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P83748

- PRS30_RAT

UniProt

P83748 - PRS30_RAT

Protein

Serine protease 30

Gene

Prss30

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (16 Feb 2004)
      Previous versions | rss
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    Functioni

    Selectively cleaves synthetic peptide substrates of trypsin. Activates the epithelial sodium channel ENaC.1 Publication

    Enzyme regulationi

    Inhibited by aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor. Partially inhibited by PMSF and DFP.1 Publication

    pH dependencei

    Optimum pH is 9.0.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei72 – 721Charge relay systemBy similarity
    Active sitei122 – 1221Charge relay systemBy similarity
    Active sitei223 – 2231Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: UniProtKB
    2. sodium channel regulator activity Source: UniProtKB

    GO - Biological processi

    1. proteolysis Source: UniProtKB
    2. sodium ion transport Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Protein family/group databases

    MEROPSiS01.042.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine protease 30 (EC:3.4.21.-)
    Alternative name(s):
    Distal intestinal serine protease
    Transmembrane serine protease 1
    Short name:
    TMSP-1
    Transmembrane serine protease 8
    Gene namesi
    Name:Prss30
    Synonyms:DispBy similarity, Tmprss8, Tmsp-1, Tmsp11 Publication
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi735142. Prss30.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Propeptidei22 – 309Activation peptide1 PublicationSequence AnalysisPRO_0000027864
    Chaini31 – 284254Serine protease 30PRO_0000027865Add
    BLAST
    Propeptidei285 – 30420Removed in mature formCuratedPRO_0000027866Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi57 ↔ 73By similarityPROSITE-ProRule annotation
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi155 ↔ 229By similarityPROSITE-ProRule annotation
    Disulfide bondi185 ↔ 208By similarityPROSITE-ProRule annotation
    Disulfide bondi219 ↔ 247By similarityPROSITE-ProRule annotation
    Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence Analysis
    Lipidationi284 – 2841GPI-anchor amidated alanineCurated

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

    Proteomic databases

    PRIDEiP83748.

    Expressioni

    Tissue specificityi

    Expressed predominantly in kidney, small intestine and stomach and moderately in thymus, lung, spleen, testis and skin. In the kidney, expressed mainly in collecting duct of renal medulla and cortex.1 Publication

    Inductioni

    By aldosterone.1 Publication

    Gene expression databases

    GenevestigatoriP83748.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000037368.

    Structurei

    3D structure databases

    ProteinModelPortaliP83748.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 271241Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOVERGENiHBG013304.
    InParanoidiP83748.
    KOiK09639.
    PhylomeDBiP83748.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P83748-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESWARCIFL LLLQILTGGR GDILHSGAGK IVGGQDAPEG RWPWQVSLRT    50
    EKEGHICGGS LIHEVWVLTA AHCFRRPLNS SFYHVKVGGL TLSLTEPHST 100
    LVAVRNIFVY PTYLWEDASS GDIALLRLDT PLQPSQFSPV CLPQAQAPLT 150
    PGTVCWVTGW GATHERELAS VLQELAVPLL DSEDCERMYH IGETSLSGKR 200
    VIQSDMLCAG FVEGQKDSCQ GDSGGPLVCA INSSWIQVGI TSWGIGCARP 250
    NKPGVYTRVP DYVDWIQRTL AENHSDAYGC RSRASGAYPA LLLVLLAFAL 300
    PESL 304
    Length:304
    Mass (Da):33,086
    Last modified:February 16, 2004 - v1
    Checksum:i825187B7DD70D789
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073023 mRNA. Translation: BAD01655.1.
    RefSeqiNP_955403.1. NM_199371.1.
    UniGeneiRn.102783.

    Genome annotation databases

    GeneIDi287106.
    KEGGirno:287106.
    UCSCiRGD:735142. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073023 mRNA. Translation: BAD01655.1 .
    RefSeqi NP_955403.1. NM_199371.1.
    UniGenei Rn.102783.

    3D structure databases

    ProteinModelPortali P83748.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000037368.

    Protein family/group databases

    MEROPSi S01.042.

    Proteomic databases

    PRIDEi P83748.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 287106.
    KEGGi rno:287106.
    UCSCi RGD:735142. rat.

    Organism-specific databases

    CTDi 30943.
    RGDi 735142. Prss30.

    Phylogenomic databases

    eggNOGi COG5640.
    HOVERGENi HBG013304.
    InParanoidi P83748.
    KOi K09639.
    PhylomeDBi P83748.

    Miscellaneous databases

    NextBioi 625497.

    Gene expression databases

    Genevestigatori P83748.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a transmembrane-type serine protease from rat kidney, a new sodium channel activator."
      Okumura Y., Nishikawa M., Cui P., Shiota M., Nakamura Y., Adachi M., Kitamura K., Tomita K., Kido H.
      Biol. Chem. 384:1483-1495(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.
      Strain: Sprague-DawleyImported.
      Tissue: KidneyImported.

    Entry informationi

    Entry nameiPRS30_RAT
    AccessioniPrimary (citable) accession number: P83748
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: February 16, 2004
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3