Photosystem Q(B) protein precursor - Arabidopsis thaliana (Mouse-ear cress)

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P83755 (PSBA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Photosystem Q(B) protein
EC=1.10.3.9

Alternative name(s):
32 kDa thylakoid membrane protein
Photosystem II protein D1

Gene names

Name:	psbA
Ordered Locus Names:	AtCg00020

Encoded on

Plastid; Chloroplast

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is one of the two reaction center proteins of photosystem II. HAMAP-Rule MF_01379
Catalytic activity	2 H₂O + 2 plastoquinone + 4 light = O₂ + 2 plastoquinol. HAMAP-Rule MF_01379
Cofactor	The psbA/B heterodimer binds P680, the primary electron donor of PSII. It shares a non-heme iron and each subunit binds additional chlorophylls and pheophytin. PsbA provides most of the ligands for the Mn-cluster of the oxygen-evolving complex By similarity.
Subunit structure	The psbA/B heterodimer binds the P680 chlorophylls and subsequent electron acceptors. PSII consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. PSII forms dimeric complexes By similarity. Interacts with PAM68. The nascent chain still attached to the ribosome interacts with FFC/ cpSRP54, but not with CAO/cpSRP43 or SecA. Ref.4 Ref.5
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein HAMAP-Rule MF_01379.
Post-translational modification	Phosphorylation occurs in normal plant growth light conditions. Rapid dephosphorylation occurs during heat shock. HAMAP-Rule MF_01379
Miscellaneous	Herbicides such as atrazine, BNT, diuron or ioxynil bind to Q(B) and block electron transport By similarity. HAMAP-Rule MF_01379
Sequence similarities	Belongs to the reaction center PufL/M/PsbA/D family.
Mass spectrometry	Molecular mass is 823.4 Da from positions 2 - 7. Determined by ESI. Ref.3

Ontologies

Keywords
Biological process	Electron transport Herbicide resistance Photosynthesis Transport
Cellular component	Chloroplast Membrane Photosystem II Plastid Thylakoid
Domain	Transmembrane Transmembrane helix
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	photosynthetic electron transport in photosystem II Inferred from electronic annotation. Source: InterPro response to herbicide Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW light-harvesting complex Inferred from electronic annotation. Source: InterPro photosystem II Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CAM4	P25854	2	EBI-1236013,EBI-1235664
CAM7	P59220	2	EBI-1236013,EBI-1236031
CML9	Q9S744	2	EBI-1236013,EBI-1236048

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.3

Chain

2 – 344

343

Photosystem Q(B) protein HAMAP-Rule MF_01379

PRO_0000090425

Propeptide

345 – 353

Potential

PRO_0000316439

Regions

Transmembrane

36 – 56

Helical; Potential

Transmembrane

109 – 129

Helical; Potential

Transmembrane

141 – 164

Helical; Potential

Transmembrane

192 – 218

Helical; Potential

Transmembrane

269 – 289

Helical; Potential

Sites

Metal binding

215

Iron; shared with heterodimeric partner By similarity

Metal binding

272

Iron; shared with heterodimeric partner By similarity

Site

344 – 345

Cleavage; by ctpA By similarity

Amino acid modifications

Modified residue

N-acetylthreonine Ref.3

Modified residue

Phosphothreonine Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P83755 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 79D3F384DAB4D610
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FASTA

353

38,937

        10         20         30         40         50         60 
MTAILERRES ESLWGRFCNW ITSTENRLYI GWFGVLMIPT LLTATSVFII AFIAAPPVDI 

        70         80         90        100        110        120 
DGIREPVSGS LLYGNNIISG AIIPTSAAIG LHFYPIWEAA SVDEWLYNGG PYELIVLHFL 

       130        140        150        160        170        180 
LGVACYMGRE WELSFRLGMR PWIAVAYSAP VAAATAVFLI YPIGQGSFSD GMPLGISGTF 

       190        200        210        220        230        240 
NFMIVFQAEH NILMHPFHML GVAGVFGGSL FSAMHGSLVT SSLIRETTEN ESANEGYRFG 

       250        260        270        280        290        300 
QEEETYNIVA AHGYFGRLIF QYASFNNSRS LHFFLAAWPV VGIWFTALGI STMAFNLNGF 

       310        320        330        340        350 
NFNQSVVDSQ GRVINTWADI INRANLGMEV MHERNAHNFP LDLAAVEAPS TNG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of the Arabidopsis thaliana chloroplast DNA region containing the genes psbA, trnH and rps19'." Liere K., Kestermann M., Mueller U., Link G. Curr. Genet. 28:128-130(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[2]	"Complete structure of the chloroplast genome of Arabidopsis thaliana." Sato S., Nakamura Y., Kaneko T., Asamizu E., Tabata S. DNA Res. 6:283-290(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Mass spectrometric resolution of reversible protein phosphorylation in photosynthetic membranes of Arabidopsis thaliana." Vener A.V., Harms A., Sussman M.R., Vierstra R.D. J. Biol. Chem. 276:6959-6966(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-7, PHOSPHORYLATION AT THR-2, ACETYLATION AT THR-2, MASS SPECTROMETRY. Strain: cv. Columbia.
[4]	"Interactions of ribosome nascent chain complexes of the chloroplast-encoded D1 thylakoid membrane protein with cpSRP54." Nilsson R., Brunner J., Hoffman N.E., van Wijk K.J. EMBO J. 18:733-742(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FFC/CPSRP54.
[5]	"The Arabidopsis thylakoid protein PAM68 is required for efficient D1 biogenesis and photosystem II assembly." Armbruster U., Zuhlke J., Rengstl B., Kreller R., Makarenko E., Ruhle T., Schunemann D., Jahns P., Weisshaar B., Nickelsen J., Leister D. Plant Cell 22:3439-3460(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PAM68.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79898 Genomic DNA. Translation: CAA56270.1. AP000423 Genomic DNA. Translation: BAA84365.1.
IPI	IPI00546934.
PIR	S57265.
RefSeq	NP_051039.1. NC_000932.1.
3D structure databases
ProteinModelPortal	P83755.
SMR	P83755. Positions 10-344.
ModBase	Search...
Protein-protein interaction databases
IntAct	P83755. 10 interactions.
STRING	3702.ATCG00020.1-P.
Proteomic databases
PaxDb	P83755.
PRIDE	P83755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	ATCG00020.1; ATCG00020.1; ATCG00020.
GeneID	844802.
KEGG	ath:ArthCp002.
Organism-specific databases
TAIR	AtCg00020.
Phylogenomic databases
eggNOG	NOG04871.
HOGENOM	HOG000246913.
KO	K02703.
OMA	CFTIAFI.
ProtClustDB	CHL00003.
Enzyme and pathway databases
BioCyc	MetaCyc:ATCG00020-MONOMER.
Gene expression databases
Genevestigator	P83755.
GermOnline	ATCG00020. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.85.10. 1 hit.
HAMAP	MF_01379. PSII_PsbA_D1.
InterPro	IPR000484. Photo_RC_L/M. IPR005867. PSII_D1. [Graphical view]
Pfam	PF00124. Photo_RC. 1 hit. [Graphical view]
PRINTS	PR00256. REACTNCENTRE.
SUPFAM	SSF81483. Photo_RC_L/M. 1 hit.
TIGRFAMs	TIGR01151. psbA. 1 hit.
PROSITE	PS00244. REACTION_CENTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSBA_ARATH

Accession

Primary (citable) accession number: P83755
Secondary accession number(s): Q33592

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents