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Protein

Beta-hexatoxin-Mg1a

Gene
N/A
Organism
Macrothele gigas (Spider)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Insect and vertebrate active toxin. Binds to site 4 of mammalian voltage-gated sodium channels and shifts the activation voltage of the mammalian Nav1.2a/SCN2A channel to more hyperpolarized voltages, whereas the insect channel, DmNav1 (para), is not affected. Competes for binding at site 3 of the insect sodium channel. Causes temporary paralysis when injected into lepidopteran larvae at 8.6 nmol/g. A low intracranial injection dose into mice causes lacrimation, closure of the eyes and sweating. A high injection dose causes extensive lacrimation and death.1 Publication

Miscellaneous

The primary structure of the mature toxin is identical to that of beta-hexatoxin-Mr1a (Raventoxin-III) from Macrothele raveni (AC P61232).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexatoxin-Mg1a
Short name:
Beta-HXTX-Mg1a
Alternative name(s):
Neurotoxin magi-5
OrganismiMacrothele gigas (Spider)
Taxonomic identifieri223896 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeHexathelidaeMacrothele

Organism-specific databases

ArachnoServeriAS000384. beta-hexatoxin-Mg1a.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.73 pmol/g to mice by intracranial injection.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55T → A: Decrease in activity. 1 Publication1
Mutagenesisi56F → A: Decrease in activity. 1 Publication1
Mutagenesisi56F → Y: Loss of activity on Nav1.2. 1 Publication1
Mutagenesisi57W → A or Y: Decrease in activity. 1 Publication1
Mutagenesisi58K → A: No change in activity. 1 Publication1
Mutagenesisi60K → A: Loss of activity on Nav1.2. 1 Publication1
Mutagenesisi61N → A: No change in activity. 1 Publication1
Mutagenesisi62K → A: Decrease in activity. 1 Publication1
Mutagenesisi63K → A: No change in activity. 1 Publication1
Mutagenesisi64E → A: Loss of activity on Nav1.2. 1 Publication1
Mutagenesisi68W → A or F: Loss of activity on Nav1.2. 1 Publication1
Mutagenesisi68W → Y: Decrease in activity. 1 Publication1
Mutagenesisi69N → A: Loss of activity on Nav1.2. 1 Publication1
Mutagenesisi73L → A: Decrease in activity. 1 Publication1
Mutagenesisi75I → A: Loss of activity on Nav1.2. 1 Publication1
Mutagenesisi77M → A: Loss of activity on Nav1.2. 1 Publication1
Mutagenesisi78P → A: Loss of activity on Nav1.2. 1 Publication1
Mutagenesisi79R → A: Important increase in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000003558821 – 502 PublicationsAdd BLAST30
PeptideiPRO_000003558951 – 79Beta-hexatoxin-Mg1a1 PublicationAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 661 Publication
Disulfide bondi59 ↔ 711 Publication
Disulfide bondi65 ↔ 761 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

Secondary structure

179
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi55 – 57Combined sources3
Beta strandi64 – 67Combined sources4
Beta strandi73 – 76Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GX1NMR-A51-79[»]
ProteinModelPortaliP83561.
SMRiP83561.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83561.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.Sequence analysis

Sequence similaritiesi

Belongs to the magi-5 family.Sequence analysis

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR012628. Toxin_23.
PfamiView protein in Pfam
PF08093. Toxin_23. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAPATTLIL VMSLISVLWA TPDLEEGDLL AELGDLIATD DEYPMKPEER
60 70
GCKLTFWKCK NKKECCGWNA CALGICMPR
Length:79
Mass (Da):8,791
Last modified:February 1, 2005 - v2
Checksum:i1C07F3B96D5AA901
GO

Mass spectrometryi

Molecular mass is 3286.8 Da from positions 51 - 79. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB121201 mRNA. Translation: BAD13408.1.

Similar proteinsi

Entry informationi

Entry nameiTXMG5_MACGS
AccessioniPrimary (citable) accession number: P83561
Secondary accession number(s): Q75WH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: February 1, 2005
Last modified: November 2, 2016
This is version 67 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families