Reviewed, UniProtKB/Swiss-Prot P83645 (GPX2_RAT)

Names and origin

Protein names

Recommended name:
    Glutathione peroxidase 2
      Short name=GSHPx-2
      Short name=GPx-2
    EC=1.11.1.9
Alternative name(s):
    Glutathione peroxidase-gastrointestinal
      Short name=GSHPx-GI
      Short name=GPx-GI

Gene names

Name:

Gpx2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 glutathione + H2O2 = glutathione disulfide + 2 H2O. Ref.3
Subunit structure	Homotetramer By similarity. UniProtKB P18283
Subcellular location	Cytoplasm By similarity UniProtKB P18283.
Tissue specificity	Mucosal epithelium of the gastrointestinal tract. Ref.3
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Selenocysteine
Ligand	Selenium
Molecular function	Oxidoreductase Peroxidase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Ref.3 Inferred from direct assay. Source: RGD selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Glutathione peroxidase 2		PRO_0000066624
Sites
	Active site	40	1	By similarity
Amino acid modifications
	Non-standard residue	40	1	Selenocysteine
Experimental info
	Sequence conflict	91	1	H → P AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P83645-1 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 9A69384CBEF81E70	FASTA	190	22,014
	10 20 30 40 50 60 MAYIAKSFYD LSAIGLDGEK IDFNTFRGRA VLIENVASLU GTTTRDYTQL NELQCRFPRR 70 80 90 100 110 120 LVVLGFPCNQ FGHQENCQNE EILNSLKYVR HGGGFQPTFS LTQKCDVNGQ NQHPVFAYLK 130 140 150 160 170 180 DKLPYPYDDP FSLMTDPKLI IWSPVRRSDV SWNFEKFLIG PEGEPFRRYS RTFQTINIEP 190 DIKRLLKVAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distinct gene expression profiles of rat dorsal root ganglion induced by peripheral nerve axotomy." Xiao H.S., Han Z.G., Zhang F.X., Huang Q.H., Lu Y.J., Bao L., Fu G., Guo C., Yan Q., Jin S.X., Zhu Z.D., Xu X.R., Li N.G., Chen Z., Zhang X. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-87. Strain: Sprague-Dawley. Tissue: Spinal ganglion.
[2]	"Normalization and subtraction: two approaches to facilitate gene discovery." Bonaldo M.F., Lennon G., Soares M.B. Genome Res. 6:791-806(1996) [PubMed: 8889548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 80-190. Strain: Sprague-Dawley. Tissue: Ileum.
[3]	"Selenium-dependent glutathione peroxidase-GI is a major glutathione peroxidase activity in the mucosal epithelium of rodent intestine." Esworthy R.S., Swiderek K.M., Ho Y.-S., Chu F.-F. Biochim. Biophys. Acta 1381:213-226(1998) [PubMed: 9685647] [Abstract] Cited for: PROTEIN SEQUENCE OF 7-27; 91-101; 121-138; 140-168 AND 175-181, CATALYTIC ACTIVITY, TISSUE SPECIFICITY. Strain: CD Charles River. Tissue: Gastrointestinal tract epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BG664050 mRNA. No translation available. BQ196649 mRNA. No translation available.
IPI	IPI00339191.
UniGene	Rn.3503
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P83645.
Protein family/group databases
PeroxiBase	3731. RnoGPx02.
Genome annotation databases
Ensembl	ENSRNOT00000058591; ENSRNOP00000055389; ENSRNOG00000038455; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	727780. Gpx2.
Phylogenomic databases
eggNOG	maNOG16607.
HOVERGEN	P83645.
InParanoid	P83645.
Enzyme and pathway databases
BRENDA	1.11.1.9. 248.
Gene expression databases
Genevestigator	P83645.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX2_RAT

Accession

Primary (citable) accession number: P83645

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)