Reviewed, UniProtKB/Swiss-Prot P83726 (IDHC_NAEFO)

Names and origin

Protein names	Recommended name:     Isocitrate dehydrogenase [NADP]       Short name=IDH     EC=1.1.1.42 Alternative name(s):     Oxalosuccinate decarboxylase     NADP(+)-specific ICDH     IDP     NF033
Organism	Naegleria fowleri (Brain eating amoeba)
Taxonomic identifier	5763 [NCBI]
Taxonomic lineage	Eukaryota › Heterolobosea › Schizopyrenida › Vahlkampfiidae › Naegleria

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Manganese or magnesium By similarity.
Subcellular location	CytoplasmBy similarity.
Miscellaneous	On the 2D-gel the determined pI of this protein is: 6.8, its MW is: 32.3 kDa.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium Manganese NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	Isocitrate dehydrogenase [NADP]		PRO_0000083582
Experimental info
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	P83726-1 [UniParc]. Last modified January 16, 2004. Version 1. Checksum: 4A8244E9BA01D677	FASTA	20	2,215
	10 20 AXFQKIKVAN PVVELDGDEM

References

[1]	"Comparative study of protein profiles on pathogenic and nonpathogenic Naegleria species by 2D-PAGE." Omura M., Furushima-Shimogawara R., Izumiyama S., Endo T. Submitted (DEC-2003) to UniProtKB Cited for: PROTEIN SEQUENCE. Strain: Nf 66.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR004790. IsoCit_DHase_NADP-dep_euk. IPR001804. IsoCit_IM_DHase. [Graphical view]
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHC_NAEFO

Accession

Primary (citable) accession number: P83726

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)