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P83791

- CYB6_MASLA

UniProt

P83791 - CYB6_MASLA

Protein

Cytochrome b6

Gene

petB

Organism
Mastigocladus laminosus (Fischerella sp.)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (15 Mar 2004)
      Previous versions | rss
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    Functioni

    Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

    Cofactori

    Binds 2 heme groups. One heme group is bound covalently by a single cysteine link, the other one non-covalently.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei35 – 351Heme 1 (covalent; via 1 link)
    Metal bindingi86 – 861Iron (heme 2 axial ligand)
    Metal bindingi100 – 1001Iron (heme 1 axial ligand)
    Metal bindingi187 – 1871Iron (heme 2 axial ligand)
    Metal bindingi202 – 2021Iron (heme 1 axial ligand)

    GO - Molecular functioni

    1. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: UniProtKB-HAMAP
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. photosynthesis Source: UniProtKB-HAMAP
    2. respiratory electron transport chain Source: InterPro

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b6UniRule annotation
    Gene namesi
    Name:petBUniRule annotation
    OrganismiMastigocladus laminosus (Fischerella sp.)
    Taxonomic identifieri83541 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaStigonematalesMastigocladus

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. thylakoid membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 215215Cytochrome b6PRO_0000061827Add
    BLAST

    Interactioni

    Subunit structurei

    The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.

    Structurei

    Secondary structure

    1
    215
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 129
    Helixi15 – 239
    Helixi32 – 354
    Helixi36 – 5419
    Turni55 – 573
    Turni62 – 643
    Helixi65 – 7410
    Helixi79 – 10527
    Turni106 – 1105
    Helixi112 – 1143
    Helixi115 – 13622
    Turni137 – 1393
    Helixi142 – 15110
    Helixi152 – 1576
    Turni159 – 1613
    Helixi162 – 1709
    Beta strandi172 – 1743
    Helixi177 – 18812
    Helixi190 – 20920

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VF5X-ray3.00A/N1-215[»]
    2D2CX-ray3.80A/N1-215[»]
    2E74X-ray3.00A1-215[»]
    2E75X-ray3.55A1-215[»]
    2E76X-ray3.41A1-215[»]
    4H0LX-ray3.25A1-215[»]
    4H13X-ray3.07A1-215[»]
    4I7ZX-ray2.80A1-215[»]
    ProteinModelPortaliP83791.
    SMRiP83791. Positions 1-215.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP83791.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5221HelicalUniRule annotationAdd
    BLAST
    Transmembranei90 – 11021HelicalUniRule annotationAdd
    BLAST
    Transmembranei116 – 13621HelicalUniRule annotationAdd
    BLAST
    Transmembranei186 – 20621HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome b family. PetB subfamily.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.810.10. 1 hit.
    HAMAPiMF_00633. Cytb6_f_cytb6.
    InterProiIPR005797. Cyt_b/b6_N.
    IPR023530. Cyt_B6_PetB.
    IPR027387. Cytb/b6-like.
    IPR016174. Di-haem_cyt_TM.
    [Graphical view]
    PfamiPF13631. Cytochrom_B_N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000032. Cytochrome_b6. 1 hit.
    SUPFAMiSSF81342. SSF81342. 1 hit.
    PROSITEiPS51002. CYTB_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P83791-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANVYDWFQE RLEIQALADD VTSKYVPPHV NIFYCLGGIT LTCFLIQFAT    50
    GFAMTFYYKP TVTEAYASVQ YIMNEVSFGW LIRSIHRWSA SMMVLMMILH 100
    VFRVYLTGGF KKPRELTWIS GVILAVITVS FGVTGYSLPW DQVGYWAVKI 150
    VSGVPEAIPV VGVLISDLLR GGSSVGQATL TRYYSAHTFV LPWLIAVFML 200
    LHFLMIRKQG ISGPL 215
    Length:215
    Mass (Da):24,227
    Last modified:March 15, 2004 - v1
    Checksum:i4FDCD9336CD31642
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VF5 X-ray 3.00 A/N 1-215 [» ]
    2D2C X-ray 3.80 A/N 1-215 [» ]
    2E74 X-ray 3.00 A 1-215 [» ]
    2E75 X-ray 3.55 A 1-215 [» ]
    2E76 X-ray 3.41 A 1-215 [» ]
    4H0L X-ray 3.25 A 1-215 [» ]
    4H13 X-ray 3.07 A 1-215 [» ]
    4I7Z X-ray 2.80 A 1-215 [» ]
    ProteinModelPortali P83791.
    SMRi P83791. Positions 1-215.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P83791.

    Family and domain databases

    Gene3Di 1.20.810.10. 1 hit.
    HAMAPi MF_00633. Cytb6_f_cytb6.
    InterProi IPR005797. Cyt_b/b6_N.
    IPR023530. Cyt_B6_PetB.
    IPR027387. Cytb/b6-like.
    IPR016174. Di-haem_cyt_TM.
    [Graphical view ]
    Pfami PF13631. Cytochrom_B_N_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000032. Cytochrome_b6. 1 hit.
    SUPFAMi SSF81342. SSF81342. 1 hit.
    PROSITEi PS51002. CYTB_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Full subunit coverage liquid chromatography electrospray ionization mass spectrometry (LCMS+) of an oligomeric membrane protein: cytochrome b(6)f complex from spinach and the cyanobacterium Mastigocladus laminosus."
      Whitelegge J.P., Zhang H., Aguilera R., Taylor R.M., Cramer W.A.
      Mol. Cell. Proteomics 1:816-827(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: HEME-BINDING.
    2. "Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity."
      Kurisu G., Zhang H., Smith J.L., Cramer W.A.
      Science 302:1009-1014(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), METAL-BINDING SITES.

    Entry informationi

    Entry nameiCYB6_MASLA
    AccessioniPrimary (citable) accession number: P83791
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: March 15, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Heme 1 (or BH or b566) is high-potential and absorbs at about 566 nm, and heme 2 (or BL or b562) is low-potential and absorbs at about 562 nm.UniRule annotation

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3