P83763 (CBNB_CUPNE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloromuconate cycloisomerase CbnB
EC=5.5.1.7

Alternative name(s):
Muconate cycloisomerase II CbnB

Gene names

Name:

cbnB

Encoded on

Plasmid pENH91

Organism

Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha)

Taxonomic identifier

106590 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.
Cofactor	Manganese By similarity.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Sequence similarities	Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Manganese Metal-binding
Molecular function	Isomerase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW cellular amino acid catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	chloromuconate cycloisomerase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: InterPro muconate cycloisomerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Chloromuconate cycloisomerase CbnB		PRO_0000171255
Sites
	Active site	165	1	Proton acceptor By similarity
	Active site	323	1	Proton donor By similarity
	Metal binding	194	1	Manganese By similarity
	Metal binding	220	1	Manganese By similarity
	Metal binding	245	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P83763 [UniParc]. Last modified March 1, 2004. Version 1. Checksum: 195D3101CA18B31C	FASTA	370	39,465
	10 20 30 40 50 60 MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVTAGG LVGIGEGGSV GGPTWGSESA 70 80 90 100 110 120 ETIKVIIDNY LAPLLIGKDA SNLSEARALM DRAVTGNLSA KAAIDIALHD LKARALNLSI 130 140 150 160 170 180 ADLIGGTMRK SIPIAWTLAS GDTARDIDSA LEMIEARRHN RFKVKLGART PAQDLEHIRS 190 200 210 220 230 240 IVKAVGDKAS VRVDVNQGWD EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV 250 260 270 280 290 300 AILADESLSS LSSAFELARD RAVDAFSLKL CNMGGIANTL KVAAIAEAAG ISSYGGTMLD 310 320 330 340 350 360 STVGTAAALH VYATLPSLPY GCELIGPWVL SDRLTQQDLE IKDFEVHLPV GSGLGVDLDH 370 DKVRHYTRAA

References

[1]

"The chlorocatechol-catabolic transposon Tn5707 of Alcaligenes eutrophus NH9, carrying a gene cluster highly homologous to that in the 1,2,4-trichlorobenzene-degrading bacterium Pseudomonas sp. strain P51, confers the ability to grow on 3-chlorobenzoate." Ogawa N., Miyashita K. Appl. Environ. Microbiol. 65:724-731(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NH9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB019032 Genomic DNA. Translation: BAA74531.1.
3D structure databases
ProteinModelPortal	P83763.
SMR	P83763. Positions 1-369.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00083.
Family and domain databases
InterPro	IPR013370. Chloromuconate_cycloisomerase. IPR018110. Mandel_Rmase/mucon_lact_enz_CS. IPR013342. Mandelate_racemase_C. IPR013341. Mandelate_racemase_N. IPR001354. MR_MLE. [Graphical view]
PANTHER	PTHR13794. PTHR13794. 1 hit. PTHR13794:SF12. PTHR13794:SF12. 1 hit.
Pfam	PF02746. MR_MLE_N. 1 hit. [Graphical view]
SMART	SM00922. MR_MLE. 1 hit. [Graphical view]
TIGRFAMs	TIGR02534. mucon_cyclo. 1 hit.
PROSITE	PS00908. MR_MLE_1. 1 hit. PS00909. MR_MLE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBNB_CUPNE

Accession

Primary (citable) accession number: P83763
Secondary accession number(s): Q93T14, Q9WXC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	March 1, 2004
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program