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P83689 (OCP_ARTMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Orange carotenoid-binding protein

Short name=OCP

Cleaved into the following chain:

  1. Red carotenoid-binding protein
    Short name=RCP
OrganismArthrospira maxima (Spirulina maxima)
Taxonomic identifier129910 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesArthrospira

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy. Ref.4 Ref.6

Subunit structure

Homodimer. Ref.3 Ref.6

Subcellular location

Cellular thylakoid membrane. Note: Associated with the phycobilisome By similarity. Ref.3 UniProtKB P74102

Post-translational modification

Proteolytically cleaved into a red 16.7 kDa form named red carotenoid-binding protein (RCP) which lacks 15 residues from the N-terminus and approximately 150 residues from the C-terminus By similarity. Ref.2 Ref.3

Miscellaneous

Binds 1 carotenoid (3'-hydroxyechinenone) molecule and 1 chloride ion per subunit.

Biophysicochemical properties

Absorption:

Abs(max)=494 nm Ref.2 Ref.3

Also shows other maxima at 465 nm and 275 nm. The red form (RCP) shows a broad peak at 500 nm. Ref.2

Ontologies

Keywords
   Cellular componentMembrane
Phycobilisome
Thylakoid
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processlight absorption

Inferred from electronic annotation. Source: InterPro

transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentphycobilisome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionchloride ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 317316Orange carotenoid-binding protein Ref.1
PRO_0000282350
Chain16 – ?Red carotenoid-binding protein By similarityPRO_0000282351

Regions

Region37 – 448Carotenoid-binding Ref.6
Region107 – 1148Carotenoid-binding Ref.6
Region151 – 1588Carotenoid-binding Ref.6
Region203 – 2075Carotenoid-binding Ref.6
Region247 – 2526Carotenoid-binding Ref.6
Region275 – 2806Carotenoid-binding Ref.6

Sites

Binding site531Carotenoid Ref.6
Binding site2271Carotenoid Ref.6
Binding site2771Chloride Ref.6
Binding site2861Carotenoid Ref.6
Binding site2901Carotenoid Ref.6
Binding site3051Carotenoid Ref.6

Secondary structure

............................................ 317
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P83689 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: D72A95D89DEF52EA

FASTA31735,348
        10         20         30         40         50         60 
MPFTIDTARS IFPETLAADV VPATIARFKQ LSAEDQLALI WFAYLEMGKT ITIAAPGAAN 

        70         80         90        100        110        120 
MQFAENTLQE IRQMTPLQQT QAMCDLANRT DTPICRTYAS WSPNIKLGFW YELGRFMDQG 

       130        140        150        160        170        180 
LVAPIPEGYK LSANANAILV TIQGIDPGQQ ITVLRNCVVD MGFDTSKLGS YQRVAEPVVP 

       190        200        210        220        230        240 
PQEMSQRTKV QIEGVTNSTV LQYMDNLNAN DFDNLISLFA EDGALQPPFQ KPIVGKENTL 

       250        260        270        280        290        300 
RFFREECQNL KLIPERGVSE PTEDGYTQIK VTGKVQTPWF GGNVGMNIAW RFLLNPENKV 

       310 
FFVAIDLLAS PKELLNL 

« Hide

References

[1]"The orange carotenoid protein of Synechocystis PCC 6803."
Wu Y.P., Krogmann D.W.
Biochim. Biophys. Acta 1322:1-7(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-29.
[2]"A carotenoid-protein from cyanobacteria."
Holt T.K., Krogmann D.W.
Biochim. Biophys. Acta 637:408-414(1981)
Cited for: BIOPHYSICOCHEMICAL PROPERTIES, IDENTIFICATION OF CAROTENOID, PROTEOLYTIC CLEAVAGE.
[3]"Water-soluble carotenoid proteins of cyanobacteria."
Kerfeld C.A.
Arch. Biochem. Biophys. 430:2-9(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, PROTEOLYTIC CLEAVAGE.
[4]"Spectroscopic properties of the carotenoid 3'-hydroxyechinenone in the orange carotenoid protein from the cyanobacterium Arthrospira maxima."
Polivka T., Kerfeld C.A., Pascher T., Sundstroem V.
Biochemistry 44:3994-4003(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Crystals of the carotenoid protein from Arthrospira maxima containing uniformly oriented pigment molecules."
Kerfeld C.A., Wu Y.P., Chan C., Krogmann D.W., Yeates T.O.
Acta Crystallogr. D 53:720-723(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION.
[6]"The crystal structure of a cyanobacterial water-soluble carotenoid binding protein."
Kerfeld C.A., Sawaya M.R., Brahmandam V., Cascio D., Ho K.K., Trevithick-Sutton C.C., Krogmann D.W., Yeates T.O.
Structure 11:55-65(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH CAROTENOID, SUBUNIT.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M98X-ray2.10A/B1-317[»]
ProteinModelPortalP83689.
SMRP83689. Positions 2-317.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.2090.10. 1 hit.
InterProIPR002075. NTF2.
IPR015233. Orange_carotenoid-bd_N.
[Graphical view]
PfamPF09150. Carot_N. 1 hit.
PF02136. NTF2. 1 hit.
[Graphical view]
SUPFAMSSF81930. SSF81930. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP83689.

Entry information

Entry nameOCP_ARTMA
AccessionPrimary (citable) accession number: P83689
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: December 15, 2003
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references