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P83673

- LYS1_CRAVI

UniProt

P83673 - LYS1_CRAVI

Protein

Lysozyme 1

Gene

lysoz1

Organism
Crassostrea virginica (Eastern oyster)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 3 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Has antibacterial activity against the Gram-positive bacteria L.garvieae, M.luteus and Enterococcus sp., and the Gram-negative bacteria E.coli and V.vulnificus. Weak antibacterial activity against the Gram-negative bacterium A.hydrophila. No antibacterial activity detected against the Gram-positive bacterium S.iniae or against the Gram-negative bacterium E.ictaluri. Shows some chitinase activity but no isopeptidase activity.2 Publications

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

    pH dependencei

    Optimum pH is 5.5-6.0.1 Publication

    Temperature dependencei

    Activity increases as temperature increases from 0 to 45 degrees Celsius and decreases markedly at temperatures greater than 55 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. lysozyme activity Source: UniProtKB

    GO - Biological processi

    1. cytolysis Source: UniProtKB-KW
    2. defense response to Gram-negative bacterium Source: UniProtKB
    3. defense response to Gram-positive bacterium Source: UniProtKB

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH22. Glycoside Hydrolase Family 22.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysozyme 1 (EC:3.2.1.17)
    Alternative name(s):
    1,4-beta-N-acetylmuramidase 1
    cv-lysozyme 1
    Gene namesi
    Name:lysoz11 Publication
    Synonyms:lysozImported
    OrganismiCrassostrea virginica (Eastern oyster)
    Taxonomic identifieri6565 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaOstreoidaOstreoideaOstreidaeCrassostrea

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 20201 PublicationAdd
    BLAST
    Chaini21 – 184164Lysozyme 11 PublicationPRO_0000084532Add
    BLAST

    Expressioni

    Tissue specificityi

    Hemolymph, labial palps, non-vesiculated cells of mantle connective tissue, cells of interlamellar junctions and epithelia surrounding the water tubes of the gills.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP83673.
    SMRiP83673. Positions 67-179.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the lysozyme type I family.Sequence Analysis

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR008597. Destabilase.
    IPR023346. Lysozyme-like_dom.
    [Graphical view]
    PANTHERiPTHR11195. PTHR11195. 1 hit.
    PfamiPF05497. Destabilase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P83673-1 [UniParc]FASTAAdd to Basket

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    MNGLILFCAV VFATAVCTYG SDAPCLRAGG RCQHDSITCS GRYRTGLCSG    50
    GVRRRCCVPS SSNSGSFSTG MVSQQCLRCI CNVESGCRPI GCHWDVNSDS 100
    CGYFQIKRAY WIDCGSPGGD WQTCANNLAC SSRCVQAYMA RYHRRSGCSN 150
    SCESFARIHN GGPRGCRNSN TEGYWRRVQA QGCN 184
    Length:184
    Mass (Da):19,986
    Last modified:March 20, 2007 - v3
    Checksum:i239FC8C991546523
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti43 – 431Y → T AA sequence (PubMed:15364284)Curated
    Sequence conflicti75 – 751Q → K AA sequence (PubMed:15364284)Curated
    Sequence conflicti122 – 1221Q → K AA sequence (PubMed:15364284)Curated
    Sequence conflicti128 – 1281L → I AA sequence (PubMed:15364284)Curated

    Mass spectrometryi

    Molecular mass is 17771 Da from positions 21 - 184. Determined by MALDI. 1 Publication
    Molecular mass is 17861 Da from positions 21 - 184. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB206328 mRNA. Translation: BAE47520.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB206328 mRNA. Translation: BAE47520.1 .

    3D structure databases

    ProteinModelPortali P83673.
    SMRi P83673. Positions 67-179.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH22. Glycoside Hydrolase Family 22.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008597. Destabilase.
    IPR023346. Lysozyme-like_dom.
    [Graphical view ]
    PANTHERi PTHR11195. PTHR11195. 1 hit.
    Pfami PF05497. Destabilase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Lysozyme gene in the eastern oyster, Crassostrea virginica."
      Itoh N., Xue Q.-G., Cooper R.K., La Peyre J.F.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea virginica)."
      Xue Q.-G., Schey K.L., Volety A.K., Chu F.-L., La Peyre J.F.
      Comp. Biochem. Physiol. 139B:11-25(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-85; 109-141; 146-157 AND 168-176, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
      Tissue: Hemolymph1 Publication.
    3. "A new lysozyme from the eastern oyster (Crassostrea virginica) indicates adaptive evolution of i-type lysozymes."
      Xue Q.-G., Itoh N., Schey K.L., Li Y.-L., Cooper R.K., La Peyre J.F.
      Cell. Mol. Life Sci. 64:82-95(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiLYS1_CRAVI
    AccessioniPrimary (citable) accession number: P83673
    Secondary accession number(s): Q33DU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 40 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3