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P83692 (GANA_THIHE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arabinogalactan endo-1,4-beta-galactosidase

EC=3.2.1.89
Alternative name(s):
Endo-1,4-beta-galactanase
Short name=Galactanase
OrganismThielavia heterothallica (Myceliophthora thermophila)
Taxonomic identifier78579 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesChaetomiaceaeMyceliophthora

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans. Ref.1

Miscellaneous

Has a pH range of 5.5-8.5 with optimum of 7.0; and a temperature optimum of 65 degrees Celsius at pH 6.5. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 53 family. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Arabinogalactan endo-1,4-beta-galactosidase
PRO_0000057706

Sites

Active site1351Proton donor By similarity UniProtKB P48841
Active site2451Nucleophile By similarity UniProtKB P48841

Amino acid modifications

Glycosylation1111N-linked (GlcNAc...) Ref.1

Experimental info

Mutagenesis90 – 912AH → SD: Lowers pH profile by 0.5 units. Ref.1

Secondary structure

.................................................... 332
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P83692 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 71CA092FDB1D43DC

FASTA33236,812
        10         20         30         40         50         60 
ALTYRGVDWS SVVVEERAGV SYKNTNGNAQ PLENILAANG VNTVRQRVWV NPADGNYNLD 

        70         80         90        100        110        120 
YNIAIAKRAK AAGLGVYIDF HYSDTWADPA HQTMPAGWPS DIDNLSWKLY NYTLDAANKL 

       130        140        150        160        170        180 
QNAGIQPTIV SIGNEIRAGL LWPTGRTENW ANIARLLHSA AWGIKDSSLS PKPKIMIHLD 

       190        200        210        220        230        240 
NGWDWGTQNW WYTNVLKQGT LELSDFDMMG VSFYPFYSSS ATLSALKSSL DNMAKTWNKE 

       250        260        270        280        290        300 
IAVVETNWPI SCPNPRYSFP SDVKNIPFSP EGQTTFITNV ANIVSSVSRG VGLFYWEPAW 

       310        320        330 
IHNANLGSSC ADNTMFSQSG QALSSLSVFQ RI 

« Hide

References

[1]"Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum."
Le Nours J., Ryttersgaard C., Lo Leggio L., Oestergaard P.R., Borchert T.V., Christensen L.L.H., Larsen S.
Protein Sci. 12:1195-1204(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), CATALYTIC ACTIVITY, GLYCOSYLATION AT ASN-111, MUTAGENESIS OF 90-ASP-HIS-91.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HJSX-ray1.87A/B/C/D1-332[»]
1HJUX-ray2.15A/B/C/D1-332[»]
ProteinModelPortalP83692.
SMRP83692. Positions 1-332.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH53. Glycoside Hydrolase Family 53.
mycoCLAPGAN53A_THIHE.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP83692.

Entry information

Entry nameGANA_THIHE
AccessionPrimary (citable) accession number: P83692
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 15, 2003
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries