Reviewed, UniProtKB/Swiss-Prot P83658 (DISS_ECHCA)

Names and origin

Protein names	Recommended name:     Disintegrin schistatin
Organism	Echis carinatus (Saw-scaled viper)
Taxonomic identifier	40353 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Viperinae › Echis

Protein attributes

Sequence length	64 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May bind with both glycoprotein IIb-IIIa (ITGA2B/ITGB3) and alphavbeta3 (ITGAV/ITGB3) integrins, and may inhibit platelet aggregation.
Subunit structure	Homodimer; disulfide-linked. Ref.1 Ref.3
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Sequence similarities	Belongs to the disintegrin family. Contains 1 disintegrin domain.

Ontologies

Keywords
Biological process	Blood coagulation Cell adhesion
Cellular component	Secreted
Molecular function	Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 64

64

Disintegrin schistatin

PRO_0000101800

Regions

Domain

1 – 64

64

Disintegrin

Motif

42 – 44

3

Cell attachment site By similarity UniProtKB P81631

Amino acid modifications

Disulfide bond

6 ↔ 29

Ref.1 Ref.3

Disulfide bond

7

Interchain (with C-12) Ref.1 Ref.3

Disulfide bond

12

Interchain (with C-7) Ref.1 Ref.3

Disulfide bond

20 ↔ 26

Ref.1 Ref.3

Disulfide bond

25 ↔ 50

Ref.1 Ref.3

Disulfide bond

38 ↔ 57

Ref.1 Ref.3

Experimental info

Sequence conflict

8

1

D → P AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

64

Helix Strand Turn

Details...

Turn

9 – 12

4

Beta strand

21 – 23

3

Beta strand

37 – 39

3

Beta strand

42 – 45

4

Sequences

	Sequence		Length	Mass (Da)
	P83658-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: FD0CA30A2048EE51	FASTA	64	7,083
	10 20 30 40 50 60 NSVHPCCDPV ICEPREGEHC ISGPCCENCY FLNSGTICKR ARGDGNQDYC TGITPDCPRN RYNV

References

[1]	Bilgrami S., Jabeen T., Kumar J., Yadav S., Sharma S., Kaur P., Singh T.P. Submitted (SEP-2003) to UniProtKB Cited for: PROTEIN SEQUENCE, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISULFIDE BONDS. Tissue: Venom.
[2]	"Purification, crystallization and preliminary X-ray diffraction studies of disintegrin (schistatin) from saw-scaled viper (Echis carinatus)." Tomar S., Yadav S., Chandra V., Kumar P., Singh T.P. Acta Crystallogr. D 57:1669-1670(2001) [PubMed: 11679739] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-19, X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). Tissue: Venom.
[3]	"Crystal structure of schistatin, a disintegrin homodimer from saw-scaled viper (Echis carinatus) at 2.5 A resolution." Bilgrami S., Tomar S., Yadav S., Kaur P., Kumar J., Jabeen T., Sharma S., Singh T.P. J. Mol. Biol. 341:829-837(2004) [PubMed: 15317139] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RMR	X-ray	2.50	A	1-64	[»]
1TEJ	X-ray	1.90	A/B	1-64	[»]

ModBase

Search...

Phylogenomic databases

HOVERGEN

P83658.

Family and domain databases

InterPro

IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
[Graphical view]

Gene3D

G3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.

Pfam

PF00200. Disintegrin. 1 hit.
[Graphical view]

PRINTS

PR00289. DISINTEGRIN.

SMART

SM00050. DISIN. 1 hit.
[Graphical view]

PROSITE

PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DISS_ECHCA

Accession

Primary (citable) accession number: P83658

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2003
Last modified:	November 24, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)