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Protein

Pre-protein-C8

Gene

proC8

Organism
Halobacterium sp. (strain AS7092)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Halocin-C8: Has antibacterial activity against a wide variety of haloarchaeons. Causes cell lysis and death, possibly by disrupting the cell wall (PubMed:12811620).1 Publication
Immunity protein HalI: Acts as a immunity protein for halocin-C8. Able to block the halocin-C8 activity by sequestering the activity of halocin-C8 through specific and direct binding (PubMed:15978083, PubMed:18658263).2 Publications

GO - Biological processi

  • cytolysis Source: UniProtKB-KW
  • defense response to bacterium Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi9.A.45.1.1. the archaeocin/halocin c8 (halc8) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-protein-C8
Cleaved into the following 2 chains:
Gene namesi
Name:proC8
OrganismiHalobacterium sp. (strain AS7092)
Taxonomic identifieri245739 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium

Subcellular locationi

Halocin-C8 :
Immunity protein HalI :
  • Cell membrane

  • Note: Exclusively localizes to the cell membrane.1 Publication

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi61 – 611A → D: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi62 – 621R → A: Slightly decreased immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi63 – 631E → A: Slightly decreased immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi64 – 641L → A: Impaired immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi65 – 651A → P: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi66 – 661K → P: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi67 – 671T → A: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi68 – 681P → A: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi69 – 691A → V: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi70 – 701F → A: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi71 – 711R → A: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi72 – 721E → A: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi73 – 731L → A: Impaired immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi74 – 741A → V: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication
Mutagenesisi75 – 751Q → A: No effect on immunity function of Immunity protein HalI and interaction with Halocin-C8. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini41 – 207167Immunity protein HalIPRO_0000429048Add
BLAST
Chaini208 – 28376Halocin-C8PRO_0000195165Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.PROSITE-ProRule annotation1 Publication

Expressioni

Inductioni

Increases to maximal levels upon transition from exponential to stationary phase.1 Publication

Interactioni

Subunit structurei

Immunity protein HalI interacts with Halocin-C8; the interaction is direct.1 Publication

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni61 – 7515Helix-loop-helix (HLH) regionAdd
BLAST

Domaini

The helix-loop-helix (HLH) region is essential for immunity function of Immunity protein HalI and interaction with Halocin-C8.1 Publication

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR031033. Halocin_C8_dom.
IPR006311. TAT_signal.
[Graphical view]
TIGRFAMsiTIGR04449. halocin_C8_dom. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83716-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEDNNTSEE SGRINRRNVL KTVGAAGLFA AGSTGMAAAA DSLSQAEEPK
60 70 80 90 100
LLTGTEKRTL ARELAKTPAF RELAQRARAD GAQIRSDADS IVAGYARGED
110 120 130 140 150
FAREVVQYDL ENLTDAAEAS IVIGRNPETG EIEVANLDYY YETDDGVLDE
160 170 180 190 200
VHRFEPTNAS ETDGVQSAAT SDGATVIAVD TDAIREAQNS EIDVDESSPS
210 220 230 240 250
NAAPTPADID ITGCSACKYA AGQVCTIGCS AAGGFICGLL GITIPVAGLS
260 270 280
CLGFVEIVCT VADEYSGCGD AVAKEACNRA GLC
Length:283
Mass (Da):29,379
Last modified:May 14, 2014 - v2
Checksum:iFF5B95B523D29A12
GO

Mass spectrometryi

Molecular mass is 7427.1 Da from positions 208 - 283. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ137141 Genomic DNA. Translation: AAQ82548.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ137141 Genomic DNA. Translation: AAQ82548.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi9.A.45.1.1. the archaeocin/halocin c8 (halc8) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR031033. Halocin_C8_dom.
IPR006311. TAT_signal.
[Graphical view]
TIGRFAMsiTIGR04449. halocin_C8_dom. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHAC8_HALAS
AccessioniPrimary (citable) accession number: P83716
Secondary accession number(s): Q6JSL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 14, 2014
Last modified: January 20, 2016
This is version 27 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Halocin-C8: Is quite robust, as it can be desalted, boiled, subjected to organic solvents, and stored at 4 degrees Celsius for extended periods without losing activity.1 Publication

Keywords - Technical termi

Direct protein sequencing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.