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Protein

Serine/threonine-protein kinase WNK1

Gene

Wnk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537, PubMed:23542070).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

By hypertonicity. Activation requires autophosphorylation of Ser-382. Phosphorylation of Ser-378 also promotes increased activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei233 – 2331ATPPROSITE-ProRule annotationBy similarity
Active sitei349 – 3491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi227 – 2359ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: MGI
  • ion transport Source: UniProtKB
  • negative regulation of pancreatic juice secretion Source: MGI
  • negative regulation of phosphatase activity Source: UniProtKB
  • positive regulation of ion transmembrane transporter activity Source: GO_Central
  • positive regulation of systemic arterial blood pressure Source: MGI
  • protein autophosphorylation Source: Ensembl
  • protein phosphorylation Source: MGI
  • regulation of cellular process Source: UniProtKB
  • regulation of ion homeostasis Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Protein kinase inhibitor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase WNK1 (EC:2.7.11.1)
Alternative name(s):
Protein kinase lysine-deficient 1
Protein kinase with no lysine 1
Gene namesi
Name:Wnk1
Synonyms:Hsn2, Prkwnk1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2442092. Wnk1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi368 – 3681D → A: No effect on inhibition of SLC4A4. 1 Publication

Chemistry

ChEMBLiCHEMBL2176799.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23772377Serine/threonine-protein kinase WNK1PRO_0000086820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171PhosphothreonineCombined sources
Modified residuei165 – 1651PhosphoserineBy similarity
Modified residuei172 – 1721PhosphoserineBy similarity
Modified residuei378 – 3781Phosphoserine; by autocatalysisBy similarity
Modified residuei382 – 3821Phosphoserine; by autocatalysisBy similarity
Modified residuei1256 – 12561PhosphoserineBy similarity
Modified residuei1973 – 19731PhosphoserineBy similarity
Modified residuei2006 – 20061PhosphoserineBy similarity
Modified residuei2007 – 20071PhosphoserineBy similarity
Modified residuei2022 – 20221PhosphoserineBy similarity
Modified residuei2024 – 20241PhosphoserineBy similarity
Modified residuei2027 – 20271PhosphoserineCombined sources
Modified residuei2116 – 21161PhosphoserineBy similarity
Modified residuei2265 – 22651PhosphoserineCombined sources
Modified residuei2281 – 22811PhosphoserineCombined sources
Modified residuei2365 – 23651PhosphoserineBy similarity
Modified residuei2367 – 23671PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP83741.
PaxDbiP83741.
PeptideAtlasiP83741.
PRIDEiP83741.

PTM databases

iPTMnetiP83741.
PhosphoSiteiP83741.

Expressioni

Tissue specificityi

Widely expressed in both adult and embryonic tissue, with highest levels observed in the testis and lower levels in heart, lung, kidney, placenta, brain and skeletal muscle. Expressed in pancreatic duct (PubMed:21317537). Two isoforms are expressed in heart, a single shorter isoform in the kidney. Locates to the distal convoluted tubule, the medullary collecting duct and the cortical collecting duct of the kidney. HSN2-containing isoform 2 and isoform 3 are restricted to the nervous system, expressed preferentially in sensory neurons than in motor neurons and in general more abundant in axons than in cell bodies (at protein level). In the DRG, predominantly expressed in the satellite cells that envelop sensory neurons, but low expression also observed in the cell bodies of neurons (at protein level). In the sciatic nerve, expressed in the Schwann cells that surround axons and in a mosaic distribution of axons (at protein level). In the spinal cord, expressed in superficial layers (LI and LII), as well as in the fibers of the Lissauer tract (at protein level). Also detected in the axon fibers of dorsolateral funiculus and lateral funiculus (at protein level).4 Publications

Gene expression databases

BgeeiENSMUSG00000045962.
CleanExiMM_WNK1.
ExpressionAtlasiP83741. baseline and differential.
GenevisibleiP83741. MM.

Interactioni

Subunit structurei

Interacts with SYT2. Interacts with KLHL3, WNK3 and WNK4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231244. 1 interaction.
STRINGi10090.ENSMUSP00000086017.

Structurei

3D structure databases

ProteinModelPortaliP83741.
SMRiP83741. Positions 210-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini221 – 479259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0584. Eukaryota.
ENOG410XQWZ. LUCA.
GeneTreeiENSGT00800000124049.
HOVERGENiHBG048577.
InParanoidiP83741.
KOiK08867.
OMAiHERPVSF.
OrthoDBiEOG091G06LL.
PhylomeDBiP83741.
TreeFamiTF315363.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P83741-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDGAAEKQS GTPGFLTPPA PVPKNGSSSD SSVGEKLGAT VADSGVGRTE
60 70 80 90 100
EYRRRRHTMD KDSRGAAATT TPTEHRFFRR SVICDSNATA LELPGLPLSI
110 120 130 140 150
PQPSVPAVVP QSAPPEPHRE ETLTATVASQ VSQQPSAAAS PGEQAVVGSA
160 170 180 190 200
TTTVPSSTSK DRPVSQPSLV GSKEEPPPSR SGSGSGGASA KEAQEDRSQQ
210 220 230 240 250
QDDIEELETK AVGMSNDGRF LKFDIEIGRG SFKTVYKGLD TETTVEVAWC
260 270 280 290 300
ELQDRKLTKS ERQRFKEEAE MLKGLQHPNI VRFYDSWEST VKGKKCIVLV
310 320 330 340 350
TELMTSGTLK TYLKRFKVMK IKVLRSWCRQ ILKGLQFLHT RTPPIIHRDL
360 370 380 390 400
KCDNIFITGP TGSVKIGDLG LATLKRASFA KSVIGTPEFM APEMYEEKYD
410 420 430 440 450
ESVDVYAFGM CMLEMATSEY PYSECQNAAQ IYRRVTSGVK PASFDKVAIP
460 470 480 490 500
EVKEIIEGCI RQNKDERYSI KDLLNHAFFQ EETGVRVELA EEDDGEKIAI
510 520 530 540 550
KLWLRIEDIK KLKGKYKDNE AIEFSFDLER DVPEDVAQEM VESGYVCEGD
560 570 580 590 600
HKTMAKAIKD RVSLIKRKRE QRQLVREEQE KRKQEESSFK QQNEQQASVS
610 620 630 640 650
QAGIQQLSAA STGIPTAPAT SASVSTQVEP EEPEADQHQQ LQYQQPSISV
660 670 680 690 700
LSDGTIDSGQ GSSVFTESRV SSQQTVSYGS QHEQAHSTGT APGHTVSSIQ
710 720 730 740 750
AQSQPHGVYP PSSMAQGQNQ GQPSSSLAGV LSSQPIQHPQ QQGIQPTVPS
760 770 780 790 800
QQAVQYSLPQ AASSSEGTTA QPVSQPQVSA GTQLPVSQTV ATVQGEPHIP
810 820 830 840 850
VSTQPSVVPV HSGAHFLPMG QPIPTSLLPQ YPVSQIPIST PHVSTAQTGF
860 870 880 890 900
SSVPITMAAG INQPLLTLAS SATASSIPGG SPVVPNQLPT LLQPVNQLQS
910 920 930 940 950
QVHPQLLQPT TVQSIGIPAN LGQAAEGPLP SGDVLYQGFP SRLPPQYPGD
960 970 980 990 1000
SNIAPSSNVA SVCIHSTVLA PPSMPTEALA TQGYFPTVVQ PYVESTPLVP
1010 1020 1030 1040 1050
MGSVGGQVQV SQPAVSLTQQ PPTTSSQQAV LESTQGVSQA APPEQTPITQ
1060 1070 1080 1090 1100
SQPTQPVPLV TSADSAHSDV ASGMSDGNEN APSSSGRHEG RTTKRHYRKS
1110 1120 1130 1140 1150
VRSRSRHEKT SRPKLRILNV SNKGDRVVEC QLETHNRKMV TFKFDLDGDN
1160 1170 1180 1190 1200
PEEIATIMVN NDFILAIERE SFVAQVREII EKADEMLSED VSVEPEGDQG
1210 1220 1230 1240 1250
LESLQGKDDY GFPGSQKLEG EFKQPIAVSS MPQQIGVPTS SLTQVVHSAG
1260 1270 1280 1290 1300
RRFIVSPVPE SRLRESKVFT SDISDPVVAS TSQAPGMNLS HSASSLSLQQ
1310 1320 1330 1340 1350
AFSELKHGQM TEGPNTAPPN FNHMAGPTFS PFLASIAGVQ TVAASTPSVS
1360 1370 1380 1390 1400
VPITSSPLND ISTSVMQSET ALPTEKGIVG VTTTSTGVVA SGGLTTMSVS
1410 1420 1430 1440 1450
ESPTSSSAVS SSTVPAVVTV STPSQPVQAS TSGSIASSTG SFPPGTFSTT
1460 1470 1480 1490 1500
TATTMGSVVA PDAKPPTVLL QQVASNTAGV AIVTSVSTTT PFPGMASQPS
1510 1520 1530 1540 1550
LPLSSSTSAP TLAETMVVSA HSLDKASHSS TAGLGLSFCA PSSSSSSGTA
1560 1570 1580 1590 1600
VSTSVSQPGM VHPLVISSAV VSTPGLPQPV VPTSTPLLPQ VPNIPPLVQP
1610 1620 1630 1640 1650
VVNVPAVQQT LIHSQPQPAL LPNQPHTHCP EMDADTQSKA PGIDDIKTLE
1660 1670 1680 1690 1700
EKLRSLFSEH SSSGTQHASV SLETPLVVET TVTPGITTTA VAPSKLMTST
1710 1720 1730 1740 1750
TSTCLPPTSL PLGAAGMPVM PVGTPGQVST PGTHASAPVG TATGVKPGTT
1760 1770 1780 1790 1800
PPKPTKTVVP PVGTELSAGT VPCEQLPPFP GPSLIQSQQP LEDLDAQLRR
1810 1820 1830 1840 1850
TLSPETITVA PAVGPLSTMS STTVTEAGTR LQKDGTEGHV TATSSGAGVV
1860 1870 1880 1890 1900
KMGRFQVSVT MDDAQKERKN RSEDTKSVHF ESSTSESSVL SSSSPESTLV
1910 1920 1930 1940 1950
KPEPNGISIS GISLDVPDST HKAPTPEAKS DAGQPTKVGR FQVTTTANKV
1960 1970 1980 1990 2000
GRFSVSRTED KVTELKKEGP VTSPPFRDSE QTVIPAVIPK KEKPELAEPS
2010 2020 2030 2040 2050
HLNGPSSDLE AAFLSRGTED GSGSPHSPPH LCSKSLPVQN LSQSLSNSFN
2060 2070 2080 2090 2100
SSYMSSDNES DIEDEDLRLE LRRLREKHLK EIQDLQSRQK HEIESLYTKL
2110 2120 2130 2140 2150
GKVPPAVIIP PAAPLSGRRR RPTKSKGSKS SRSSSLGNKS PQLSGNLSGQ
2160 2170 2180 2190 2200
SGTSVLHPQQ TLHPAGNTPE TGHNQLLQPL KPSPSSDNLY SAFTSDGAIS
2210 2220 2230 2240 2250
VPSLSAPGQG TSSTNTVGGT VSSQAAQAQP PAMTSSRKGT FTDDLHKLVD
2260 2270 2280 2290 2300
NWARDAMNLS GRRGSKGHMN YEGPGMARKF SAPGQLCVPM TSNLGGSTPI
2310 2320 2330 2340 2350
SAASATSLGH FTKSMCPPQQ YGFPPAPFGT QWSGTGGPAP QPLGQFQPVG
2360 2370
TASLQNFNIS NLQKSISNPP GSNLRTT
Length:2,377
Mass (Da):250,934
Last modified:November 30, 2010 - v2
Checksum:i0C4D288CCE50B8C6
GO
Isoform 2 (identifier: P83741-2) [UniParc]FASTAAdd to basket
Also known as: Brain and spinal cord variant

The sequence of this isoform differs from the canonical sequence as follows:
     715-1032: AQGQNQGQPS...TTSSQQAVLE → PRRGRSMSVC...SQPQVSAGTQ

Note: This isoform which includes the HSN2 exon has been identified in human and mouse. The sequence shown here is the result of gene prediction.
Show »
Length:2,626
Mass (Da):279,185
Checksum:i1933C3D136EE2103
GO
Isoform 3 (identifier: P83741-3) [UniParc]FASTAAdd to basket
Also known as: Dorsal root ganglia and sciatic nerve variant, DRG and sciatic nerve variant

The sequence of this isoform differs from the canonical sequence as follows:
     715-936: AQGQNQGQPS...GPLPSGDVLY → PQSMAHPCGG...VSQPQVSAGT

Note: This isoform which includes the HSN2 exon has been identified in human and mouse. The sequence shown here is the result of gene prediction.
Show »
Length:2,635
Mass (Da):279,763
Checksum:i8E2B08C9DCB624BF
GO
Isoform 4 (identifier: P83741-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     784-1032: Missing.

Note: No experimental confirmation available.
Show »
Length:2,128
Mass (Da):225,405
Checksum:iA414E93D815771CE
GO
Isoform 5 (identifier: P83741-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     784-937: Missing.
     1787-1814: Missing.

Note: No experimental confirmation available.
Show »
Length:2,195
Mass (Da):232,203
Checksum:i43A9B0F75D83925D
GO
Isoform 6 (identifier: P83741-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     784-788: LPVSQ → VNSNF
     789-2377: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:788
Mass (Da):86,037
Checksum:i75A4C9BD9B35580E
GO

Sequence cautioni

The sequence AAI46010 differs from that shown.Probable cloning artifact.Curated
The sequence AAI46012 differs from that shown.Probable cloning artifact.Curated
The sequence BAD32213 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence DAA04493 differs from that shown. Reason: Erroneous gene model prediction. Includes 3' and 3' intronic sequences.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1220 – 12201G → R in AAQ77243 (PubMed:14514722).Curated
Sequence conflicti1230 – 12301S → C in AAQ77243 (PubMed:14514722).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei715 – 1032318AQGQN…QAVLE → PRRGRSMSVCVPHLSAVPSL SRISPSAPSTPPPVLSAPLC PSLLRTAPEETFAEKLSKAL ESVLPMHSASQRKHRRSSLP SLFVTTPQSMAHPCGGTPTY PESQIFFPTIHERPVSFSPP PTCPPKVAISQRRKSTSFLE AQTRHFQPLLRTVGQNHLPP GSSPTNWTPEAIVMLGATAN RVNRELCEMQVQPVFEPTQI YSDYRPGLVLAEEAHYFIPQ ETVYLAGVHYQAQVAGQYEG ISYNSPVLSSPMKQISEQKP VPGGPASSSVFEFPSGQAFL VGHLQNLRLDSGPSPASPLS SISAPNSTDATHLKFHPVFV PHSAPAVLTNSNENRSNCVF EFHAQTPSSSGEGGGILPQR VYRNRQVAVDSNQEELSPQS VGLHCHLQPVTEEQRNNHAP ELTISVVEPMGQIWPIGSPE YSSDSSQITSSDLSDFQSPP PTGGTAAPFGSDVSLPFIRL PQTVLQESPLFFCFPQGTTS QQVLSASYSSGGSTLHPQAQ GQNQGQPSSSLAGVLSSQPI QHPQQQGIQPTVPSQQAVQY SLPQAASSSEGTTAQPVSQP QVSAGTQ in isoform 2. CuratedVSP_040271Add
BLAST
Alternative sequencei715 – 936222AQGQN…GDVLY → PQSMAHPCGGTPTYPESQIF FPTIHERPVSFSPPPTCPPK VAISQRRKSTSFLEAQTRHF QPLLRTVGQNHLPPGSSPTN WTPEAIVMLGATANRVNREL CEMQVQPVFEPTQIYSDYRP GLVLAEEAHYFIPQETVYLA GVHYQAQVAGQYEGISYNSP VLSSPMKQISEQKPVPGGPA SSSVFEFPSGQAFLVGHLQN LRLDSGPSPASPLSSISAPN STDATHLKFHPVFVPHSAPA VLTNSNENRSNCVFEFHAQT PSSSGEGGGILPQRVYRNRQ VAVDSNQEELSPQSVGLHCH LQPVTEEQRNNHAPELTISV VEPMGQIWPIGSPEYSSDSS QITSSDLSDFQSPPPTGGTA APFGSDVSLPFIRLPQTVLQ ESPLFFCFPQGTTSQQVLSA SYSSGGSTLHPQAQGQNQGQ PSSSLAGVLSSQPIQHPQQQ GIQPTVPSQQAVQYSLPQAA SSSEGTTAQPVSQPQVSAGT in isoform 3. CuratedVSP_040272Add
BLAST
Alternative sequencei784 – 1032249Missing in isoform 4. 1 PublicationVSP_040273Add
BLAST
Alternative sequencei784 – 937154Missing in isoform 5. 1 PublicationVSP_040274Add
BLAST
Alternative sequencei784 – 7885LPVSQ → VNSNF in isoform 6. 1 PublicationVSP_040275
Alternative sequencei789 – 23771589Missing in isoform 6. 1 PublicationVSP_040276Add
BLAST
Alternative sequencei1787 – 181428Missing in isoform 5. 1 PublicationVSP_040277Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY309076 mRNA. Translation: AAQ77243.1.
AK172935 mRNA. Translation: BAD32213.1. Different initiation.
AC113092 Genomic DNA. No translation available.
AC117667 Genomic DNA. No translation available.
BC056761 mRNA. Translation: AAH56761.1.
BC138445 mRNA. Translation: AAI38446.1.
BC145282 mRNA. Translation: AAI45283.1.
BC146009 mRNA. Translation: AAI46010.1. Sequence problems.
BC146011 mRNA. Translation: AAI46012.1. Sequence problems.
BC171955 mRNA. Translation: AAI71955.1.
AK133738 mRNA. Translation: BAE21813.1.
BK004107 Genomic DNA. Translation: DAA04493.1. Sequence problems.
CCDSiCCDS20478.1. [P83741-1]
CCDS51888.1. [P83741-5]
CCDS57444.1. [P83741-3]
CCDS57445.1. [P83741-2]
RefSeqiNP_001171949.1. NM_001185020.1. [P83741-4]
NP_001171950.1. NM_001185021.1. [P83741-5]
NP_001186012.1. NM_001199083.1. [P83741-2]
NP_001186013.1. NM_001199084.1. [P83741-3]
NP_941992.2. NM_198703.3. [P83741-1]
UniGeneiMm.333349.

Genome annotation databases

EnsembliENSMUST00000060043; ENSMUSP00000063001; ENSMUSG00000045962. [P83741-1]
ENSMUST00000088644; ENSMUSP00000086017; ENSMUSG00000045962. [P83741-3]
ENSMUST00000088646; ENSMUSP00000086019; ENSMUSG00000045962. [P83741-5]
ENSMUST00000177761; ENSMUSP00000136777; ENSMUSG00000045962. [P83741-2]
ENSMUST00000203030; ENSMUSP00000145304; ENSMUSG00000045962. [P83741-4]
GeneIDi232341.
KEGGimmu:232341.
UCSCiuc009dmt.2. mouse. [P83741-1]
uc009dmu.2. mouse. [P83741-5]
uc009dmv.2. mouse. [P83741-6]
uc012ers.1. mouse. [P83741-3]
uc012ert.1. mouse. [P83741-2]
uc012eru.1. mouse. [P83741-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY309076 mRNA. Translation: AAQ77243.1.
AK172935 mRNA. Translation: BAD32213.1. Different initiation.
AC113092 Genomic DNA. No translation available.
AC117667 Genomic DNA. No translation available.
BC056761 mRNA. Translation: AAH56761.1.
BC138445 mRNA. Translation: AAI38446.1.
BC145282 mRNA. Translation: AAI45283.1.
BC146009 mRNA. Translation: AAI46010.1. Sequence problems.
BC146011 mRNA. Translation: AAI46012.1. Sequence problems.
BC171955 mRNA. Translation: AAI71955.1.
AK133738 mRNA. Translation: BAE21813.1.
BK004107 Genomic DNA. Translation: DAA04493.1. Sequence problems.
CCDSiCCDS20478.1. [P83741-1]
CCDS51888.1. [P83741-5]
CCDS57444.1. [P83741-3]
CCDS57445.1. [P83741-2]
RefSeqiNP_001171949.1. NM_001185020.1. [P83741-4]
NP_001171950.1. NM_001185021.1. [P83741-5]
NP_001186012.1. NM_001199083.1. [P83741-2]
NP_001186013.1. NM_001199084.1. [P83741-3]
NP_941992.2. NM_198703.3. [P83741-1]
UniGeneiMm.333349.

3D structure databases

ProteinModelPortaliP83741.
SMRiP83741. Positions 210-572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231244. 1 interaction.
STRINGi10090.ENSMUSP00000086017.

Chemistry

ChEMBLiCHEMBL2176799.

PTM databases

iPTMnetiP83741.
PhosphoSiteiP83741.

Proteomic databases

MaxQBiP83741.
PaxDbiP83741.
PeptideAtlasiP83741.
PRIDEiP83741.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060043; ENSMUSP00000063001; ENSMUSG00000045962. [P83741-1]
ENSMUST00000088644; ENSMUSP00000086017; ENSMUSG00000045962. [P83741-3]
ENSMUST00000088646; ENSMUSP00000086019; ENSMUSG00000045962. [P83741-5]
ENSMUST00000177761; ENSMUSP00000136777; ENSMUSG00000045962. [P83741-2]
ENSMUST00000203030; ENSMUSP00000145304; ENSMUSG00000045962. [P83741-4]
GeneIDi232341.
KEGGimmu:232341.
UCSCiuc009dmt.2. mouse. [P83741-1]
uc009dmu.2. mouse. [P83741-5]
uc009dmv.2. mouse. [P83741-6]
uc012ers.1. mouse. [P83741-3]
uc012ert.1. mouse. [P83741-2]
uc012eru.1. mouse. [P83741-4]

Organism-specific databases

CTDi65125.
MGIiMGI:2442092. Wnk1.

Phylogenomic databases

eggNOGiKOG0584. Eukaryota.
ENOG410XQWZ. LUCA.
GeneTreeiENSGT00800000124049.
HOVERGENiHBG048577.
InParanoidiP83741.
KOiK08867.
OMAiHERPVSF.
OrthoDBiEOG091G06LL.
PhylomeDBiP83741.
TreeFamiTF315363.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

ChiTaRSiWnk1. mouse.
PROiP83741.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045962.
CleanExiMM_WNK1.
ExpressionAtlasiP83741. baseline and differential.
GenevisibleiP83741. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWNK1_MOUSE
AccessioniPrimary (citable) accession number: P83741
Secondary accession number(s): A6H6V1
, B2RRJ7, B7ZNJ4, Q3UZP3, Q6A083, Q6IFS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: November 30, 2010
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Cys-250 is present instead of the conserved Lys which is expected to be an active site residue. Lys-233 appears to fulfill the required catalytic function.By similarity
It is uncertain whether Met-1 or Met-214 is the initiator.Curated
HSN2 was originally thought to be an intronless gene lying within a WNK1 gene intron. It has been shown to be a nervous system-specific exon of WNK1 included in isoform 2 and isoform 3 (PubMed:18521183).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.