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P83673 (LYS1_CRAVI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 3, 2012. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysozyme 1

EC=3.2.1.17
Alternative name(s):
1,4-beta-N-acetylmuramidase 1
cv-lysozyme 1
Gene names
Name:lysoz1
Synonyms:lysoz
OrganismCrassostrea virginica (Eastern oyster)
Taxonomic identifier6565 [NCBI]
Taxonomic lineageEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaOstreoidaOstreoideaOstreidaeCrassostrea

Protein attributes

Sequence length184 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has antibacterial activity against the Gram-positive bacteria L.garvieae, M.luteus and Enterococcus sp., and the Gram-negative bacteria E.coli and V.vulnificus. Weak antibacterial activity against the Gram-negative bacterium A.hydrophila. No antibacterial activity detected against the Gram-positive bacterium S.iniae or against the Gram-negative bacterium E.ictaluri. Shows some chitinase activity but no isopeptidase activity. Ref.2 Ref.3

Catalytic activity

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Ref.2

Subcellular location

Secreted Ref.2.

Tissue specificity

Hemolymph, labial palps, non-vesiculated cells of mantle connective tissue, cells of interlamellar junctions and epithelia surrounding the water tubes of the gills. Ref.3

Sequence similarities

Belongs to the lysozyme type I family.

Biophysicochemical properties

pH dependence:

Optimum pH is 5.5-6.0. Ref.2

Temperature dependence:

Activity increases as temperature increases from 0 to 45 degrees Celsius and decreases markedly at temperatures greater than 55 degrees Celsius. Ref.2

Mass spectrometry

Molecular mass is 17771 Da from positions 21 - 184. Determined by MALDI. Ref.2

Molecular mass is 17861 Da from positions 21 - 184. Determined by MALDI. Ref.2

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionAntibiotic
Antimicrobial
Bacteriolytic enzyme
Glycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcytolysis

Inferred from electronic annotation. Source: UniProtKB-KW

defense response to Gram-negative bacterium

Inferred from direct assay Ref.2. Source: UniProtKB

defense response to Gram-positive bacterium

Inferred from direct assay Ref.2. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from direct assay Ref.2. Source: UniProtKB

   Molecular_functionlysozyme activity

Inferred from direct assay Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.2
Chain21 – 184164Lysozyme 1 Ref.2
PRO_0000084532

Experimental info

Sequence conflict431Y → T AA sequence Ref.2
Sequence conflict751Q → K AA sequence Ref.2
Sequence conflict1221Q → K AA sequence Ref.2
Sequence conflict1281L → I AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P83673 [UniParc].

Last modified March 20, 2007. Version 3.
Checksum: 239FC8C991546523

FASTA18419,986
        10         20         30         40         50         60 
MNGLILFCAV VFATAVCTYG SDAPCLRAGG RCQHDSITCS GRYRTGLCSG GVRRRCCVPS 

        70         80         90        100        110        120 
SSNSGSFSTG MVSQQCLRCI CNVESGCRPI GCHWDVNSDS CGYFQIKRAY WIDCGSPGGD 

       130        140        150        160        170        180 
WQTCANNLAC SSRCVQAYMA RYHRRSGCSN SCESFARIHN GGPRGCRNSN TEGYWRRVQA 


QGCN 

« Hide

References

[1]"Lysozyme gene in the eastern oyster, Crassostrea virginica."
Itoh N., Xue Q.-G., Cooper R.K., La Peyre J.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea virginica)."
Xue Q.-G., Schey K.L., Volety A.K., Chu F.-L., La Peyre J.F.
Comp. Biochem. Physiol. 139B:11-25(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-85; 109-141; 146-157 AND 168-176, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
Tissue: Hemolymph.
[3]"A new lysozyme from the eastern oyster (Crassostrea virginica) indicates adaptive evolution of i-type lysozymes."
Xue Q.-G., Itoh N., Schey K.L., Li Y.-L., Cooper R.K., La Peyre J.F.
Cell. Mol. Life Sci. 64:82-95(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB206328 mRNA. Translation: BAE47520.1.

3D structure databases

ProteinModelPortalP83673.
SMRP83673. Positions 67-179.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH22. Glycoside Hydrolase Family 22.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008597. Destabilase.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PANTHERPTHR11195. PTHR11195. 1 hit.
PfamPF05497. Destabilase. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLYS1_CRAVI
AccessionPrimary (citable) accession number: P83673
Secondary accession number(s): Q33DU2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 20, 2007
Last modified: October 3, 2012
This is version 39 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries