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P83791

- CYB6_MASLA

UniProt

P83791 - CYB6_MASLA

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Protein

Cytochrome b6

Gene

petB

Organism
Mastigocladus laminosus (Fischerella sp.)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Cofactori

hemeNote: Binds 2 heme groups. One heme group is bound covalently by a single cysteine link, the other one non-covalently.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351Heme 1 (covalent; via 1 link)
Metal bindingi86 – 861Iron (heme 2 axial ligand)
Metal bindingi100 – 1001Iron (heme 1 axial ligand)
Metal bindingi187 – 1871Iron (heme 2 axial ligand)
Metal bindingi202 – 2021Iron (heme 1 axial ligand)

GO - Molecular functioni

  1. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: UniProtKB-HAMAP
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. oxidoreductase activity Source: InterPro

GO - Biological processi

  1. photosynthesis Source: UniProtKB-HAMAP
  2. respiratory electron transport chain Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6UniRule annotation
Gene namesi
Name:petBUniRule annotation
OrganismiMastigocladus laminosus (Fischerella sp.)
Taxonomic identifieri83541 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaStigonematalesMastigocladus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei32 – 5221HelicalUniRule annotationAdd
BLAST
Transmembranei90 – 11021HelicalUniRule annotationAdd
BLAST
Transmembranei116 – 13621HelicalUniRule annotationAdd
BLAST
Transmembranei186 – 20621HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. thylakoid membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Cytochrome b6PRO_0000061827Add
BLAST

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.

Structurei

Secondary structure

1
215
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 129Combined sources
Helixi15 – 239Combined sources
Helixi32 – 354Combined sources
Helixi36 – 5419Combined sources
Turni55 – 573Combined sources
Turni62 – 643Combined sources
Helixi65 – 7410Combined sources
Helixi79 – 10527Combined sources
Turni106 – 1105Combined sources
Helixi112 – 1143Combined sources
Helixi115 – 13622Combined sources
Turni137 – 1393Combined sources
Helixi142 – 15110Combined sources
Helixi152 – 1576Combined sources
Turni159 – 1613Combined sources
Helixi162 – 1709Combined sources
Beta strandi172 – 1743Combined sources
Helixi177 – 18812Combined sources
Helixi190 – 20920Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VF5X-ray3.00A/N1-215[»]
2D2CX-ray3.80A/N1-215[»]
2E74X-ray3.00A1-215[»]
2E75X-ray3.55A1-215[»]
2E76X-ray3.41A1-215[»]
4H0LX-ray3.25A1-215[»]
4H13X-ray3.07A1-215[»]
4I7ZX-ray2.80A1-215[»]
4PV1X-ray3.00A1-215[»]
ProteinModelPortaliP83791.
SMRiP83791. Positions 1-215.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83791.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family. PetB subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.810.10. 1 hit.
HAMAPiMF_00633. Cytb6_f_cytb6.
InterProiIPR005797. Cyt_b/b6_N.
IPR023530. Cyt_B6_PetB.
IPR027387. Cytb/b6-like.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF13631. Cytochrom_B_N_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000032. Cytochrome_b6. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
PROSITEiPS51002. CYTB_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83791-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANVYDWFQE RLEIQALADD VTSKYVPPHV NIFYCLGGIT LTCFLIQFAT
60 70 80 90 100
GFAMTFYYKP TVTEAYASVQ YIMNEVSFGW LIRSIHRWSA SMMVLMMILH
110 120 130 140 150
VFRVYLTGGF KKPRELTWIS GVILAVITVS FGVTGYSLPW DQVGYWAVKI
160 170 180 190 200
VSGVPEAIPV VGVLISDLLR GGSSVGQATL TRYYSAHTFV LPWLIAVFML
210
LHFLMIRKQG ISGPL
Length:215
Mass (Da):24,227
Last modified:March 15, 2004 - v1
Checksum:i4FDCD9336CD31642
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VF5 X-ray 3.00 A/N 1-215 [» ]
2D2C X-ray 3.80 A/N 1-215 [» ]
2E74 X-ray 3.00 A 1-215 [» ]
2E75 X-ray 3.55 A 1-215 [» ]
2E76 X-ray 3.41 A 1-215 [» ]
4H0L X-ray 3.25 A 1-215 [» ]
4H13 X-ray 3.07 A 1-215 [» ]
4I7Z X-ray 2.80 A 1-215 [» ]
4PV1 X-ray 3.00 A 1-215 [» ]
ProteinModelPortali P83791.
SMRi P83791. Positions 1-215.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P83791.

Family and domain databases

Gene3Di 1.20.810.10. 1 hit.
HAMAPi MF_00633. Cytb6_f_cytb6.
InterProi IPR005797. Cyt_b/b6_N.
IPR023530. Cyt_B6_PetB.
IPR027387. Cytb/b6-like.
IPR016174. Di-haem_cyt_TM.
[Graphical view ]
Pfami PF13631. Cytochrom_B_N_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000032. Cytochrome_b6. 1 hit.
SUPFAMi SSF81342. SSF81342. 1 hit.
PROSITEi PS51002. CYTB_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Full subunit coverage liquid chromatography electrospray ionization mass spectrometry (LCMS+) of an oligomeric membrane protein: cytochrome b(6)f complex from spinach and the cyanobacterium Mastigocladus laminosus."
    Whitelegge J.P., Zhang H., Aguilera R., Taylor R.M., Cramer W.A.
    Mol. Cell. Proteomics 1:816-827(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: HEME-BINDING.
  2. "Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity."
    Kurisu G., Zhang H., Smith J.L., Cramer W.A.
    Science 302:1009-1014(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), METAL-BINDING SITES.

Entry informationi

Entry nameiCYB6_MASLA
AccessioniPrimary (citable) accession number: P83791
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 15, 2004
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Heme 1 (or BH or b566) is high-potential and absorbs at about 566 nm, and heme 2 (or BL or b562) is low-potential and absorbs at about 562 nm.UniRule annotation

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3