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P83673

- LYS1_CRAVI

UniProt

P83673 - LYS1_CRAVI

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Protein

Lysozyme 1

Gene

lysoz1

Organism
Crassostrea virginica (Eastern oyster)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has antibacterial activity against the Gram-positive bacteria L.garvieae, M.luteus and Enterococcus sp., and the Gram-negative bacteria E.coli and V.vulnificus. Weak antibacterial activity against the Gram-negative bacterium A.hydrophila. No antibacterial activity detected against the Gram-positive bacterium S.iniae or against the Gram-negative bacterium E.ictaluri. Shows some chitinase activity but no isopeptidase activity.2 Publications

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

pH dependencei

Optimum pH is 5.5-6.0.1 Publication

Temperature dependencei

Activity increases as temperature increases from 0 to 45 degrees Celsius and decreases markedly at temperatures greater than 55 degrees Celsius.1 Publication

GO - Molecular functioni

  1. lysozyme activity Source: UniProtKB

GO - Biological processi

  1. cytolysis Source: UniProtKB-KW
  2. defense response to Gram-negative bacterium Source: UniProtKB
  3. defense response to Gram-positive bacterium Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH22. Glycoside Hydrolase Family 22.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysozyme 1 (EC:3.2.1.17)
Alternative name(s):
1,4-beta-N-acetylmuramidase 1
cv-lysozyme 1
Gene namesi
Name:lysoz11 Publication
Synonyms:lysozImported
OrganismiCrassostrea virginica (Eastern oyster)
Taxonomic identifieri6565 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaOstreoidaOstreoideaOstreidaeCrassostrea

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 PublicationAdd
BLAST
Chaini21 – 184164Lysozyme 11 PublicationPRO_0000084532Add
BLAST

Expressioni

Tissue specificityi

Hemolymph, labial palps, non-vesiculated cells of mantle connective tissue, cells of interlamellar junctions and epithelia surrounding the water tubes of the gills.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP83673.
SMRiP83673. Positions 67-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the lysozyme type I family.Sequence Analysis

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR008597. Destabilase.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PANTHERiPTHR11195. PTHR11195. 1 hit.
PfamiPF05497. Destabilase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83673-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGLILFCAV VFATAVCTYG SDAPCLRAGG RCQHDSITCS GRYRTGLCSG
60 70 80 90 100
GVRRRCCVPS SSNSGSFSTG MVSQQCLRCI CNVESGCRPI GCHWDVNSDS
110 120 130 140 150
CGYFQIKRAY WIDCGSPGGD WQTCANNLAC SSRCVQAYMA RYHRRSGCSN
160 170 180
SCESFARIHN GGPRGCRNSN TEGYWRRVQA QGCN
Length:184
Mass (Da):19,986
Last modified:March 20, 2007 - v3
Checksum:i239FC8C991546523
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431Y → T AA sequence (PubMed:15364284)Curated
Sequence conflicti75 – 751Q → K AA sequence (PubMed:15364284)Curated
Sequence conflicti122 – 1221Q → K AA sequence (PubMed:15364284)Curated
Sequence conflicti128 – 1281L → I AA sequence (PubMed:15364284)Curated

Mass spectrometryi

Molecular mass is 17771 Da from positions 21 - 184. Determined by MALDI. 1 Publication
Molecular mass is 17861 Da from positions 21 - 184. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB206328 mRNA. Translation: BAE47520.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB206328 mRNA. Translation: BAE47520.1 .

3D structure databases

ProteinModelPortali P83673.
SMRi P83673. Positions 67-179.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH22. Glycoside Hydrolase Family 22.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008597. Destabilase.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
PANTHERi PTHR11195. PTHR11195. 1 hit.
Pfami PF05497. Destabilase. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Lysozyme gene in the eastern oyster, Crassostrea virginica."
    Itoh N., Xue Q.-G., Cooper R.K., La Peyre J.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea virginica)."
    Xue Q.-G., Schey K.L., Volety A.K., Chu F.-L., La Peyre J.F.
    Comp. Biochem. Physiol. 139B:11-25(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-85; 109-141; 146-157 AND 168-176, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
    Tissue: Hemolymph1 Publication.
  3. "A new lysozyme from the eastern oyster (Crassostrea virginica) indicates adaptive evolution of i-type lysozymes."
    Xue Q.-G., Itoh N., Schey K.L., Li Y.-L., Cooper R.K., La Peyre J.F.
    Cell. Mol. Life Sci. 64:82-95(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLYS1_CRAVI
AccessioniPrimary (citable) accession number: P83673
Secondary accession number(s): Q33DU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 20, 2007
Last modified: October 1, 2014
This is version 40 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3