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Protein

Serine protease 30

Gene

Prss30

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Selectively cleaves synthetic peptide substrates of trypsin. Activates the epithelial sodium channel ENaC.1 Publication

Enzyme regulationi

Inhibited by aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor. Partially inhibited by PMSF and DFP.1 Publication

pH dependencei

Optimum pH is 9.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei72 – 721Charge relay systemBy similarity
Active sitei122 – 1221Charge relay systemBy similarity
Active sitei223 – 2231Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: UniProtKB
  2. sodium channel regulator activity Source: UniProtKB

GO - Biological processi

  1. proteolysis Source: UniProtKB
  2. sodium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Protein family/group databases

MEROPSiS01.042.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease 30 (EC:3.4.21.-)
Alternative name(s):
Distal intestinal serine protease
Transmembrane serine protease 1
Short name:
TMSP-1
Transmembrane serine protease 8
Gene namesi
Name:Prss30
Synonyms:DispBy similarity, Tmprss8, Tmsp-1, Tmsp11 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi735142. Prss30.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Propeptidei22 – 309Activation peptide1 PublicationSequence AnalysisPRO_0000027864
Chaini31 – 284254Serine protease 30PRO_0000027865Add
BLAST
Propeptidei285 – 30420Removed in mature formCuratedPRO_0000027866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 73By similarityPROSITE-ProRule annotation
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi155 ↔ 229By similarityPROSITE-ProRule annotation
Disulfide bondi185 ↔ 208By similarityPROSITE-ProRule annotation
Disulfide bondi219 ↔ 247By similarityPROSITE-ProRule annotation
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence Analysis
Lipidationi284 – 2841GPI-anchor amidated alanineCurated

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

PRIDEiP83748.

Expressioni

Tissue specificityi

Expressed predominantly in kidney, small intestine and stomach and moderately in thymus, lung, spleen, testis and skin. In the kidney, expressed mainly in collecting duct of renal medulla and cortex.1 Publication

Inductioni

By aldosterone.1 Publication

Gene expression databases

GenevestigatoriP83748.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000037368.

Structurei

3D structure databases

ProteinModelPortaliP83748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 271241Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOVERGENiHBG013304.
InParanoidiP83748.
KOiK09639.
PhylomeDBiP83748.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83748-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESWARCIFL LLLQILTGGR GDILHSGAGK IVGGQDAPEG RWPWQVSLRT
60 70 80 90 100
EKEGHICGGS LIHEVWVLTA AHCFRRPLNS SFYHVKVGGL TLSLTEPHST
110 120 130 140 150
LVAVRNIFVY PTYLWEDASS GDIALLRLDT PLQPSQFSPV CLPQAQAPLT
160 170 180 190 200
PGTVCWVTGW GATHERELAS VLQELAVPLL DSEDCERMYH IGETSLSGKR
210 220 230 240 250
VIQSDMLCAG FVEGQKDSCQ GDSGGPLVCA INSSWIQVGI TSWGIGCARP
260 270 280 290 300
NKPGVYTRVP DYVDWIQRTL AENHSDAYGC RSRASGAYPA LLLVLLAFAL

PESL
Length:304
Mass (Da):33,086
Last modified:February 16, 2004 - v1
Checksum:i825187B7DD70D789
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073023 mRNA. Translation: BAD01655.1.
RefSeqiNP_955403.1. NM_199371.1.
UniGeneiRn.102783.

Genome annotation databases

GeneIDi287106.
KEGGirno:287106.
UCSCiRGD:735142. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073023 mRNA. Translation: BAD01655.1.
RefSeqiNP_955403.1. NM_199371.1.
UniGeneiRn.102783.

3D structure databases

ProteinModelPortaliP83748.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000037368.

Protein family/group databases

MEROPSiS01.042.

Proteomic databases

PRIDEiP83748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi287106.
KEGGirno:287106.
UCSCiRGD:735142. rat.

Organism-specific databases

CTDi30943.
RGDi735142. Prss30.

Phylogenomic databases

eggNOGiCOG5640.
HOVERGENiHBG013304.
InParanoidiP83748.
KOiK09639.
PhylomeDBiP83748.

Miscellaneous databases

NextBioi625497.

Gene expression databases

GenevestigatoriP83748.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of a transmembrane-type serine protease from rat kidney, a new sodium channel activator."
    Okumura Y., Nishikawa M., Cui P., Shiota M., Nakamura Y., Adachi M., Kitamura K., Tomita K., Kido H.
    Biol. Chem. 384:1483-1495(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.
    Strain: Sprague-DawleyImported.
    Tissue: KidneyImported.

Entry informationi

Entry nameiPRS30_RAT
AccessioniPrimary (citable) accession number: P83748
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: February 16, 2004
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.