Reviewed, UniProtKB/Swiss-Prot P83564 (GPX1_CHLRE)

Names and origin

Protein names

Recommended name:
    Glutathione peroxidase, mitochondrial
    EC=1.11.1.9
Alternative name(s):
    CrGPx

Gene names

Name:

GPX

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O.
Subcellular location	MitochondrionProbable.
Sequence similarities	Belongs to the glutathione peroxidase family.
Mass spectrometry	Molecular weight is 18496 Da from positions 28 - 201. Determined by MALDI. Ref.1 Molecular weight is 18571 Da from positions 28 - 201. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Selenocysteine
Domain	Transit peptide
Ligand	Selenium
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	mitochondrion Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	glutathione peroxidase activity Ref.1 Inferred from direct assay. Source: UniProtKB selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion Potential
	Chain	28 – 201	174	Glutathione peroxidase, mitochondrial		PRO_0000013089
Amino acid modifications
	Non-standard residue	75	1	Selenocysteine

Sequences

	Sequence		Length	Mass (Da)
	P83564-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 1030F45EBA62C219	FASTA	201	21,499
	10 20 30 40 50 60 MLLTRKNVAV RPARAARRDV RAMSLLGNLF GGGSKPTSST SNFHQLSALD IDKKNVDFKS 70 80 90 100 110 120 LNNRVVLVVN VASKUGLTAA NYKEFATLLG KYPATDLTIV AFPCNQFGGQ EPGTNAEIKA 130 140 150 160 170 180 FASARGFSGA GALLMDKVDV NGANASPVYN FLKVAAGDTS DIGWNFGKFL VRPDGTVFGR 190 200 YAPTTGPLSL EKYIVELINS R

References

[1]

"A selenoprotein in the plant kingdom. Mass spectrometry confirms that an opal codon (UGA) encodes selenocysteine in Chlamydomonas reinhardtii glutathione peroxidase." Fu L.-H., Wang X.-F., Eyal Y., She Y.-M., Donald L.J., Standing K.G., Ben-Hayyim G. J. Biol. Chem. 277:25983-25991(2002) [PubMed: 11973339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SELENOCYSTEINE AT SEC-75, MASS SPECTROMETRY. Strain: 2137.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY051144 mRNA. Translation: AAL14348.1.
RefSeq	XP_001701658.1.
UniGene	Cre.4579
3D structure databases
HSSP	HSSP built from PDB template 1GP1 based on UniProtKB P00435.
ModBase	Search...
Protein family/group databases
PeroxiBase	2590. CreGPx02.
Genome annotation databases
GeneID	5727250.
KEGG	cre:CHLREDRAFT_206090.
Family and domain databases
InterPro	IPR000889. Glut_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX1_CHLRE

Accession

Primary (citable) accession number: P83564

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	February 26, 2008
Last modified:	November 25, 2008
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)