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Protein

Cytochrome b6

Gene

petB

Organism
Mastigocladus laminosus (Fischerella sp.)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.1 Publication

Cofactori

heme2 PublicationsNote: Binds 2 heme groups. One heme group is bound covalently by a single cysteine link, the other one non-covalently.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35Heme 1 (covalent; via 1 link)1 Publication1
Metal bindingi86Iron (heme 2 axial ligand); via tele nitrogen1 Publication1
Metal bindingi100Iron (heme 1 axial ligand); via tele nitrogen1 Publication1
Metal bindingi187Iron (heme 2 axial ligand)1 Publication1
Metal bindingi202Iron (heme 1 axial ligand)1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.10.9.1. 7666.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6UniRule annotation
Gene namesi
Name:petBUniRule annotation
OrganismiMastigocladus laminosus (Fischerella sp.)
Taxonomic identifieri83541 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesHapalosiphonaceaeMastigocladus

Subcellular locationi

  • Cellular thylakoid membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31Cytoplasmic1 PublicationAdd BLAST31
Transmembranei32 – 52HelicalUniRule annotation1 PublicationAdd BLAST21
Topological domaini53 – 89Lumenal, thylakoid1 PublicationAdd BLAST37
Transmembranei90 – 110HelicalUniRule annotation1 PublicationAdd BLAST21
Topological domaini111 – 115Cytoplasmic1 Publication5
Transmembranei116 – 136HelicalUniRule annotation1 PublicationAdd BLAST21
Topological domaini137 – 185Lumenal, thylakoid1 PublicationAdd BLAST49
Transmembranei186 – 206HelicalUniRule annotation1 PublicationAdd BLAST21
Topological domaini207 – 215Cytoplasmic1 Publication9

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000618271 – 215Cytochrome b6Add BLAST215

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.1 Publication

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 12Combined sources9
Helixi15 – 23Combined sources9
Helixi32 – 35Combined sources4
Helixi36 – 54Combined sources19
Turni55 – 57Combined sources3
Turni62 – 64Combined sources3
Helixi65 – 74Combined sources10
Helixi79 – 105Combined sources27
Turni106 – 110Combined sources5
Helixi112 – 114Combined sources3
Helixi115 – 136Combined sources22
Turni137 – 139Combined sources3
Helixi142 – 151Combined sources10
Helixi152 – 157Combined sources6
Turni159 – 161Combined sources3
Helixi162 – 170Combined sources9
Beta strandi172 – 174Combined sources3
Helixi177 – 188Combined sources12
Helixi190 – 209Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VF5X-ray3.00A/N1-215[»]
2D2CX-ray3.80A/N1-215[»]
2E74X-ray3.00A1-215[»]
2E75X-ray3.55A1-215[»]
2E76X-ray3.41A1-215[»]
4H0LX-ray3.25A1-215[»]
4H13X-ray3.07A1-215[»]
4I7ZX-ray2.80A1-215[»]
4PV1X-ray3.00A1-215[»]
ProteinModelPortaliP83791.
SMRiP83791.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83791.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family. PetB subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd00284. Cytochrome_b_N. 1 hit.
Gene3Di1.20.810.10. 1 hit.
HAMAPiMF_00633. Cytb6_f_cytb6. 1 hit.
InterProiIPR005797. Cyt_b/b6_N.
IPR023530. Cyt_B6_PetB.
IPR027387. Cytb/b6-like.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF000032. Cytochrome_b6. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
PROSITEiPS51002. CYTB_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANVYDWFQE RLEIQALADD VTSKYVPPHV NIFYCLGGIT LTCFLIQFAT
60 70 80 90 100
GFAMTFYYKP TVTEAYASVQ YIMNEVSFGW LIRSIHRWSA SMMVLMMILH
110 120 130 140 150
VFRVYLTGGF KKPRELTWIS GVILAVITVS FGVTGYSLPW DQVGYWAVKI
160 170 180 190 200
VSGVPEAIPV VGVLISDLLR GGSSVGQATL TRYYSAHTFV LPWLIAVFML
210
LHFLMIRKQG ISGPL
Length:215
Mass (Da):24,227
Last modified:March 15, 2004 - v1
Checksum:i4FDCD9336CD31642
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VF5X-ray3.00A/N1-215[»]
2D2CX-ray3.80A/N1-215[»]
2E74X-ray3.00A1-215[»]
2E75X-ray3.55A1-215[»]
2E76X-ray3.41A1-215[»]
4H0LX-ray3.25A1-215[»]
4H13X-ray3.07A1-215[»]
4I7ZX-ray2.80A1-215[»]
4PV1X-ray3.00A1-215[»]
ProteinModelPortaliP83791.
SMRiP83791.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.10.9.1. 7666.

Miscellaneous databases

EvolutionaryTraceiP83791.

Family and domain databases

CDDicd00284. Cytochrome_b_N. 1 hit.
Gene3Di1.20.810.10. 1 hit.
HAMAPiMF_00633. Cytb6_f_cytb6. 1 hit.
InterProiIPR005797. Cyt_b/b6_N.
IPR023530. Cyt_B6_PetB.
IPR027387. Cytb/b6-like.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF000032. Cytochrome_b6. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
PROSITEiPS51002. CYTB_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB6_MASLA
AccessioniPrimary (citable) accession number: P83791
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 15, 2004
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Heme 1 (or BH or b566) is high-potential and absorbs at about 566 nm, and heme 2 (or BL or b562) is low-potential and absorbs at about 562 nm.UniRule annotation

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.