Reviewed, UniProtKB/Swiss-Prot P83777 (IPYR_CANAL)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	IPP1
ORF Names:	CaO19.3590, CaO19.11072

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm Ref.2.
Miscellaneous	Has antigenic properties. Elicits a specific immune response in systemic candidiasis human patients undergoing malignant hematological disorders.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Inorganic pyrophosphatase		PRO_0000137580
Sites
	Metal binding	117	1	Magnesium 1 By similarity
	Metal binding	122	1	Magnesium 1 By similarity
	Metal binding	122	1	Magnesium 2 By similarity
	Metal binding	154	1	Magnesium 1 By similarity
	Binding site	80	1	Inorganic pyrophosphate By similarity
Natural variations
	Natural variant	27	1	P → L in allele CaO19.3590.

Sequences

	Sequence		Length	Mass (Da)
	P83777-1 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: 3EF4D51446F19083	FASTA	288	32,128
	10 20 30 40 50 60 MSSYSTRQVG AANTLDYKVY IEKDGKPVSP FHDIPLYANE EKTILNMIVE VPRWTNAKLE 70 80 90 100 110 120 ISKEQKLNPI IQDTKKGKLR FVRNCFPHHG YIHNYGAFPQ TWEDPNQSHP ETKAKGDNDP 130 140 150 160 170 180 LDVCEIGEKV ATVGEVKQVK VLGVMALLDE GETDWKVIVI DVNDPLAPKL NDIEDVETHL 190 200 210 220 230 240 PGLLRATNEW FRIYKIPDGK PENQFAFSGE CKNKKYAEEV IGECAEAWEK LIKGESVDSK 250 260 270 280 GIDLTNTTLS STPSYSDAAA QEIPSASPAP AAPIDKSIDK WFFISGAH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.
[2]	"Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies." Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C. Proteomics 4:3084-3106(2004) [PubMed: 15378761] [Abstract] Cited for: PROTEIN SEQUENCE OF 186-192, SUBCELLULAR LOCATION, ANTIGENICITY. Strain: SC5314. Tissue: Protoplast.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000102 Genomic DNA. Translation: EAK95424.1. AACQ01000103 Genomic DNA. Translation: EAK95370.1.
RefSeq	XP_714437.1. XP_714485.1.
3D structure databases
SMR	P83777. Positions 3-286.
ModBase	Search...
Genome annotation databases
GeneID	3643838. 3643874.
KEGG	cal:CaO19.11072. cal:CaO19.3590.
Organism-specific databases
CGD	CAL0006016. IPP1.
Phylogenomic databases
OMA	SESPFCC.
OrthoDB	EOG93JDNG.
PhylomeDB	P83777.
Enzyme and pathway databases
BRENDA	3.6.1.1. 1124.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_CANAL

Accession

Primary (citable) accession number: P83777
Secondary accession number(s): Q59Y59, Q59YB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 24, 2006
Last modified:	January 19, 2010
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)