Reviewed, UniProtKB/Swiss-Prot P83658 (DISS_ECHCA)

Names and origin

Protein names	Recommended name:     Disintegrin schistatin
Organism	Echis carinatus (Saw-scaled viper)
Taxonomic identifier	40353 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Viperinae › Echis

Protein attributes

Sequence length	64 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May bind with both glycoprotein IIb-IIIa (ITGA2B/ITGB3) and alphavbeta3 (ITGAV/ITGB3) integrins, and may inhibit platelet aggregation.
Subunit structure	Homodimer; disulfide-linked. Ref.1 Ref.3
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Sequence similarities	Belongs to the disintegrin family. Contains 1 disintegrin domain.

Ontologies

Keywords
Biological process	Blood coagulation Cell adhesion
Cellular component	Secreted
Molecular function	Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 64

64

Disintegrin schistatin

PRO_0000101800

Regions

Domain

1 – 64

64

Disintegrin

Motif

42 – 44

3

Cell attachment site By similarity UniProtKB P81631

Amino acid modifications

Disulfide bond

6 ↔ 29

Ref.1 Ref.3

Disulfide bond

7

Interchain (with C-12) Ref.1 Ref.3

Disulfide bond

12

Interchain (with C-7) Ref.1 Ref.3

Disulfide bond

20 ↔ 26

Ref.1 Ref.3

Disulfide bond

25 ↔ 50

Ref.1 Ref.3

Disulfide bond

38 ↔ 57

Ref.1 Ref.3

Experimental info

Sequence conflict

8

1

D → P AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

64

Helix Strand Turn

Details...

Turn

9 – 12

4

Beta strand

21 – 23

3

Beta strand

37 – 39

3

Beta strand

42 – 45

4

Sequences

	Sequence		Length	Mass (Da)
	P83658-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: FD0CA30A2048EE51	FASTA	64	7,083
	10 20 30 40 50 60 NSVHPCCDPV ICEPREGEHC ISGPCCENCY FLNSGTICKR ARGDGNQDYC TGITPDCPRN RYNV

References

[1]	Bilgrami S., Jabeen T., Kumar J., Yadav S., Sharma S., Kaur P., Singh T.P. Submitted (SEP-2003) to UniProtKB Cited for: PROTEIN SEQUENCE, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISULFIDE BONDS. Tissue: Venom.
[2]	"Purification, crystallization and preliminary X-ray diffraction studies of disintegrin (schistatin) from saw-scaled viper (Echis carinatus)." Tomar S., Yadav S., Chandra V., Kumar P., Singh T.P. Acta Crystallogr. D 57:1669-1670(2001) [PubMed: 11679739] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-19, X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). Tissue: Venom.
[3]	"Crystal structure of schistatin, a disintegrin homodimer from saw-scaled viper (Echis carinatus) at 2.5 A resolution." Bilgrami S., Tomar S., Yadav S., Kaur P., Kumar J., Jabeen T., Sharma S., Singh T.P. J. Mol. Biol. 341:829-837(2004) [PubMed: 15317139] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RMR	X-ray	2.50	A	1-64	[»]
1TEJ	X-ray	1.90	A/B	1-64	[»]

ModBase

Search...

Phylogenomic databases

HOVERGEN

P83658.

Family and domain databases

InterPro

IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
[Graphical view]

Gene3D

G3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.

Pfam

PF00200. Disintegrin. 1 hit.
[Graphical view]

PRINTS

PR00289. DISINTEGRIN.

SMART

SM00050. DISIN. 1 hit.
[Graphical view]

PROSITE

PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DISS_ECHCA

Accession

Primary (citable) accession number: P83658

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2003
Last modified:	November 24, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)