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P83748 (PRS30_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine protease 30

EC=3.4.21.-
Alternative name(s):
Distal intestinal serine protease
Transmembrane serine protease 1
Short name=TMSP-1
Transmembrane serine protease 8
Gene names
Name:Prss30
Synonyms:Disp, Tmprss8, Tmsp-1, Tmsp1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Selectively cleaves synthetic peptide substrates of trypsin. Activates the epithelial sodium channel ENaC. Ref.1

Enzyme regulation

Inhibited by aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor. Partially inhibited by PMSF and DFP. Ref.1

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Probable.

Tissue specificity

Expressed predominantly in kidney, small intestine and stomach and moderately in thymus, lung, spleen, testis and skin. In the kidney, expressed mainly in collecting duct of renal medulla and cortex. Ref.1

Induction

By aldosterone. Ref.1

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 9.0.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Propeptide22 – 309Activation peptide
PRO_0000027864
Chain31 – 284254Serine protease 30 Ref.1
PRO_0000027865
Propeptide285 – 30420Removed in mature form Potential
PRO_0000027866

Regions

Domain31 – 271241Peptidase S1

Sites

Active site721Charge relay system By similarity UniProtKB P49863
Active site1221Charge relay system By similarity UniProtKB P49863
Active site2231Charge relay system By similarity UniProtKB P49863

Amino acid modifications

Lipidation2841GPI-anchor amidated alanine Potential
Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2731N-linked (GlcNAc...) Potential
Disulfide bond57 ↔ 73 By similarity UniProtKB P49863
Disulfide bond155 ↔ 229 By similarity UniProtKB P49863
Disulfide bond185 ↔ 208 By similarity UniProtKB P49863
Disulfide bond219 ↔ 247 By similarity UniProtKB P49863

Sequences

Sequence LengthMass (Da)Tools
P83748 [UniParc].

Last modified February 16, 2004. Version 1.
Checksum: 825187B7DD70D789

FASTA30433,086
        10         20         30         40         50         60 
MESWARCIFL LLLQILTGGR GDILHSGAGK IVGGQDAPEG RWPWQVSLRT EKEGHICGGS 

        70         80         90        100        110        120 
LIHEVWVLTA AHCFRRPLNS SFYHVKVGGL TLSLTEPHST LVAVRNIFVY PTYLWEDASS 

       130        140        150        160        170        180 
GDIALLRLDT PLQPSQFSPV CLPQAQAPLT PGTVCWVTGW GATHERELAS VLQELAVPLL 

       190        200        210        220        230        240 
DSEDCERMYH IGETSLSGKR VIQSDMLCAG FVEGQKDSCQ GDSGGPLVCA INSSWIQVGI 

       250        260        270        280        290        300 
TSWGIGCARP NKPGVYTRVP DYVDWIQRTL AENHSDAYGC RSRASGAYPA LLLVLLAFAL 


PESL 

« Hide

References

[1]"Cloning and characterization of a transmembrane-type serine protease from rat kidney, a new sodium channel activator."
Okumura Y., Nishikawa M., Cui P., Shiota M., Nakamura Y., Adachi M., Kitamura K., Tomita K., Kido H.
Biol. Chem. 384:1483-1495(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB073023 mRNA. Translation: BAD01655.1.
RefSeqNP_955403.1. NM_199371.1.
UniGeneRn.102783.

3D structure databases

ProteinModelPortalP83748.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000037368.

Protein family/group databases

MEROPSS01.042.

Proteomic databases

PRIDEP83748.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID287106.
KEGGrno:287106.
UCSCRGD:735142. rat.

Organism-specific databases

CTD30943.
RGD735142. Prss30.

Phylogenomic databases

eggNOGCOG5640.
HOVERGENHBG013304.
InParanoidP83748.
KOK09639.
PhylomeDBP83748.

Gene expression databases

GenevestigatorP83748.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio625497.

Entry information

Entry namePRS30_RAT
AccessionPrimary (citable) accession number: P83748
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: February 16, 2004
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries