P83736 (ADOK_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine kinase
Short name=AK
EC=2.7.1.20

Gene names

Name:	adoK
Synonyms:	cbhK
Ordered Locus Names:	Mb2225c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	ATP dependent phosphorylation of adenosine to monophosphate derivatives By similarity.
Catalytic activity	ATP + adenosine = ADP + AMP.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activity is stimulated in the presence of potassium, resulting in a 13-fold increase in the catalytic efficiency for adenosine By similarity.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.
Subunit structure	Homodimer Probable.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Purine salvage
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosine kinase activity Inferred from electronic annotation. Source: EC phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 324	323	Adenosine kinase		PRO_0000080063
Sites
	Active site	257	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P83736 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0C072206A3210A1D	FASTA	324	34,472
	10 20 30 40 50 60 MTIAVTGSIA TDHLMRFPGR FSEQLLPEHL HKVSLSFLVD DLVMHRGGVA GNMAFAIGVL 70 80 90 100 110 120 GGEVALVGAA GADFADYRDW LKARGVNCDH VLISETAHTA RFTCTTDVDM AQIASFYPGA 130 140 150 160 170 180 MSEARNIKLA DVVSAIGKPE LVIIGANDPE AMFLHTEECR KLGLAFAADP SQQLARLSGE 190 200 210 220 230 240 EIRRLVNGAA YLFTNDYEWD LLLSKTGWSE ADVMAQIDLR VTTLGPKGVD LVEPDGTTIH 250 260 270 280 290 300 VGVVPETSQT DPTGVGDAFR AGFLTGRSAG LGLERSAQLG SLVAVLVLES TGTQEWQWDY 310 320 EAAASRLAGA YGEHAAAEIV AVLA

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248341 Genomic DNA. Translation: CAD97078.1.
RefSeq	NP_855874.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P83736.
SMR	P83736. Positions 1-324.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000016180; EBMYCP00000016015; EBMYCG00000016175.
GeneID	1091347.
GenomeReviews	Gene locus Mb2225c in contig BX248333_GR.
KEGG	mbo:Mb2225c.
PATRIC	18006590. VBIMycBov88188_2441.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016732.
HOGENOM	HBG296008.
OMA	FTAQAFI.
ProtClustDB	CLSK872029.
Enzyme and pathway databases
BioCyc	MBOV233413:MB2225C-MONOMER.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. [Graphical view]
KO	K00856.
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PROSITE	PS00583. PFKB_KINASES_1. 1 hit. PS00584. PFKB_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADOK_MYCBO

Accession

Primary (citable) accession number: P83736
Secondary accession number(s): Q10391

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program