Reviewed, UniProtKB/Swiss-Prot P83563 (ALL6_APIME)

Names and origin

Protein names	Recommended name:     Allergen Api m 6 Alternative name(s):     Allergen=Api m 6
Organism	Apis mellifera (Honeybee)
Taxonomic identifier	7460 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Apoidea › Apidae › Apis

Protein attributes

Sequence length	71 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Secreted › extracellular space.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. This is a venom allergen. Ref.1
Miscellaneous	Four isoforms exist; 6.01, 6.02, 6.03 and 6.04. This is isoform 6.03. Ref.1
Sequence similarities	Contains 1 TIL (trypsin inhibitory-like) domain.
Mass spectrometry	Molecular mass is 7190 Da from positions 1 - 67. Determined by MALDI. Variant 6.01. Ref.1 Molecular mass is 7400 Da from positions 1 - 69. Determined by MALDI. Variant 6.02. Ref.1 Molecular mass is 7598 Da from positions 1 - 71. Determined by MALDI. Variant 6.03. Ref.1 Molecular mass is 7808 Da from positions 1 - 73. Determined by MALDI. Variant 6.04. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Disease	Allergen
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 71	71	Allergen Api m 6		PRO_0000174335
Regions
	Domain	16 – 70	55	TIL
Amino acid modifications
	Disulfide bond	16 ↔ 50		By similarity
	Disulfide bond	25 ↔ 46		By similarity
	Disulfide bond	29 ↔ 42		By similarity
	Disulfide bond	33 ↔ 70		By similarity
	Disulfide bond	52 ↔ 64		By similarity
Natural variations
	Natural variant	1 – 4	4	Missing in Api m 6.01 and Api m 6.02. Ref.1
	Natural variant	71	1	G → GLP in Api m 6.02 and Api m 6.04. Ref.1
	Natural variant	71	1	G → GPL in Api m 6.02 and Api m 6.04.

Sequences

	Sequence		Length	Mass (Da)
	P83563-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D619B9C3EE5886B7	FASTA	71	7,598
	10 20 30 40 50 60 FGGFGGFGGL GGRGKCPSNE IFSRCDGRCQ RFCPNVVPKP LCIKICAPGC VCRLGYLRNK 70 KKVCVPRSKC G

References

[1]	"Api m 6: a new bee venom allergen." Kettner A., Hughes G.J., Frutiger S., Astori M., Roggero M., Spertini F., Corradin G. J. Allergy Clin. Immunol. 107:914-920(2001) [PubMed: 11344362] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY, ALLERGENICITY. Tissue: Venom.

Cross-references

Sequence databases
UniGene	Ame.8030
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002919. Prot_Inh_CR_TIL. IPR018453. Prot_Inh_CR_TIL_sg. [Graphical view]
Pfam	PF01826. TIL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALL6_APIME

Accession

Primary (citable) accession number: P83563

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	June 1, 2003
Last modified:	November 24, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)