ID   P83787_MARNT            Unreviewed;       326 AA.
AC   P83787;
DT   15-MAR-2004, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2004, sequence version 1.
DT   27-MAR-2024, entry version 100.
DE   SubName: Full=Cytochrome c peroxidase {ECO:0000313|PDB:1NML};
OS   Marinobacter nauticus (Marinobacter hydrocarbonoclasticus) (Marinobacter
OS   aquaeolei).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Marinobacteraceae; Marinobacter.
OX   NCBI_TaxID=2743 {ECO:0000313|PDB:1NML};
RN   [1] {ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5}
RP   X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH CALCIUM AND HEME,
RP   AND ACTIVE SITE.
RX   PubMed=15274917; DOI=10.1016/j.str.2004.03.025;
RA   Dias J.M., Alves T., Bonifacio C., Pereira A.S., Trincao J., Bourgeois D.,
RA   Moura I., Romao M.J.;
RT   "Structural basis for the mechanism of Ca(2+) activation of the di-heme
RT   cytochrome c peroxidase from Pseudomonas nautica 617.";
RL   Structure 12:961-973(2004).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000256|PIRSR:PIRSR000294-1};
CC       Note=Binds 2 heme groups. {ECO:0000256|PIRSR:PIRSR000294-1};
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000256|ARBA:ARBA00004418}.
CC   -!- PTM: Binds 2 heme groups per subunit. {ECO:0000256|PIRSR:PIRSR000294-
CC       1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PDB; 1NML; X-ray; 2.20 A; A=1-326.
DR   PDB; 1RZ5; X-ray; 2.40 A; A=1-326.
DR   PDB; 1RZ6; X-ray; 2.20 A; A=1-326.
DR   PDBsum; 1NML; -.
DR   PDBsum; 1RZ5; -.
DR   PDBsum; 1RZ6; -.
DR   AlphaFoldDB; P83787; -.
DR   SMR; P83787; -.
DR   DrugBank; DB04272; Citric acid.
DR   PeroxiBase; 5023; MaqDiHCcP_617.
DR   EvolutionaryTrace; P83787; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.760.10; Cytochrome c-like domain; 2.
DR   InterPro; IPR009056; Cyt_c-like_dom.
DR   InterPro; IPR036909; Cyt_c-like_dom_sf.
DR   InterPro; IPR004852; Di-haem_cyt_c_peroxidsae.
DR   InterPro; IPR026259; MauG/Cytc_peroxidase.
DR   PANTHER; PTHR30600; CYTOCHROME C PEROXIDASE-RELATED; 1.
DR   PANTHER; PTHR30600:SF7; CYTOCHROME C PEROXIDASE-RELATED; 1.
DR   Pfam; PF03150; CCP_MauG; 1.
DR   Pfam; PF00034; Cytochrom_C; 1.
DR   PIRSF; PIRSF000294; Cytochrome-c_peroxidase; 1.
DR   SUPFAM; SSF46626; Cytochrome c; 2.
DR   PROSITE; PS51007; CYTC; 2.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5};
KW   Calcium {ECO:0007829|PDB:1RZ5};
KW   Heme {ECO:0000256|ARBA:ARBA00022617, ECO:0000256|PIRSR:PIRSR000294-1};
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR000294-2};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR000294-2};
KW   Periplasm {ECO:0000256|ARBA:ARBA00022764};
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          29..137
FT                   /note="Cytochrome c"
FT                   /evidence="ECO:0000259|PROSITE:PS51007"
FT   DOMAIN          183..300
FT                   /note="Cytochrome c"
FT                   /evidence="ECO:0000259|PROSITE:PS51007"
FT   ACT_SITE        114
FT                   /note="EMO_00062 proton shuttle (general acid/base)"
FT                   /evidence="ECO:0007829|PDB:1NML"
FT   BINDING         51
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_note="covalent"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ6"
FT   BINDING         51
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_note="covalent"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         51
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /note="covalent"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000294-1"
FT   BINDING         54
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_note="covalent"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ6"
FT   BINDING         54
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_note="covalent"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         54
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /note="covalent"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000294-1"
FT   BINDING         55
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /ligand_part_note="axial binding residue"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         55
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /ligand_part_note="axial binding residue"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ6"
FT   BINDING         55
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000294-2"
FT   BINDING         71
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /ligand_part_note="axial binding residue"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ6"
FT   BINDING         71
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000294-2"
FT   BINDING         78
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ6"
FT   BINDING         79
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         89
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         89
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:1NML"
FT   BINDING         93
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ6"
FT   BINDING         94
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         197
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /ligand_note="covalent"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5"
FT   BINDING         197
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /note="covalent"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000294-1"
FT   BINDING         200
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /ligand_note="covalent"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5"
FT   BINDING         200
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /note="covalent"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000294-1"
FT   BINDING         201
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /ligand_part_note="axial binding residue"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5"
FT   BINDING         201
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000294-2"
FT   BINDING         216
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5"
FT   BINDING         246
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         246
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ6"
FT   BINDING         256
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         258
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0007829|PDB:1RZ5"
FT   BINDING         260
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5"
FT   BINDING         261
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5"
FT   BINDING         275
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="3"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /ligand_part_note="axial binding residue"
FT                   /evidence="ECO:0007829|PDB:1NML, ECO:0007829|PDB:1RZ5"
FT   BINDING         275
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000294-2"
SQ   SEQUENCE   326 AA;  35355 MW;  FAAABCAC1E4652FF CRC64;
     DNLMERANSM FEPIPKYPPV IDGNELTQAK VELGKMEFFE PRLSSSHLIS CNTCHNVGLG
     GDDELPTSIG HGWQKGPRNS PTVFNAVFNA AQFWDGRAAD LAEQAKGPVQ AGVEMSSTPD
     RVVATLKSMP EYIERFEDAF PGQENPVTFD NMAVAIEAYE ATLITPEAPF DKYLRGDTSA
     LNESEKEGLA LFMDRGCTAC HSGVNLGGQN YYPFGLVAKP GAEILPEGDK GRFSVTETAS
     DEYVFRASPL RNIELTAPYF HSGAVWSLEE AVAVMGTAQL GTELNNDEVK SIVAFLKTLT
     GNVPEVTYPV LPPSTANTPK PVDMIP
//