Reviewed, UniProtKB/Swiss-Prot P83783 (SAHH_CANAL)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
      Short name=AdoHcyase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase

Gene names

Name:	SAH1
ORF Names:	CaO19.3911, CaO19.11392

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
Subcellular location	Cytoplasm Ref.2.
Miscellaneous	Has antigenic properties. Elicits a specific immune response in systemic candidiasis human patients undergoing malignant hematological disorders.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Adenosylhomocysteinase		PRO_0000116934
Regions
	Region	187 – 354	168	NAD binding By similarity
Sites
	Binding site	59	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P83783-1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 85A6D18CE85517E3	FASTA	450	49,072
	10 20 30 40 50 60 MSSAPATNYK VADISLAAFG RKDIELSENE MPGLMYIRKK YGPSQPLKGA RIAGCLHMTI 70 80 90 100 110 120 QTAVLIETLV ALGAEVTWSS CNIFSTQDHA AAAIAAAGVP VFAWKGETEE EYQWCIEQQL 130 140 150 160 170 180 FAFKDGKKLN LILDDGGDLT SLVHEKYPEM LEDCYGLSEE TTTGVHHLYK SLRDGKLKVP 190 200 210 220 230 240 AINVNDSVTK SKFDNLYGCR ESLVDGIKRA TDVMIAGKVA IVAGFGDVGK GCAMALHGMG 250 260 270 280 290 300 ARVIVTEIDP INALQAAVSG YQVAPMDEVA SIGQIFVTTT GCRDIITGKH FEQMPEDAIV 310 320 330 340 350 360 CNIGHFDIEI DVAWLKANAE SVVNIKPQVD RYLMKNGRHV ILLADGRLVN LGCATGHSSF 370 380 390 400 410 420 VMSCSFSNQV LAQIALFNAD NKEFREKFPE FAKTGPFDVG VHLLPKVLDE TVARCHLDHL 430 440 450 GAKLTTLTET QAEYLGIPEE GPYKADIYRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.
[2]	"Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies." Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C. Proteomics 4:3084-3106(2004) [PubMed: 15378761] [Abstract] Cited for: PROTEIN SEQUENCE OF 179-190 AND 193-200, SUBCELLULAR LOCATION, ANTIGENICITY. Strain: SC5314. Tissue: Protoplast.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000012 Genomic DNA. Translation: EAL03041.1. AACQ01000011 Genomic DNA. Translation: EAL03204.1.
RefSeq	XP_721821.1. XP_721980.1.
3D structure databases
SMR	P83783. Positions 9-450.
ModBase	Search...
Genome annotation databases
GeneID	3636277. 3636452.
KEGG	cal:CaO19.11392. cal:CaO19.3911.
Organism-specific databases
CGD	CAL0002855. SAH1.
Phylogenomic databases
OMA	VITHDHM.
OrthoDB	EOG9M937G.
PhylomeDB	P83783.
Enzyme and pathway databases
BRENDA	3.3.1.1. 1124.
Family and domain databases
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_CANAL

Accession

Primary (citable) accession number: P83783
Secondary accession number(s): Q5AKA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	May 5, 2009
Last modified:	February 9, 2010
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)