Reviewed, UniProtKB/Swiss-Prot P83572 (ARALA_ARAEL)

Names and origin

Protein names	Recommended name:     Aralin A chain     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Aralia elata (Japanese angelica tree)
Taxonomic identifier	82095 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Araliaceae › Aralia

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Toxic type II ribosome-inactivating protein (RIP). Induces apoptosis. Has cytotoxic activity against several human cancer cell lines. Is less cytotoxic to normal human cells. Ref.1 Ref.3
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Subunit structure	Disulfide-linked dimer of A and B chains. Ref.1
Post-translational modification	Glycosylated. Ref.1
Miscellaneous	The inhibitory concentration 50% (IC50) is 0.8 ng/ml for transformed cells and 10 ng/ml for normal cells.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 2 RIP subfamily.

Ontologies

Keywords
Biological process	Apoptosis
Molecular function	Hydrolase Toxin
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis Ref.1 Inferred from direct assay. Source: UniProtKB pathogenesis Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	Aralin A chain		PRO_0000221396
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	P83572-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 857D34B58BFDB672	FASTA	25	2,734
	10 20 ANFPTVSLNT VGITRQIPQD FMNAV

References

[1]	"Aralin, a new cytotoxic protein from Aralia elata, inducing apoptosis in human cancer cells." Tomatsu M., Ohnishi-Kameyama M., Shibamoto N. Cancer Lett. 199:19-25(2003) [PubMed: 12963119] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT, GLYCOSYLATION. Tissue: Shoot.
[2]	"Production of aralin, a selective cytotoxic lectin against human transformed cells, in callus culture of Aralia elata." Tomatsu M., Mujin T., Shibamoto N., Tashiro F., Ikuta A. Planta Med. 70:469-471(2004) [PubMed: 15124096] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, CHARACTERIZATION.
[3]	"An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata." Tomatsu M., Kondo T., Yoshikawa T., Komeno T., Adachi N., Kawasaki Y., Ikuta A., Tashiro F. Biol. Chem. 385:819-827(2004) [PubMed: 15493877] [Abstract] Cited for: FUNCTION.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 278262.
Family and domain databases
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARALA_ARAEL

Accession

Primary (citable) accession number: P83572

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 15, 2003
Last modified:	November 24, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)