P83776 (HXKB_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hexokinase-2
EC=2.7.1.1

Alternative name(s):
Cytoplasmic antigenic protein 3
Hexokinase PII
Hexokinase-B

Gene names

Name:	HXK2
ORF Names:	CaO19.542, CaO19.8176

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Main glucose phosphorylating enzyme. May play a regulatory role in both induction and repression of gene expression by glucose By similarity.
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Ref.2.
Miscellaneous	Has antigenic properties. Elicits a specific immune response in systemic candidiasis human patients undergoing malignant hematological disorders.
Sequence similarities	Belongs to the hexokinase family.
Sequence caution	The sequence EAK98532.1 differs from that shown. Reason: Frameshift at positions 62 and 67.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Hexokinase-2		PRO_0000089303
Natural variations
	Natural variant	64 – 65	2	PM → LK in allele CaO19.8176.
	Natural variant	72	1	D → I in allele CaO19.8176.
	Natural variant	100	1	G → A in allele CaO19.8176.
	Natural variant	383	1	C → R in allele CaO19.8176.
	Natural variant	410	1	K → E in allele CaO19.8176.

Sequences

	Sequence		Length	Mass (Da)
	P83776 [UniParc]. Last modified October 19, 2011. Version 2. Checksum: 7248469B1536C23A	FASTA	484	53,416
	10 20 30 40 50 60 MVHLGPKPAQ KRKGTFTDVS PQLLEALKPI QEQFTISADK LRAIVKHFIS ELDRGLSKAG 70 80 90 100 110 120 GNIPMIPGWV MDFPTGKETG SYLAIDLGGT NLRVVLVKLG GNRDFDTTQS KFALPAHMRT 130 140 150 160 170 180 ATSDELWDFI AKCLKEFVDE IYPDGCSEPL PLGFTFSYPA SQNRINEGIL QRWTKGWSID 190 200 210 220 230 240 GIEGKDVVPM LQKAIKKVGV PIDVVALIND TTGTLVASMY TDPEAKMGLI FGTGVNGAYF 250 260 270 280 290 300 DVVKDIPKLE GKCPSDIPPE SPMAINCEYG SFDNEKYILP RTKYDVQIDE ESPRPGQQTF 310 320 330 340 350 360 EKMISGYYLG EVLRLILLEF AEEKKLIFKG QNLDKLKVPY VMDASYPSKI EEDPFENLSD 370 380 390 400 410 420 VADLFREKLG IETTEPERKI IRCLAELIGE RSARFSVCGI AAICQKRGYK TAHCAADGSV 430 440 450 460 470 480 YNKYPGFKER TAQALRDIYE WPADVKDPII IVPAEDGSGV GAAVIAALTE KRLKEGKSVG LLGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies." Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C. Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 176-185 AND 317-325, SUBCELLULAR LOCATION, ANTIGENICITY. Strain: SC5314 / ATCC MYA-2876. Tissue: Protoplast.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000054 Genomic DNA. Translation: EAK98532.1. Frameshift. AACQ01000055 Genomic DNA. Translation: EAK98436.1.
RefSeq	XP_717405.1. XM_712312.1. XP_717498.1. XM_712405.1.
3D structure databases
ProteinModelPortal	P83776.
SMR	P83776. Positions 18-484.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0000198.
2D gel databases
COMPLUYEAST-2DPAGE	P83776.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3640936. 3641015.
KEGG	cal:CaO19.542. cal:CaO19.8176.
Organism-specific databases
CGD	CAL0000198. HXK2. CAL0078705. HXK2.
Phylogenomic databases
eggNOG	COG5026.
KO	K00844.
Enzyme and pathway databases
UniPathway	UPA00242.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR022673. Hexokinase_C. IPR022672. Hexokinase_N. [Graphical view]
PANTHER	PTHR19443. PTHR19443. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProtoNet	Search...

Entry information

Entry name

HXKB_CANAL

Accession

Primary (citable) accession number: P83776
Secondary accession number(s): Q5A6L2, Q5A6V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program