P83581 (HS74L_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock 70 kDa protein 4L

Alternative name(s):
Heat shock 70-related protein APG-1
Osmotic stress protein 94

Gene names

Name:	Hspa4l
Synonyms:	Apg1, Hsp4l, Osp94

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	24 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possesses chaperone activity in vitro where it inhibits aggregation of citrate synthase. Ref.1
Subunit structure	Homodimer. In the testis, forms a complex with p53 at 32.5 degrees Celsius which is scrotal temperature but not at 37 or 42 degrees Celsius. Ref.2 Ref.4
Subcellular location	Cytoplasm. Nucleus. Note: May translocate to the nucleus of germ cells after heat shock. Ref.1 Ref.4
Tissue specificity	Expressed at high levels in testis and at much lower levels in brain. In testis, expressed mainly in germ cells. Widespread in brain with highest expression in cerebellum and medulla oblongata. Also expressed in renal medulla of water-restricted animals. Ref.1 Ref.2 Ref.4
Developmental stage	Expression is first detected in the testis 5 weeks after birth and coincides with the appearance of spermatozoa. In the brain, expression is first detected 3.5 days after birth. Ref.1 Ref.3
Induction	By heat shock. Ref.4
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from direct assay Ref.1. Source: UniProtKB response to unfolded protein Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB nucleus Inferred from direct assay Ref.4. Source: UniProtKB
Molecular_function	ATP binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›24	›24	Heat shock 70 kDa protein 4L		PRO_0000078282
Amino acid modifications
	Modified residue	19	1	Phosphothreonine By similarity
Experimental info
	Non-adjacent residues	15 – 16	2
	Non-terminal residue	24	1

Sequences

	Sequence		Length	Mass (Da)
	P83581 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: 62B092E031786E9F	FASTA	24	2,796
	10 20 LSAMLEDTEN WLYEELSLTQ DPVV

References

[1]	"Characterization of the 105-kDa molecular chaperone. Identification, biochemical properties, and localization." Matsumori M., Itoh H., Toyoshima I., Komatsuda A., Sawada K., Fukuda J., Tanaka T., Okubo A., Kinouchi H., Mizoi K., Hama T., Suzuki A., Hamada F., Otaka M., Shoji Y., Takada G. Eur. J. Biochem. 269:5632-5641(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Testis.
[2]	"A novel testis-specific 105-kDa protein related to the 90-kDa heat-shock protein." Itoh H., Tashima Y. Eur. J. Biochem. 193:429-435(1990) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, TISSUE SPECIFICITY.
[3]	"Different expression time of the 105-kDa protein and 90-kDa heat-shock protein in rat testis." Itoh H., Tashima Y. FEBS Lett. 289:110-112(1991) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[4]	"Germ cell-specific heat shock protein 105 binds to p53 in a temperature-sensitive manner in rat testis." Kumagai J., Fukuda J., Kodama H., Murata M., Kawamura K., Itoh H., Tanaka T. Eur. J. Biochem. 267:3073-3078(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, FORMATION OF COMPLEX WITH P53. Strain: Wistar. Tissue: Testis.

Cross-references

Sequence databases
IPI	IPI00337270.
UniGene	Rn.144829.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P83581.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:1306528. rat.
Organism-specific databases
RGD	1306528. Hspa4l.
Gene expression databases
Genevestigator	P83581.
Family and domain databases
PROSITE	PS00297. HSP70_1. Partial match. PS00329. HSP70_2. Partial match. PS01036. HSP70_3. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HS74L_RAT

Accession

Primary (citable) accession number: P83581

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program