Reviewed, UniProtKB/Swiss-Prot P83734 (ADOK_MYCTU)

Names and origin

Protein names

Recommended name:
    Adenosine kinase
      Short name=AK
    EC=2.7.1.20

Gene names

Name:	adoK
Synonyms:	cbhK
Ordered Locus Names:	Rv2202c, MT2258
ORF Names:	MTCY190.13c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ATP dependent phosphorylation of adenosine to monophosphate derivatives By similarity.
Catalytic activity	ATP + adenosine = ADP + AMP.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activity is stimulated in the presence of potassium, resulting in a 13-fold increase in the catalytic efficiency for adenosine By similarity.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
Subunit structure	Homodimer Probable.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Purine salvage
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosine kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 324

323

Adenosine kinase

PRO_0000080064

Sites

Active site

257

1

By similarity

Secondary structure

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324

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Beta strand

10 – 16

7

Helix

22 – 25

4

Helix

30 – 32

3

Beta strand

34 – 38

5

Beta strand

40 – 47

8

Helix

49 – 59

11

Beta strand

63 – 67

5

Helix

72 – 74

3

Helix

75 – 82

8

Turn

83 – 85

3

Beta strand

100 – 106

7

Beta strand

112 – 117

6

Helix

119 – 126

8

Helix

129 – 136

8

Beta strand

140 – 146

7

Helix

149 – 162

14

Beta strand

166 – 169

4

Helix

171 – 175

5

Helix

179 – 183

5

Turn

184 – 188

5

Beta strand

190 – 195

6

Helix

196 – 206

11

Helix

210 – 214

5

Beta strand

220 – 223

4

Helix

225 – 227

3

Beta strand

229 – 232

4

Beta strand

238 – 241

4

Helix

255 – 268

14

Helix

273 – 288

16

Beta strand

290 – 294

5

Helix

300 – 311

12

Helix

313 – 320

8

Sequences

	Sequence		Length	Mass (Da)
	P83734-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0C072206A3210A1D	FASTA	324	34,472
	10 20 30 40 50 60 MTIAVTGSIA TDHLMRFPGR FSEQLLPEHL HKVSLSFLVD DLVMHRGGVA GNMAFAIGVL 70 80 90 100 110 120 GGEVALVGAA GADFADYRDW LKARGVNCDH VLISETAHTA RFTCTTDVDM AQIASFYPGA 130 140 150 160 170 180 MSEARNIKLA DVVSAIGKPE LVIIGANDPE AMFLHTEECR KLGLAFAADP SQQLARLSGE 190 200 210 220 230 240 EIRRLVNGAA YLFTNDYEWD LLLSKTGWSE ADVMAQIDLR VTTLGPKGVD LVEPDGTTIH 250 260 270 280 290 300 VGVVPETSQT DPTGVGDAFR AGFLTGRSAG LGLERSAQLG SLVAVLVLES TGTQEWQWDY 310 320 EAAASRLAGA YGEHAAAEIV AVLA

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BX842579 Genomic DNA. Translation: CAA94245.1.
AE000516 Genomic DNA. Translation: AAK46544.1.

PIR

C70785.

RefSeq

NP_216718.1.
NP_336730.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PKF	X-ray	1.50	A/B	1-324	[»]
2PKK	X-ray	1.93	A	1-324	[»]
2PKM	X-ray	1.90	A	1-324	[»]
2PKN	X-ray	1.90	A	1-324	[»]

ModBase

Search...

Genome annotation databases

GeneID

888551.
924168.

GenomeReviews

Gene locus MT2258 in contig AE000516_GR.
Gene locus Rv2202c in contig AL123456_GR.

KEGG

mtc:MT2258.
mtu:Rv2202c.

TIGR

MT2258.

Organism-specific databases

TubercuList

Rv2202c.

Phylogenomic databases

HOGENOM

P83734.

OMA

CGSLAFD.

Enzyme and pathway databases

BRENDA

2.7.1.20. 809.

Family and domain databases

InterPro

IPR011611. Carb/pur_kinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
[Graphical view]

Pfam

PF00294. PfkB. 1 hit.
[Graphical view]

PROSITE

PS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ADOK_MYCTU

Accession

Primary (citable) accession number: P83734
Secondary accession number(s): Q10391

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)