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P83763 (CBNB_CUPNE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloromuconate cycloisomerase CbnB

EC=5.5.1.7
Alternative name(s):
Muconate cycloisomerase II CbnB
Gene names
Name:cbnB
Encoded onPlasmid pENH91
OrganismCupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha)
Taxonomic identifier106590 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactor

Manganese By similarity.

Pathway

Aromatic compound metabolism; 3-chlorocatechol degradation.

Sequence similarities

Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Chloromuconate cycloisomerase CbnB
PRO_0000171255

Sites

Active site1651Proton acceptor By similarity
Active site3231Proton donor By similarity
Metal binding1941Manganese By similarity
Metal binding2201Manganese By similarity
Metal binding2451Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
P83763 [UniParc].

Last modified March 1, 2004. Version 1.
Checksum: 195D3101CA18B31C

FASTA37039,465
        10         20         30         40         50         60 
MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVTAGG LVGIGEGGSV GGPTWGSESA 

        70         80         90        100        110        120 
ETIKVIIDNY LAPLLIGKDA SNLSEARALM DRAVTGNLSA KAAIDIALHD LKARALNLSI 

       130        140        150        160        170        180 
ADLIGGTMRK SIPIAWTLAS GDTARDIDSA LEMIEARRHN RFKVKLGART PAQDLEHIRS 

       190        200        210        220        230        240 
IVKAVGDKAS VRVDVNQGWD EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV 

       250        260        270        280        290        300 
AILADESLSS LSSAFELARD RAVDAFSLKL CNMGGIANTL KVAAIAEAAG ISSYGGTMLD 

       310        320        330        340        350        360 
STVGTAAALH VYATLPSLPY GCELIGPWVL SDRLTQQDLE IKDFEVHLPV GSGLGVDLDH 

       370 
DKVRHYTRAA 

« Hide

References

[1]"The chlorocatechol-catabolic transposon Tn5707 of Alcaligenes eutrophus NH9, carrying a gene cluster highly homologous to that in the 1,2,4-trichlorobenzene-degrading bacterium Pseudomonas sp. strain P51, confers the ability to grow on 3-chlorobenzoate."
Ogawa N., Miyashita K.
Appl. Environ. Microbiol. 65:724-731(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NH9.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB019032 Genomic DNA. Translation: BAA74531.1.

3D structure databases

ProteinModelPortalP83763.
SMRP83763. Positions 1-369.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00083.

Family and domain databases

Gene3D3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]
PANTHERPTHR13794. PTHR13794. 1 hit.
PfamPF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsTIGR02534. mucon_cyclo. 1 hit.
PROSITEPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCBNB_CUPNE
AccessionPrimary (citable) accession number: P83763
Secondary accession number(s): Q93T14, Q9WXC9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2004
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways