Reviewed, UniProtKB/Swiss-Prot P83542 (PA1_POLGA)

Names and origin

Protein names	Recommended name:     Phospholipase A1     EC=3.1.1.32 Alternative name(s):     Allergen=Pol g 1
Organism	Polistes gallicus (Paper wasp)
Taxonomic identifier	34730 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Polistinae › Polistini › Polistes

Protein attributes

Sequence length	42 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolysis of phosphatidylcholine with phospholipase A1 (EC 3.1.1.32) activity. Has weak hemolytic activity By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Contains six disulfide bonds By similarity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.
Mass spectrometry	Molecular mass is 33475 Da from positions 1 - ?. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Disease	Allergen
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Non-traceable author statement. Source: UniProtKB type I hypersensitivity Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular region Non-traceable author statement. Source: UniProtKB
Molecular function	phosphatidylserine-specific phospholipase A1 activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›42	›42	Phospholipase A1		PRO_0000090377
Experimental info
	Non-terminal residue	42	1

Sequences

	Sequence		Length	Mass (Da)
	P83542-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 524589208F2113C3	FASTA	42	4,985
	10 20 30 40 GITPDCTFNE KDIELHVYSR DKRNGIILKK EILKNYDLFK ES

References

[1]

"Characterization of the major allergens purified from the venom of the paper wasp Polistes gallicus." Pantera B., Hoffman D.R., Carresi L., Cappugi G., Turillazzi S., Manao G., Severino M., Spadolini I., Orsomando G., Moneti G., Pazzagli L. Biochim. Biophys. Acta 1623:72-81(2003) [PubMed: 14572904] [Abstract] Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBUNIT, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.32. 289561.
Family and domain databases
InterPro	IPR008262. Lipase_Ser_AS. [Graphical view]
PROSITE	PS00120. LIPASE_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA1_POLGA

Accession

Primary (citable) accession number: P83542

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	September 22, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)