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Protein

RuvB-like 2

Gene

ruvbl2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has double-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity suggesting a role in nuclear processes such as recombination and transcription. Represses gene activation mediated by beta-catenin.By similarity1 Publication
Proposed core component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.By similarity
Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 848ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • axonemal dynein complex assembly Source: ZFIN
  • digestive tract development Source: ZFIN
  • DNA duplex unwinding Source: GOC
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • epithelial cilium movement Source: ZFIN
  • heart development Source: ZFIN
  • motile cilium assembly Source: ZFIN
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of heart growth Source: ZFIN
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RuvB-like 2 (EC:3.6.4.12)
Alternative name(s):
Reptin
zReptin
Gene namesi
Name:ruvbl2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 5

Organism-specific databases

ZFINiZDB-GENE-030109-1. ruvbl2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Fishes display embryonic cardiac hyperplasia with increased cell number and size by 72 hours post-fertilization. Gut and gut-derived organs are incapable of growth and differentiation after this time. The mutation enhances ATPase activity, rendering it DNA-independent and causes aggregation of the protein into large complexes.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi190 – 1901G → GFCR in LIK mutant; causes embryonic cardiac hyperplasia. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463RuvB-like 2PRO_0000165646Add
BLAST

Proteomic databases

PaxDbiP83571.
PRIDEiP83571.

Expressioni

Developmental stagei

Widely expressed in the embryo. After 60 hours post-fertilization there is increased expression in heart, liver, branchial arches, exocrine pancreas and intestine.1 Publication

Gene expression databases

BgeeiP83571.

Interactioni

Subunit structurei

Forms homohexameric rings (Probable). Can form a dodecamer with ruvbl1 made of two stacked hexameric rings. Component of the chromatin-remodeling Ino80 complex. Component of some MLL1/MLL complex (By similarity).By similarityCurated

Protein-protein interaction databases

STRINGi7955.ENSDARP00000072482.

Structurei

3D structure databases

ProteinModelPortaliP83571.
SMRiP83571. Positions 132-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.By similarity

Phylogenomic databases

eggNOGiKOG2680. Eukaryota.
COG1224. LUCA.
GeneTreeiENSGT00550000075034.
HOGENOMiHOG000190885.
HOVERGENiHBG054186.
InParanoidiP83571.
KOiK11338.
OMAiYDAMGAQ.
OrthoDBiEOG75TMBR.
PhylomeDBiP83571.
TreeFamiTF300469.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR027238. RuvB-like.
IPR010339. TIP49_C.
[Graphical view]
PANTHERiPTHR11093. PTHR11093. 1 hit.
PfamiPF06068. TIP49. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P83571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQVATTKV PEVRDITRIE RIGAHSHIRG LGLDDALEPR QVSQGMVGQL
60 70 80 90 100
ASRRAAGLIL EMIKDGQIAG RAVLIAGQPG TGKTAIAMGI AQSLGPDTPF
110 120 130 140 150
TALAGSEIFS LEMSKTEALS QAFRKAIGVR IKEETEIIEG EVVEIQIDRP
160 170 180 190 200
ATGTGAKVGK LTLKTTEMET IYDLGTKMIE SLSKERVQAG DVITIDKATG
210 220 230 240 250
KISKLGRSFT RARDYDAMGA QTQFVQCPEG ELQKRKEVVH TVSLHEIDVI
260 270 280 290 300
NSRTQGFLAL FSGDTGEIKS EVREQINAKV SEWREEGKAE IIPGVLFIDE
310 320 330 340 350
VHMLDIECFS FLNRALESDL SPVLIMATNR GITRIRGTNY QSPHGIPIDM
360 370 380 390 400
LDRLLIIATT PYTEKETRQI LKIRCEEEDV ELSEEAHTVL TRIGQETSLR
410 420 430 440 450
YAIQLISTAG LVCRKRRGTE VQVEDIKRVY SLFLDEARSS QYMKEYQDSF
460
LFNETQTSQM DTS
Length:463
Mass (Da):51,251
Last modified:June 1, 2003 - v1
Checksum:i517DE09F228AB61D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057075 mRNA. Translation: AAL18005.1.
BC058871 mRNA. Translation: AAH58871.1.
RefSeqiNP_777285.1. NM_174860.2.
UniGeneiDr.35479.

Genome annotation databases

EnsembliENSDART00000078018; ENSDARP00000072482; ENSDARG00000055639.
GeneIDi317678.
KEGGidre:317678.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057075 mRNA. Translation: AAL18005.1.
BC058871 mRNA. Translation: AAH58871.1.
RefSeqiNP_777285.1. NM_174860.2.
UniGeneiDr.35479.

3D structure databases

ProteinModelPortaliP83571.
SMRiP83571. Positions 132-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000072482.

Proteomic databases

PaxDbiP83571.
PRIDEiP83571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000078018; ENSDARP00000072482; ENSDARG00000055639.
GeneIDi317678.
KEGGidre:317678.

Organism-specific databases

CTDi10856.
ZFINiZDB-GENE-030109-1. ruvbl2.

Phylogenomic databases

eggNOGiKOG2680. Eukaryota.
COG1224. LUCA.
GeneTreeiENSGT00550000075034.
HOGENOMiHOG000190885.
HOVERGENiHBG054186.
InParanoidiP83571.
KOiK11338.
OMAiYDAMGAQ.
OrthoDBiEOG75TMBR.
PhylomeDBiP83571.
TreeFamiTF300469.

Miscellaneous databases

PROiP83571.

Gene expression databases

BgeeiP83571.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR027238. RuvB-like.
IPR010339. TIP49_C.
[Graphical view]
PANTHERiPTHR11093. PTHR11093. 1 hit.
PfamiPF06068. TIP49. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Reptin and pontin antagonistically regulate heart growth in zebrafish embryos."
    Rottbauer W., Saurin A.J., Lickert H., Shen X., Burns C.G., Wo Z.G., Kemler R., Kingston R., Wu C., Fishman M.
    Cell 111:661-672(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-190.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: AB.

Entry informationi

Entry nameiRUVB2_DANRE
AccessioniPrimary (citable) accession number: P83571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.