Reviewed, UniProtKB/Swiss-Prot P83673 (LYS1_CRAVI)

Names and origin

Protein names

Recommended name:
    Lysozyme 1
    EC=3.2.1.17
Alternative name(s):
    1,4-beta-N-acetylmuramidase 1
    cv-lysozyme 1

Gene names

Name:	lysoz1
Synonyms:	lysoz

Organism

Crassostrea virginica (Eastern oyster)

Taxonomic identifier

6565 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Mollusca › Bivalvia › Pteriomorphia › Ostreoida › Ostreoidea › Ostreidae › Crassostrea

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has antibacterial activity against the Gram-positive bacteria L.garvieae, M.luteus and Enterococcus sp., and the Gram-negative bacteria E.coli and V.vulnificus. Weak antibacterial activity against the Gram-negative bacterium A.hydrophila. No antibacterial activity detected against the Gram-positive bacterium S.iniae or against the Gram-negative bacterium E.ictaluri. Shows some chitinase activity but no isopeptidase activity. Ref.2 Ref.3
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Ref.2
Subcellular location	Secreted Ref.2.
Tissue specificity	Hemolymph, labial palps, non-vesiculated cells of mantle connective tissue, cells of interlamellar junctions and epithelia surrounding the water tubes of the gills. Ref.3
Sequence similarities	Belongs to the lysozyme type I family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.5-6.0. Ref.2 Temperature dependence: Activity increases as temperature increases from 0 to 45 degrees Celsius and decreases markedly at temperatures greater than 55 degrees Celsius. Ref.2
Mass spectrometry	Molecular mass is 17771 Da from positions 21 - 184. Determined by MALDI. Ref.2 Molecular mass is 17861 Da from positions 21 - 184. Determined by MALDI. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to Gram-negative bacterium Ref.2 Inferred from direct assay. Source: UniProtKB defense response to Gram-positive bacterium Ref.2 Inferred from direct assay. Source: UniProtKB metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Ref.2 Inferred from direct assay. Source: UniProtKB
Molecular function	lysozyme activity Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.2
	Chain	21 – 184	164	Lysozyme 1 Ref.2		PRO_0000084532
Experimental info
	Sequence conflict	43	1	Y → T AA sequence Ref.2
	Sequence conflict	75	1	Q → K AA sequence Ref.2
	Sequence conflict	122	1	Q → K AA sequence Ref.2
	Sequence conflict	128	1	L → I AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P83673-1 [UniParc]. Last modified March 20, 2007. Version 3. Checksum: 239FC8C991546523	FASTA	184	19,986
	10 20 30 40 50 60 MNGLILFCAV VFATAVCTYG SDAPCLRAGG RCQHDSITCS GRYRTGLCSG GVRRRCCVPS 70 80 90 100 110 120 SSNSGSFSTG MVSQQCLRCI CNVESGCRPI GCHWDVNSDS CGYFQIKRAY WIDCGSPGGD 130 140 150 160 170 180 WQTCANNLAC SSRCVQAYMA RYHRRSGCSN SCESFARIHN GGPRGCRNSN TEGYWRRVQA QGCN

References

[1]	"Lysozyme gene in the eastern oyster, Crassostrea virginica." Itoh N., Xue Q.-G., Cooper R.K., La Peyre J.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea virginica)." Xue Q.-G., Schey K.L., Volety A.K., Chu F.-L., La Peyre J.F. Comp. Biochem. Physiol. 139B:11-25(2004) [PubMed: 15364284] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-85; 109-141; 146-157 AND 168-176, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MASS SPECTROMETRY. Tissue: Hemolymph.
[3]	"A new lysozyme from the eastern oyster (Crassostrea virginica) indicates adaptive evolution of i-type lysozymes." Xue Q.-G., Itoh N., Schey K.L., Li Y.-L., Cooper R.K., La Peyre J.F. Cell. Mol. Life Sci. 64:82-95(2007) [PubMed: 17160350] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB206328 mRNA. Translation: BAE47520.1.
3D structure databases
SMR	P83673. Positions 67-179.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Enzyme and pathway databases
BRENDA	3.2.1.17. 141032.
Family and domain databases
InterPro	IPR008597. Destabilase. [Graphical view]
PANTHER	PTHR11195. Destabilase. 1 hit.
Pfam	PF05497. Destabilase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS1_CRAVI

Accession

Primary (citable) accession number: P83673
Secondary accession number(s): Q33DU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 20, 2007
Last modified:	January 19, 2010
This is version 34 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)