P83748 (PRS30_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine protease 30
EC=3.4.21.-

Alternative name(s):
Distal intestinal serine protease
Transmembrane serine protease 1
Short name=TMSP-1
Transmembrane serine protease 8

Gene names

Name:	Prss30
Synonyms:	Disp, Tmprss8, Tmsp-1, Tmsp1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Selectively cleaves synthetic peptide substrates of trypsin. Activates the epithelial sodium channel ENaC. Ref.1
Enzyme regulation	Inhibited by aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor. Partially inhibited by PMSF and DFP. Ref.1
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Probable.
Tissue specificity	Expressed predominantly in kidney, small intestine and stomach and moderately in thymus, lung, spleen, testis and skin. In the kidney, expressed mainly in collecting duct of renal medulla and cortex. Ref.1
Induction	By aldosterone. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
Biophysicochemical properties	pH dependence: Optimum pH is 9.0.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Sodium
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from direct assay Ref.1. Source: UniProtKB sodium ion transport Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	anchored to plasma membrane Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	serine-type endopeptidase activity Inferred from direct assay Ref.1. Source: UniProtKB sodium channel regulator activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 30	9	Activation peptide		PRO_0000027864
	Chain	31 – 284	254	Serine protease 30 Ref.1		PRO_0000027865
	Propeptide	285 – 304	20	Removed in mature form Potential		PRO_0000027866
Regions
	Domain	31 – 271	241	Peptidase S1
Sites
	Active site	72	1	Charge relay system By similarity UniProtKB P49863
	Active site	122	1	Charge relay system By similarity UniProtKB P49863
	Active site	223	1	Charge relay system By similarity UniProtKB P49863
Amino acid modifications
	Lipidation	284	1	GPI-anchor amidated alanine Potential
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	232	1	N-linked (GlcNAc...) Potential
	Glycosylation	273	1	N-linked (GlcNAc...) Potential
	Disulfide bond	57 ↔ 73		By similarity UniProtKB P49863
	Disulfide bond	155 ↔ 229		By similarity UniProtKB P49863
	Disulfide bond	185 ↔ 208		By similarity UniProtKB P49863
	Disulfide bond	219 ↔ 247		By similarity UniProtKB P49863

Sequences

	Sequence		Length	Mass (Da)
	P83748 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: 825187B7DD70D789	FASTA	304	33,086
	10 20 30 40 50 60 MESWARCIFL LLLQILTGGR GDILHSGAGK IVGGQDAPEG RWPWQVSLRT EKEGHICGGS 70 80 90 100 110 120 LIHEVWVLTA AHCFRRPLNS SFYHVKVGGL TLSLTEPHST LVAVRNIFVY PTYLWEDASS 130 140 150 160 170 180 GDIALLRLDT PLQPSQFSPV CLPQAQAPLT PGTVCWVTGW GATHERELAS VLQELAVPLL 190 200 210 220 230 240 DSEDCERMYH IGETSLSGKR VIQSDMLCAG FVEGQKDSCQ GDSGGPLVCA INSSWIQVGI 250 260 270 280 290 300 TSWGIGCARP NKPGVYTRVP DYVDWIQRTL AENHSDAYGC RSRASGAYPA LLLVLLAFAL PESL

References

[1]

"Cloning and characterization of a transmembrane-type serine protease from rat kidney, a new sodium channel activator." Okumura Y., Nishikawa M., Cui P., Shiota M., Nakamura Y., Adachi M., Kitamura K., Tomita K., Kido H. Biol. Chem. 384:1483-1495(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION. Strain: Sprague-Dawley. Tissue: Kidney.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB073023 mRNA. Translation: BAD01655.1.
IPI	IPI00369252.
RefSeq	NP_955403.1. NM_199371.1.
UniGene	Rn.102783.
3D structure databases
ProteinModelPortal	P83748.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000037368.
Protein family/group databases
MEROPS	S01.042.
Proteomic databases
PRIDE	P83748.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	287106.
KEGG	rno:287106.
UCSC	RGD:735142. rat.
Organism-specific databases
CTD	30943.
RGD	735142. Prss30.
Phylogenomic databases
eggNOG	COG5640.
HOVERGEN	HBG013304.
InParanoid	P83748.
KO	K09639.
OrthoDB	EOG49CQ8F.
Gene expression databases
ArrayExpress	P83748.
Genevestigator	P83748.
GermOnline	ENSRNOG00000033266. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	625497.

Entry information

Entry name

PRS30_RAT

Accession

Primary (citable) accession number: P83748

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	February 16, 2004
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program