176

5

Turn

180 – 182

3

Beta strand

189 – 200

12

Beta strand

212 – 214

3

Beta strand

219 – 222

4

Beta strand

227 – 230

4

Beta strand

233 – 235

3

Beta strand

238 – 242

5

Beta strand

247 – 249

3

Beta strand

252 – 254

3

Beta strand

257 – 259

3

Beta strand

284 – 293

10

Helix

296 – 319

24

Helix

321 – 323

3

Helix

327 – 329

3

Sequences

	Sequence		Length	Mass (Da)
	P83793 [UniParc]. Last modified July 1, 2008. Version 2. Checksum: E768F5C264323061	FASTA	333	36,450
	10 20 30 40 50 60 MRNSCKKARR TRPLKATIQA LLVAIATMTF FFTSDIALPQ SAAAYPFWAQ QTYPETPREP 70 80 90 100 110 120 TGRIVCANCH LAAKPAEVEV PQSVLPDTVF KAVVKIPYDT KLQQVAADGS KVGLNVGAVL 130 140 150 160 170 180 MLPEGFKIAP EERIPEELKK EVGDVYFQPY KEGQDNVLLV GPLPGEQYQE IVFPVLSPNP 190 200 210 220 230 240 TTDKNIHFGK YAIHLGANRG RGQIYPTGEK SNNNVFTASA TGTITKIAKE EDEYGNVKYQ 250 260 270 280 290 300 VSIQTDSGKT VVDTIPAGPE LIVSEGQAVK AGEALTNNPN VGGFGQDDTE IVLQDPNRVK 310 320 330 WMIAFICLVM LAQLMLILKK KQVEKVQAAE MNF

References

[1]	"Cloning and characterization of the petBD and petCA operon from the thermophilic cyanobacterium Mastigocladus laminosus." Yan J., Zhang H., Cramer W.A. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity." Kurisu G., Zhang H., Smith J.L., Cramer W.A. Science 302:1009-1014(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY390356 Genomic DNA. Translation: AAR26241.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VF5	X-ray	3.00	C/P	45-333	[»]
2D2C	X-ray	3.80	C/P	45-333	[»]
2E74	X-ray	3.00	C	45-333	[»]
2E75	X-ray	3.55	C	45-333	[»]
2E76	X-ray	3.41	C	45-333	[»]
4H0L	X-ray	3.25	C	45-333	[»]
4H13	X-ray	3.07	C	45-333	[»]

ProteinModelPortal

P83793.

SMR

P83793. Positions 45-332.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.20.5.700. 1 hit.
2.60.40.830. 2 hits.

HAMAP

MF_00610. Cytb6_f_cytF.

InterPro

IPR024058. Cyt-f_TM.
IPR002325. Cyt_f.
IPR024094. Cyt_f_lg_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]

Pfam

PF01333. Apocytochr_F_C. 1 hit.
[Graphical view]

PRINTS

PR00610. CYTOCHROMEF.

SUPFAM

SSF49441. Apocyt_F. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
SSF103431. SSF103431. 1 hit.

PROSITE

PS51010. CYTF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P83793.

Entry information

Entry name

CYF_MASLA

Accession

Primary (citable) accession number: P83793
Secondary accession number(s): Q5YJJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	July 1, 2008
Last modified:	April 3, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program