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Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Binding sitei86 – 861SubstratePROSITE-ProRule annotation
Binding sitei92 – 921SubstratePROSITE-ProRule annotation
Binding sitei99 – 991NADBy similarity
Binding sitei124 – 1241SubstratePROSITE-ProRule annotation
Binding sitei158 – 1581SubstratePROSITE-ProRule annotation
Active sitei184 – 1841Proton acceptorBy similarity
Binding sitei238 – 2381NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 147NADBy similarity
Nucleotide bindingi122 – 1243NADBy similarity

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: UniProtKB-EC
  • malate dehydrogenase activity Source: CGD

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • malate metabolic process Source: InterPro
  • tricarboxylic acid cycle Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Gene namesi
Name:MDH1
Synonyms:MDH2
ORF Names:CaO19.7481
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0005697. MDH1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Malate dehydrogenase, cytoplasmicPRO_0000113337Add
BLAST

2D gel databases

COMPLUYEAST-2DPAGEP83778.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi5476.CAL0005697.

Structurei

3D structure databases

ProteinModelPortaliP83778.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
InParanoidiP83778.
KOiK00026.
OrthoDBiEOG7GJ6Q7.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83778-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVAILGAA GGIGQPLSLL TKLNPNVDEL ALFDVVNVPG VGADLSHINS
60 70 80 90 100
DSKTQSYLPK DKEDKTALAA ALKGSDLVII PAGVPRKPGM TRDDLFNINA
110 120 130 140 150
SIVQGLAEGI AANSPKAFVL VISNPVNSTV PIVAETLQAK GVYDPARLFG
160 170 180 190 200
VTTLDIVRAN TFISQLFLDQ TKPSDFNINV VGGHSGETIV PLYSLGNSKQ
210 220 230 240 250
YYDILSEEQK KELIKRVQFG GDEVVQAKNG AGSATLSMAY AGYRLAESIL
260 270 280 290 300
AAVNGKTDIV ECTFLNLDSS IKGASEARKL VKDLDFFSLP VQLGKNGITE
310 320 330
VKYDILNQIS DDEKKLLEVA IEQLQKNIEK GVSFAKK
Length:337
Mass (Da):36,004
Last modified:October 19, 2011 - v2
Checksum:i0BB466CAA0010CE4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 292DE → TD AA sequence (PubMed:15378761).Curated
Sequence conflicti33 – 364FDVV → YDIR AA sequence (PubMed:15378761).Curated
Sequence conflicti42 – 421G → A AA sequence (PubMed:15378761).Curated
Sequence conflicti45 – 451L → V AA sequence (PubMed:15378761).Curated
Sequence conflicti48 – 558INSDSKTQ → VPTNSTVK AA sequence (PubMed:15378761).Curated
Sequence conflicti98 – 981I → T AA sequence (PubMed:15378761).Curated
Sequence conflicti104 – 1041Q → R AA sequence (PubMed:15378761).Curated
Sequence conflicti240 – 2401Y → Q AA sequence (PubMed:15378761).Curated
Sequence conflicti243 – 2431Y → A AA sequence (PubMed:15378761).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000039 Genomic DNA. Translation: EAK99723.1.
RefSeqiXP_718638.1. XM_713545.1.

Genome annotation databases

GeneIDi3639667.
KEGGical:CaO19.7481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000039 Genomic DNA. Translation: EAK99723.1.
RefSeqiXP_718638.1. XM_713545.1.

3D structure databases

ProteinModelPortaliP83778.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5476.CAL0005697.

2D gel databases

COMPLUYEAST-2DPAGEP83778.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3639667.
KEGGical:CaO19.7481.

Organism-specific databases

CGDiCAL0005697. MDH1.

Phylogenomic databases

eggNOGiCOG0039.
InParanoidiP83778.
KOiK00026.
OrthoDBiEOG7GJ6Q7.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. "Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies."
    Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C.
    Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-36; 39-55; 95-104 AND 231-244, SUBCELLULAR LOCATION, ANTIGENICITY.
    Strain: SC5314 / ATCC MYA-2876.
    Tissue: Protoplast.

Entry informationi

Entry nameiMDHC_CANAL
AccessioniPrimary (citable) accession number: P83778
Secondary accession number(s): Q5AAK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 19, 2011
Last modified: May 27, 2015
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Has antigenic properties. Elicits a specific immune response in systemic candidiasis human patients undergoing malignant hematological disorders.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.