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P83748

- PRS30_RAT

UniProt

P83748 - PRS30_RAT

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Protein
Serine protease 30
Gene
Prss30, Disp, Tmprss8, Tmsp-1, Tmsp1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Selectively cleaves synthetic peptide substrates of trypsin. Activates the epithelial sodium channel ENaC.1 Publication

Enzyme regulationi

Inhibited by aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor. Partially inhibited by PMSF and DFP.1 Publication

pH dependencei

Optimum pH is 9.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei72 – 721Charge relay system By similarityBy similarity
Active sitei122 – 1221Charge relay system By similarityBy similarity
Active sitei223 – 2231Charge relay system By similarityBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: UniProtKB
  2. sodium channel regulator activity Source: UniProtKB

GO - Biological processi

  1. proteolysis Source: UniProtKB
  2. sodium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Protein family/group databases

MEROPSiS01.042.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease 30 (EC:3.4.21.-)
Alternative name(s):
Distal intestinal serine protease
Transmembrane serine protease 1
Short name:
TMSP-1
Transmembrane serine protease 8
Gene namesi
Name:Prss30
Synonyms:Disp, Tmprss8, Tmsp-1, Tmsp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi735142. Prss30.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Propeptidei22 – 309Activation peptide
PRO_0000027864
Chaini31 – 284254Serine protease 301 Publication
PRO_0000027865Add
BLAST
Propeptidei285 – 30420Removed in mature form Reviewed prediction
PRO_0000027866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 73 By similarityBy similarity
Glycosylationi79 – 791N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi155 ↔ 229 By similarityBy similarity
Disulfide bondi185 ↔ 208 By similarityBy similarity
Disulfide bondi219 ↔ 247 By similarityBy similarity
Glycosylationi232 – 2321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi273 – 2731N-linked (GlcNAc...) Reviewed prediction
Lipidationi284 – 2841GPI-anchor amidated alanine Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

PRIDEiP83748.

Expressioni

Tissue specificityi

Expressed predominantly in kidney, small intestine and stomach and moderately in thymus, lung, spleen, testis and skin. In the kidney, expressed mainly in collecting duct of renal medulla and cortex.1 Publication

Inductioni

By aldosterone.1 Publication

Gene expression databases

GenevestigatoriP83748.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000037368.

Structurei

3D structure databases

ProteinModelPortaliP83748.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 271241Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOVERGENiHBG013304.
InParanoidiP83748.
KOiK09639.
PhylomeDBiP83748.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83748-1 [UniParc]FASTAAdd to Basket

« Hide

MESWARCIFL LLLQILTGGR GDILHSGAGK IVGGQDAPEG RWPWQVSLRT    50
EKEGHICGGS LIHEVWVLTA AHCFRRPLNS SFYHVKVGGL TLSLTEPHST 100
LVAVRNIFVY PTYLWEDASS GDIALLRLDT PLQPSQFSPV CLPQAQAPLT 150
PGTVCWVTGW GATHERELAS VLQELAVPLL DSEDCERMYH IGETSLSGKR 200
VIQSDMLCAG FVEGQKDSCQ GDSGGPLVCA INSSWIQVGI TSWGIGCARP 250
NKPGVYTRVP DYVDWIQRTL AENHSDAYGC RSRASGAYPA LLLVLLAFAL 300
PESL 304
Length:304
Mass (Da):33,086
Last modified:February 16, 2004 - v1
Checksum:i825187B7DD70D789
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB073023 mRNA. Translation: BAD01655.1.
RefSeqiNP_955403.1. NM_199371.1.
UniGeneiRn.102783.

Genome annotation databases

GeneIDi287106.
KEGGirno:287106.
UCSCiRGD:735142. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB073023 mRNA. Translation: BAD01655.1 .
RefSeqi NP_955403.1. NM_199371.1.
UniGenei Rn.102783.

3D structure databases

ProteinModelPortali P83748.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000037368.

Protein family/group databases

MEROPSi S01.042.

Proteomic databases

PRIDEi P83748.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 287106.
KEGGi rno:287106.
UCSCi RGD:735142. rat.

Organism-specific databases

CTDi 30943.
RGDi 735142. Prss30.

Phylogenomic databases

eggNOGi COG5640.
HOVERGENi HBG013304.
InParanoidi P83748.
KOi K09639.
PhylomeDBi P83748.

Miscellaneous databases

NextBioi 625497.

Gene expression databases

Genevestigatori P83748.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a transmembrane-type serine protease from rat kidney, a new sodium channel activator."
    Okumura Y., Nishikawa M., Cui P., Shiota M., Nakamura Y., Adachi M., Kitamura K., Tomita K., Kido H.
    Biol. Chem. 384:1483-1495(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.
    Strain: Sprague-Dawley.
    Tissue: Kidney.

Entry informationi

Entry nameiPRS30_RAT
AccessioniPrimary (citable) accession number: P83748
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: February 16, 2004
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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