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P83689

- OCP_ARTMA

UniProt

P83689 - OCP_ARTMA

Protein

Orange carotenoid-binding protein

Gene
N/A
Organism
Arthrospira maxima (Spirulina maxima)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy.1 Publication

    Absorptioni

    Abs(max)=494 nm2 Publications

    Also shows other maxima at 465 nm and 275 nm. The red form (RCP) shows a broad peak at 500 nm.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531Carotenoid1 Publication
    Binding sitei227 – 2271Carotenoid1 Publication
    Binding sitei277 – 2771Chloride1 Publication
    Binding sitei286 – 2861Carotenoid1 Publication
    Binding sitei290 – 2901Carotenoid1 Publication
    Binding sitei305 – 3051Carotenoid1 Publication

    GO - Molecular functioni

    1. chloride ion binding Source: InterPro

    GO - Biological processi

    1. light absorption Source: InterPro
    2. transport Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Orange carotenoid-binding protein
    Short name:
    OCP
    Cleaved into the following chain:
    OrganismiArthrospira maxima (Spirulina maxima)
    Taxonomic identifieri129910 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesArthrospira

    Subcellular locationi

    Cellular thylakoid membrane By similarity1 Publication
    Note: Associated with the phycobilisome.By similarity

    GO - Cellular componenti

    1. phycobilisome Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Phycobilisome, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 317316Orange carotenoid-binding protein1 PublicationPRO_0000282350Add
    BLAST
    Chaini16 – ?Red carotenoid-binding proteinBy similarityPRO_0000282351

    Post-translational modificationi

    Proteolytically cleaved into a red 16.7 kDa form named red carotenoid-binding protein (RCP) which lacks 15 residues from the N-terminus and approximately 150 residues from the C-terminus.By similarity

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Structurei

    Secondary structure

    1
    317
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 84
    Helixi20 – 289
    Helixi33 – 5018
    Helixi58 – 7316
    Helixi76 – 8813
    Helixi93 – 997
    Helixi103 – 11816
    Helixi133 – 14412
    Helixi147 – 15913
    Helixi184 – 1863
    Helixi198 – 20811
    Helixi212 – 2165
    Beta strandi219 – 2268
    Beta strandi233 – 2353
    Helixi236 – 24611
    Beta strandi251 – 26111
    Helixi263 – 2653
    Beta strandi267 – 27610
    Turni278 – 2803
    Helixi281 – 2833
    Beta strandi286 – 2949
    Beta strandi300 – 31011
    Helixi311 – 3155

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M98X-ray2.10A/B1-317[»]
    ProteinModelPortaliP83689.
    SMRiP83689. Positions 2-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP83689.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni37 – 448Carotenoid-binding1 Publication
    Regioni107 – 1148Carotenoid-binding1 Publication
    Regioni151 – 1588Carotenoid-binding1 Publication
    Regioni203 – 2075Carotenoid-binding1 Publication
    Regioni247 – 2526Carotenoid-binding1 Publication
    Regioni275 – 2806Carotenoid-binding1 Publication

    Family and domain databases

    Gene3Di1.10.2090.10. 1 hit.
    InterProiIPR002075. NTF2.
    IPR015233. Orange_carotenoid-bd_N.
    [Graphical view]
    PfamiPF09150. Carot_N. 1 hit.
    PF02136. NTF2. 1 hit.
    [Graphical view]
    SUPFAMiSSF81930. SSF81930. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P83689-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPFTIDTARS IFPETLAADV VPATIARFKQ LSAEDQLALI WFAYLEMGKT    50
    ITIAAPGAAN MQFAENTLQE IRQMTPLQQT QAMCDLANRT DTPICRTYAS 100
    WSPNIKLGFW YELGRFMDQG LVAPIPEGYK LSANANAILV TIQGIDPGQQ 150
    ITVLRNCVVD MGFDTSKLGS YQRVAEPVVP PQEMSQRTKV QIEGVTNSTV 200
    LQYMDNLNAN DFDNLISLFA EDGALQPPFQ KPIVGKENTL RFFREECQNL 250
    KLIPERGVSE PTEDGYTQIK VTGKVQTPWF GGNVGMNIAW RFLLNPENKV 300
    FFVAIDLLAS PKELLNL 317
    Length:317
    Mass (Da):35,348
    Last modified:December 15, 2003 - v1
    Checksum:iD72A95D89DEF52EA
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M98 X-ray 2.10 A/B 1-317 [» ]
    ProteinModelPortali P83689.
    SMRi P83689. Positions 2-317.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P83689.

    Family and domain databases

    Gene3Di 1.10.2090.10. 1 hit.
    InterProi IPR002075. NTF2.
    IPR015233. Orange_carotenoid-bd_N.
    [Graphical view ]
    Pfami PF09150. Carot_N. 1 hit.
    PF02136. NTF2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81930. SSF81930. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The orange carotenoid protein of Synechocystis PCC 6803."
      Wu Y.P., Krogmann D.W.
      Biochim. Biophys. Acta 1322:1-7(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-29.
    2. "A carotenoid-protein from cyanobacteria."
      Holt T.K., Krogmann D.W.
      Biochim. Biophys. Acta 637:408-414(1981)
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, IDENTIFICATION OF CAROTENOID, PROTEOLYTIC CLEAVAGE.
    3. "Water-soluble carotenoid proteins of cyanobacteria."
      Kerfeld C.A.
      Arch. Biochem. Biophys. 430:2-9(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, PROTEOLYTIC CLEAVAGE.
    4. "Spectroscopic properties of the carotenoid 3'-hydroxyechinenone in the orange carotenoid protein from the cyanobacterium Arthrospira maxima."
      Polivka T., Kerfeld C.A., Pascher T., Sundstroem V.
      Biochemistry 44:3994-4003(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Crystals of the carotenoid protein from Arthrospira maxima containing uniformly oriented pigment molecules."
      Kerfeld C.A., Wu Y.P., Chan C., Krogmann D.W., Yeates T.O.
      Acta Crystallogr. D 53:720-723(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
    6. "The crystal structure of a cyanobacterial water-soluble carotenoid binding protein."
      Kerfeld C.A., Sawaya M.R., Brahmandam V., Cascio D., Ho K.K., Trevithick-Sutton C.C., Krogmann D.W., Yeates T.O.
      Structure 11:55-65(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH CAROTENOID, SUBUNIT.

    Entry informationi

    Entry nameiOCP_ARTMA
    AccessioniPrimary (citable) accession number: P83689
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Binds 1 carotenoid (3'-hydroxyechinenone) molecule and 1 chloride ion per subunit.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3