P83597 (EAP2_EUCUL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Antifungal peptide 2 Alternative name(s): EAFP2
Organism	Eucommia ulmoides (Hardy rubber tree)
Taxonomic identifier	4392 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Garryales › Eucommiaceae › Eucommia

Protein attributes

Sequence length	41 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has antifungal activity against P.infestans, A.lycopersici, V.dahliae, G.zeae, A.nicotianae, F.moniliforme, F.oxysporum and C.gossypii. Ref.1
Subunit structure	Monomer. Ref.1
Sequence similarities	Contains 1 chitin-binding type-1 domain.
Mass spectrometry	Molecular mass is 4158.91 Da from positions 1 - 41. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Plant defense
Ligand	Chitin-binding
Molecular function	Antimicrobial Fungicide
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of other organism Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 41

41

Antifungal peptide 2

PRO_0000124817

Regions

Domain

4 – 41

38

Chitin-binding type-1

Amino acid modifications

Modified residue

1

1

Pyrrolidone carboxylic acid

Disulfide bond

3 ↔ 17

Ref.1 Ref.2 Ref.3

Disulfide bond

7 ↔ 37

Ref.1 Ref.2 Ref.3

Disulfide bond

11 ↔ 23

Ref.1 Ref.2 Ref.3

Disulfide bond

16 ↔ 30

Ref.1 Ref.2 Ref.3

Disulfide bond

35 ↔ 39

Ref.1 Ref.2 Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

41

Helix Strand Turn

Details...

Helix

3 – 6

4

Beta strand

16 – 18

3

Helix

27 – 30

4

Turn

32 – 34

3

Beta strand

35 – 37

3

Sequences

	Sequence		Length	Mass (Da)
	P83597 [UniParc]. Last modified June 27, 2003. Version 1. Checksum: 8F775334FAABA647	FASTA	41	4,186
	10 20 30 40 QTCASRCPRP CNAGLCCSIY GYCGSGAAYC GAGNCRCQCR G

References

[1]	"Two novel antifungal peptides distinct with a five-disulfide motif from the bark of Eucommia ulmoides Oliv." Huang R.-H., Xiang Y., Liu X.-Z., Zhang Y., Hu Z., Wang D.-C. FEBS Lett. 521:87-90(2002) [PubMed: 12067732] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT, MASS SPECTROMETRY, DISULFIDE BONDS, PYROGLUTAMATE FORMATION AT GLN-1. Tissue: Bark.
[2]	"Solution structure of Eucommia antifungal peptide: a novel structural model distinct with a five-disulfide motif." Huang R.H., Xiang Y., Tu G.Z., Zhang Y., Wang D.C. Biochemistry 43:6005-6012(2004) [PubMed: 15147184] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.
[3]	"Crystal structure of a novel antifungal protein distinct with five disulfide bridges from Eucommia ulmoides Oliver at an atomic resolution." Xiang Y., Huang R.H., Liu X.Z., Zhang Y., Wang D.C. J. Struct. Biol. 148:86-97(2004) [PubMed: 15363789] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.84 ANGSTROMS), DISULFIDE BONDS.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P9G	X-ray	0.84	A	2-41	[»]
1P9Z	NMR	-	A	2-41	[»]

ProteinModelPortal

P83597.

SMR

P83597. Positions 1-41.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
[Graphical view]

Gene3D

G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.

Pfam

PF00187. Chitin_bind_1. 1 hit.
[Graphical view]

SUPFAM

SSF57016. Chitin_bd_1. 1 hit.

PROSITE

PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

EAP2_EUCUL

Accession

Primary (citable) accession number: P83597

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	June 27, 2003
Last modified:	April 5, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program